Interactions of growth regulators, explant age, and culture environment controlling organogenesis from lettuce cotyledons in vitro

1984 ◽  
Vol 62 (3) ◽  
pp. 586-590 ◽  
Author(s):  
David T. Webb ◽  
Luis D. Torres ◽  
Pierre Fobert
Keyword(s):  
Growth Regulators ◽  
Indoleacetic Acid ◽  
Low Frequency ◽  
Shoot Initiation ◽  
Lactuca Sativa L ◽  
Grand Rapids ◽  
Environment Control ◽  
Culture Environment ◽  
Bud Initiation

Organogenesis in cultured Lactuca sativa L. cv. Grand Rapids cotyledons was controlled by growth regulators and influenced by explant age as well as culture environment. Control explants formed roots with a low frequency. Exposure to indoleacetic acid (IAA) greatly stimulated root initiation. Kinetin (K) applied singly or in combination with IAA induced bud formation. Benzylaminopurine (BA) also caused bud initiation. Root and shoot initiation was optimal with 4-day-old explants. Organogenesis declined with older explants. More buds formed in light than in darkness. Other environmental variables tested had no marked effect on K-induced bud initiation. Control explants on medium containing no growth regulator never formed buds.

scholarly journals In vitro conservation of diploid banana accessions

2000 ◽  
Vol 57 (2) ◽  
pp. 245-249 ◽  
Author(s):  
Roberto Pedroso de Oliveira ◽  
Sebastião de Oliveira e Silva ◽  
Keuder Magalhães da Silva ◽  
Daniela Garcia Silveira
Keyword(s):  
Light Intensity ◽  
Growth Regulators ◽  
In Vitro Conservation ◽  
Ms Medium ◽  
Long Time ◽  
The Mean ◽  
Culture Environment ◽  
Lower Temperature ◽  
Diploid Banana

A protocol for the in vitro conservation of diploid banana accessions based on lower temperature for culture environment was developed. Twenty four genotypes, four clones per genotype were studied. After disinfection, shoot tips (0.5 cm diameter x 0.5 cm height) were established in vitro and submitted to multiplication induced by benzylaminopurine. Twelve explants (0.6 cm diameter x 1.2 cm height) from each accession were transferred to the MS medium supplemented with 20 g L-1 sucrose and 2 g L-1 Phytagel, without growth regulators, under light intensity of 2000 lux, 16-hour photoperiod, at temperatures of: 17 ± 2ºC, 22 ± 2ºC and 26 ± 2ºC. The development of plantlets was evaluated during 450 days and the survived plantlets were acclimated under greenhouse conditions. There were no differences among genotypes concerning the behavior during in vitro conservation; all genotypes showed potential to be conserved for a long time. The interval for transference should be 180, 360 and 450 days under the mean temperatures of 26, 22 and 17ºC, respectively.

EFFECT OF PLANT GROWTH REGULATORS ON THE INFECTIVITY OF TOBACCO MOSAIC VIRUS

1964 ◽  
Vol 42 (7) ◽  
pp. 885-889 ◽  
Author(s):  
Victor Weeraratne ◽  
Avery E. Rich
Keyword(s):  
Growth Regulators ◽  
Room Temperature ◽  
Indoleacetic Acid ◽  
Inhibitory Effect ◽  
Indolebutyric Acid ◽  
Meat Extract ◽  
Naturally Occurring ◽  
Chlorophenoxyacetic Acid ◽  
Coconut Meat

A naturally occurring growth regulator, coconut meat extract, was tested for the inhibition of tobacco mosaic (TMV) virus infection. Coconut meat extract inhibited infection by 97%. The inhibitor in coconut meat extract was effective when the virus inoculation took place 8 days after the application of the inhibitor, but the inhibitor was ineffective when applied after inoculation with the virus. Aging in vitro for 64 days at 4 °C and at room temperature did not reduce the inhibitory effect of coconut meat extract, but dilution markedly reduced its inhibitory effect. Three commercially available growth regulators, indoleacetic acid, indolebutyric acid, and p-chlorophenoxyacetic acid, were tested at a concentration of 100 p.p.m., but their inhibiting activity was very low.

scholarly journals Standardization of plant growth regulators for successful tissue culture of sweet potato

2018 ◽  
Vol 16 (2) ◽  
pp. 178-181
Author(s):  
Jahanara Parvin ◽  
Mahbub Robbani ◽  
Md Fakhrul Hasan ◽  
Farhana Hoque
Keyword(s):  
Sweet Potato ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Naphthalene Acetic Acid ◽  
Nodal Segment ◽  
Ms Medium ◽  
Root Initiation ◽  
Shoot Initiation ◽  
Minimum Number

The experiment was carried out during the period from June 2016 to January, 2017 at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University (PSTU) for in vitro regeneration of sweet potato. In this study, the nodal segment explants and different combinations of growth regulators were used for in vitro regeneration of sweet potato. BAP (6-benzylamino purine) and KIN (Kinetin) were used for in vitro shoot regeneration and IBA (Indole-6- Butyric Acid) with NAA (Naphthalene Acetic Acid) were used for in vitro root regeneration. The highest percentage of shoot initiation (91.30), the minimum number of days (9.00) for shoot initiation, the highest number of shoot/plantlet (11.00) and the highest shoot length (4.38 cm) was observed when nodal segment explants cultured on MS medium supplemented with BAP 1.5 mg/L + KIN 0.1 mg/L. On the other hand, the highest root initiation percentage (94.12), the minimum number of days (6.00) for root initiation, the highest number of root/plantlet (9.33) and the highest root length (11.13 cm) was observed in the MS medium supplemented with IBA 0.5 mg/L + NAA 0.1 mg/L. Regenerated plantlets were acclimatized for 5 days and adopted in soil by 6-7 days of transplantation.J. Bangladesh Agril. Univ. 16(2): 178-181, August 2018

scholarly journals Pertumbuhan Tanaman Selada (Lactuca sativa L. var. New Grand Rapids) menggunakan Teknologi Hidroponik Sistem Terapung (THST) Tanpa Sirkulasi dengan Penambahan Giberelin (GA3)

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Widya Sri Rahayu Mukarlina Riza Linda
Keyword(s):  
Growth Regulators ◽  
Lactuca Sativa ◽  
Natural Sciences ◽  
Fresh Weight ◽  
Technology System ◽  
Lactuca Sativa L ◽  
Randomized Design ◽  
Grand Rapids ◽  
Number Of Leaves ◽  
Completely Randomized Design

Lettuce (Lactuca sativa L. var. New Grand Rapids) is a vegetable plant that has a contains high nutrients. Efforts that can increase the production of lettuce is using hydroponics technology and the addition of growth regulators is gibberellin (GA3). This research aims to determine the effect of addition of GA3 to gowth of lettuce using floating technology system of hydroponics without circulation. The research was conducted for four months from May to August 2017 in the Green House and Laboratory of Biology of the Faculty of Mathematics and Natural Sciences, Tanjungpura University Pontianak. This research used a completely randomized design with five treatments (K) and three repetitions. The treatments given is the addition of GA3 with different consentrations: K0 (Control), K1 (5 ppm), K2 (10 ppm), K3 (15 ppm), and K4 (20 ppm). The result showed that the addition of GA3 didn’t significant affect on the number of leaves, but showed a significantly affected the height, root lenght, leaf areas, and fresh weight.

scholarly journals Growth regulator effect on in vitro regeneration of rhododendron cultivars

2008 ◽  
Vol 35 (No. 2) ◽  
pp. 90-94 ◽  
Author(s):  
H. Vejsadová
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Indoleacetic Acid ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Commercial Preparation ◽  
High Level

In Rhododendron L. cv. Azuro, Bohumil Kavka, Catharine van Toll, Grandiflorum, Mars, Nova Zembla, Ortrud, Ovation, Prof. Scholz, Purple Splendour, Rebe and Van Werden Poelman, the effect of growth regulators on organogenesis induction of shoot-tip meristems was tested. All cultivars significantly showed the highest shoot regeneration on MS medium containing 6 mg/dm<sup>3</sup> isopentenyladenine (2iP). For most rhododendrons, the highest shoot multiplication was found on a medium with 8&ndash;10 mg/dm<sup>3</sup> 2iP in combination with 1 mg/dm<sup>3</sup> indoleacetic acid (IAA). Shoots rooted successfully in the substrate with high level of peat without growth regulators. However, the commercial preparation Racine significantly increased rooting in cv. Grandiflorum, Nova Zembla and Rebe compared with 0.03% indolebutyric acid (IBA).

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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