scholarly journals Propagación in vitro de especies de Echinocereus, Escontria, Mammillaria, Melocactus y Polaskia (Cactaceae)

2017 ◽  
pp. 9
Author(s):  
José Luis Retes-Pruneda ◽  
María de Lourdes Valadez-Aguilar ◽  
Martha Evelia Pérez-Reyes ◽  
Eugenio Pérez-Molphe-Balch
Keyword(s):  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Basal Medium ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Indolebutyric Acid ◽  
Multiple Shoot Formation ◽  
Escontria Chiotilla

In vitro propagation systems by means of areole activation were developed for Echinocereus knippelianus, Echinocereus schmollii, Mammillaria carmenae, M. carmenae fo. rubrisprina, M. herrerae, M. theresae, Melocactus curvispinus, Escontria chiotilla and Polaskia chichipe. In vitro germinated seedlings were used as source of explants. Multiple shoot formation from areoles was achieved on MS basal medium supplemented with 3% sucrose, 10 g L-1 agar and 6-benzylaminopurine (BA) or 6-(, -dimethylallylamino)purine (2iP). Efficiencies ranged from 6.0 shoots per explant in M. carmenae fo. rubrisprina to 13.5 shoots per explant in Echinocereus schmollii. Rooting of the in vitro generated shoots was achieved in MS basal medium, or MS basal medium supplemented with indoleacetic acid, indolebutyric acid or activated charcoal. Finally, 49-98% of these plants survived.

scholarly journals Micropropagation of Yellow Passionfruit by Axillary Bud Proliferation

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1276-1277 ◽  
Author(s):  
Joao L.C. Faria ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Apices ◽  
Passiflora Edulis ◽  
Multiple Shoot Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

A protocol for in vitro propagation in yellow passionfruit (Passiflora edulis F. flavicarpa Deg) has been developed. Shoot apices from aseptically grown seedlings were used as initial explants. Multiple shoot formation was obtained by placing the explants on solidified Murashige and Skoog medium containing BA. Regenerated shoots were rooted on media without growth regulators. Following conventional procedures, plantlets were transferred to soil with more than 90% success. Chemical name used: N-(phenylmethyl)-lH-purin-6-amine (BA).

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

scholarly journals Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

2013 ◽  
Vol 1 (1-2) ◽  
pp. 69-72 ◽  
Author(s):  
Nayem Zobayer ◽  
Shamsul H Prodhan ◽  
Saif U Sikdar ◽  
Fazle Azim ◽  
M Ashrafuzzaman
Keyword(s):  
Promising Result ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Fragaria Ananassa ◽  
Benzyl Adenine ◽  
Multiple Shoot Formation ◽  
Basal Media ◽  
Hormonal Concentration

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13936 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 69-72, December, 2011

scholarly journals In Vitro Propagation of Viburnum opulus ‘Nanum’

1985 ◽  
Vol 3 (2) ◽  
pp. 41-45
Author(s):  
Virginia Hildebrandt ◽  
Patricia M. Harney
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Viburnum Opulus ◽  
Adenine Sulfate ◽  
Growing Shoot

Explants of actively growing shoot tips from greenhouse-grown plants of Viburnum opulus ‘Nanum’ initiated new shoots on a modified Murashige and Skoog (MS) revised medium plus 0.1 mg/L indoleacetic acid (IAA). These shoots were transferred for proliferation to the same medium, but with 1 mg/L 6-benzylamino purine (BA) replacing IAA and the addition of 2.5 mg/L 2-iso-pentenyladenine (2iP). Both adenine sulfate AdS) and NaH2PO4.H2O inhibited shoot proliferation, while gibberellic acid (GA3) and glycine had no effect. The shoots could be rooted either in the basal medium without cytokinin or in vermiculite under mist.

Electron Microscope studies (TEM and SEM) of in vitro multiplication of Lavandula stoechas L.: I. regeneration from seedling shoot apex explants

1990 ◽  
Vol 48 (3) ◽  
pp. 684-685
Author(s):  
J. F. Mesquita ◽  
M. L. Guimarães ◽  
J.D. Santos Dias
Keyword(s):  
Shoot Apex ◽  
Basal Medium ◽  
Shoot Formation ◽  
Distal Segment ◽  
Multiple Shoot ◽  
Fluorescent Light ◽  
Lavandula Stoechas ◽  
Shoot Apex Explants ◽  
Electron Microscopes

Available data concerning “in vitro” multiplication of Lavandula sps. are scarce and do not involve cytological studies. In this work, shoot initiation from shoot apex explants of Lavandula stoechas is analysed, for the first time, in light and electron microscopes (TEM, SEM). Following habitual sterilization, seeds of Lavandula stoechas were germinated on MS basal medium with 2% sucrose (W/v). From 9-days old seedlings, explants (shoot tips including a small distal segment of hypocotyl - fig. 1) were aseptically excised and cultivated in that basal medium added with organic components as in B5 medium of Gamborg et al., 10% (V/v) of coconut milk, 0.8% of agar and benzyl-amino-purine (BAP) at different concentrations (0-4 mg/1). The pH was adjusted to 5.6 and cultures were maintained at 25±1°C with fluorescent light (2000 lux) for 16 h/day.High frequency of multiple shoot formation was achieved on media supplemented with BAP, the concentration of 1mg/l showing to be the optimal one (Table 1).

scholarly journals In vitro Regeneration and Flower Induction on Solanum nigrum L. from Pachamalai hills of Eastern Ghats

1970 ◽  
Vol 18 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Amzad Basha Kolar ◽  
L . Vivekanandan ◽  
Ghouse Basha M
Keyword(s):  
In Vitro Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Solanum Nigrum ◽  
In Vitro Flowering ◽  
Eastern Ghats ◽  
Solanum Nigrum L

 Explants of Solanum nigrum L., collected from Pachamalai hills callused successfully on MS basal medium supplemented with IAA and BAP. The highest frequency of green compact callus and multiple shoots were obtained on MS containing 2.0 mg/l IAA and 0.5 mg/l BAP. The callus when cultured on MS basal medium fortified with different concentrations of BAP (3.0 - 8.0 mg/l) and IAA (0.5 mg/l) showed multiple shoot formation. The highest frequency of multiple shoots was obtained on MS containing 6.0 mg/l BAP and 0.5 mg/l IAA. For in vitro flowering, the node explants were cultured on MS fortified with different concentrations of BAP (2.0 - 7.0 mg/l) and NAA (0.5 mg/l). The highest number of multiple shoots were obtained in MS supplemented with 6.0 mg/l BAP and 0.5 mg/l NAA. The in vitro flowering was observed on MS containing 2,4-D and BAP 1.5 mg/l, respectively. The best rooting was obtained on MS containing 0.5 mg/l IBA. The well-rooted plants were hardened and finally planted in the garden.  Key words: In vitro studies, Medicinal plant, Solanum nigrum, Node, Callus D.O.I. 10.3329/ptcb.v18i1.3264 Plant Tissue Cult. & Biotech. 18(1): 43-48, 2008 (June)

scholarly journals Micropropagation of Ilex glabra (L.) A. Gray

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 805-808 ◽  
Author(s):  
Youping Sun ◽  
Donglin Zhang ◽  
John Smagula
Keyword(s):  
Mass Production ◽  
Adventitious Roots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Bud ◽  
Nodal Segments ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Indolebutyric Acid

Nodal segments containing one axillary bud (1 to 1.5 cm) were disinfected using 10% bleach and were established on a Murashige and Skoog (MS) medium without hormones at 27 °C and with a 16-h photoperiod. The sprouted shoots (≈1.0 cm) were cultured on a MS medium supplemented with 6-benzylaminopurine (BAP), kinetin (KIN), or zeatin (ZT) at 2.3, 4.5, 9.1, or 18.2 μM. After 38 d, ZT and BAP significantly induced multiple shoot formation with multiplication rates of 4 to 6, whereas the multiplication rate of KIN was less than 2. Shoots cultured on ZT grew significantly taller than those on BAP and KIN. The height of the longest shoots treated with ZT was 4.6 cm, which was 1.6 to 2.2 times greater than those treated with BAP or KIN. To induce rooting, shoots (≈2 cm) were subcultured on one-fourth strength MS (1/4 MS) medium containing either 3-indolebutyric acid (IBA) or 1-naphthylacetic acid (NAA) at 2.6, 5.1, or 10.3 μM. Adventitious roots formed in vitro after 2 to 4 weeks. IBA at 10.3 μM produced the best rooting (100%) compared with other treatments after 38 d of culture. The average number of roots per shoot for IBA was ≈15, which was 1.6 to 3.1 times as many as that of other treatments. All rooted plantlets were then transplanted into a mix of peatmoss and perlite (1:1 v/v) and acclimatized in a mist system. Average plantlet survival was 73.6% after 35 d. After acclimatization, they were grown in a pot with Metro-mix under greenhouse conditions for 10 weeks where 95% of plants survived and grew up to 6.8 cm high. The micropropagation procedure, i.e., nodal segments containing one axillary bud proliferated on MS with 4.5 μM ZT followed by in vitro rooting on 1/4 MS plus 10.3 μM IBA, could be used for commercial mass production of new inkberry cultivars.

scholarly journals In Vitro Propagation of Three Species of Columnar Cacti from the Sonoran Desert

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 693-696 ◽  
Author(s):  
Eugenio Pérez-Molphe-Balch ◽  
Martha Evelia Pérez-Reyes ◽  
Carlos Antonio Dávila-Figueroa ◽  
Enrique Villalobos-Amador
Keyword(s):  
Sonoran Desert ◽  
In Vitro Propagation ◽  
Production Efficiency ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Columnar Cacti ◽  
Best Response ◽  
Stenocereus Thurberi ◽  
Basal Media

In vitro propagation systems were developed for Carnegiea gigantea (Engelm.) Britt & Rose, Pachycereus pringlei (Berger) Britt & Rose and Stenocereus thurberi (Engelm.) Buxb, three North American species of columnar cacti. In vitro germinated seedlings were used as a source of explants. Multiple shoot formation from areoles was achieved for three types of explants (apical, lateral, and transverse) cultured on Murashige and Skoog (MS) basal media supplemented with 3% sucrose, 10 g·L-1 agar and various treatments with growth regulators. The highest shoot production efficiency for C. gigantea was obtained on transverse explants cultured on a medium with 2 mg·μmL-1 (8.87 μm) BA, where 5.3 shoots per explant were obtained. In P. pringlei and S. thurberi the best response was obtained using transverse explants on medium with 1 mg·L-1 (4.44 μm) BA (3.8 and 4.3 shoots per explant, respectively). Rooting of the in vitro generated shoots was achieved most efficiently on MS basal media with 3% sucrose, 10 g·L-1 agar and 1 mg·L-1 (4.9 μm) indole-3-butyric acid. Rooting frequencies were 92%, 88%, and 96% for C. gigantea, P. pringlei and S. thurberi, respectively, and the frequency of survival of the plants once transferred to soil was 86% on average. Chemical name used: benzyladenine (BA).

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