Warsaw students visit Bristol for exchange event

2018 ◽  
Vol 182 (21) ◽  
pp. 589.2-589
Keyword(s):  
Exchange Event

Epinephrine syringe exchange events in a paediatric cardiovascular ICU: analysing the storm

2017 ◽  
Vol 28 (3) ◽  
pp. 409-415 ◽  
Author(s):  
Barbara-Jo Achuff ◽  
Jameson C. Achuff ◽  
Hwan H. Park ◽  
Brady Moffett ◽  
Sebastian Acosta ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Systolic Blood Pressure ◽  
Vital Signs ◽  
Syringe Exchange ◽  
Control Data ◽  
Flow Change ◽  
Exchange Event ◽  
The Hours ◽  
Pressure Perturbations

AbstractIntroductionHaemodynamically unstable patients can experience potentially hazardous changes in vital signs related to the exchange of depleted syringes of epinephrine to full syringes. The purpose was to determine the measured effects of epinephrine syringe exchanges on the magnitude, duration, and frequency of haemodynamic disturbances in the hour after an exchange event (study) relative to the hours before (control).Materials and methodsBeat-to-beat vital signs recorded every 2 seconds from bedside monitors for patients admitted to the paediatric cardiovascular ICU of Texas Children’s Hospital were collected between 1 January, 2013 and 30 June, 2015. Epinephrine syringe exchanges without dose/flow change were obtained from electronic records. Time, magnitude, and duration of changes in systolic blood pressure and heart rate were characterised using Matlab. Significant haemodynamic events were identified and compared with control data.ResultsIn all, 1042 syringe exchange events were found and 850 (81.6%) had uncorrupted data for analysis. A total of 744 (87.5%) exchanges had at least 1 associated haemodynamic perturbation including 2958 systolic blood pressure and 1747 heart-rate changes. Heart-rate perturbations occurred 37% before exchange and 63% after exchange, and 37% of systolic blood pressure perturbations happened before syringe exchange, whereas 63% occurred after syringe exchange with significant differences found in systolic blood pressure frequency (p<0.001), duration (p<0.001), and amplitude (p<0.001) compared with control data.ConclusionsThis novel data collection and signal processing analysis showed a significant increase in frequency, duration, and magnitude of systolic blood pressure perturbations surrounding epinephrine syringe exchange events.

scholarly journals Suzaku detection of enigmatic geocoronal solar wind charge exchange event associated with coronal mass ejection

2019 ◽  
Vol 71 (1) ◽  
Author(s):  
Daiki Ishi ◽  
Kumi Ishikawa ◽  
Masaki Numazawa ◽  
Yoshizumi Miyoshi ◽  
Naoki Terada ◽  
...  
Keyword(s):  
Solar Wind ◽  
Coronal Mass Ejection ◽  
Charge Exchange ◽  
Exchange Event ◽  
Solar Wind Charge Exchange ◽  
Mass Ejection

scholarly journals Cloning and expression of maltooligosyltrehalose trehalohydrolase from Sulfolobus solfataricus DSM 1616 in Bacillus subtilis WB800

2020 ◽  
Vol 18 (2) ◽  
pp. 363-372
Author(s):  
Nguyen Tien Cuong ◽  
Nguyen Thi Hien Trang ◽  
Nguyen Thi Thao ◽  
Le Thanh Hoang ◽  
Nguyen Sy Le Thanh ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Protein ◽  
Amylase Activity ◽  
Expression System ◽  
Sulfolobus Solfataricus ◽  
Cloning And Expression ◽  
Exchange Event ◽  
Sds Page ◽  
Dna Fragment ◽  
Conventional Expression

Maltooligosyltrehalose trehalohydrolase (MTHase) is an industrial enzyme for the production of trehalose. A DNA fragment of 1680 bp encoding for MTHase was cloned from Sulfobolus solfataricus DSM 1616 then fused with promoter acoA-amyE already amplified from pMSE3 vector by PCR to generate an expression cassette acoMTH. Afterward the cassette was inserted into pAC7 vector for expression of the gene in Bacillus subtilis WB800 – a conventional expression system. Gene MTH was inserted into the genome of B. subtilis WB800 by cross-exchange event of pAC7 vector with the host genome for expression of high quality and high quantity of extracellular recombinant protein. By crossing-exchange event at 3’amyE-5’amyE, the expressional cassette was integrated into B. subtilis WB800 genome. The expressional cassette was integrated into B. subtilis WB800 genome replacing 3’amyE-5’amyE, hindering the native amylase activity of the host. Expression of expected protein was confirmed by electrophoresis SDS-PAGE. From our results, it indicates that gene MTH was expressed successfully in B. subtilis WB800. After 0.5% acetoin induction for 48 h, the data showed that the protein with a molecular mass of ~64 kDa on SDS-PAGE was expressed. The level of recombinant protein in WBpAacoMTH was increased and reached 2.5%, 15.2% and 21.95%, respectively comparing with native B. subtilis WB800.

Meiotic exchange event within the stalk region of an inverted chromosome 22 results in a recombinant chromosome with duplication of the distal long arm

2005 ◽  
Vol 138A (4) ◽  
pp. 355-360 ◽  
Author(s):  
Luke J. Boyd ◽  
Joseph S. Livingston ◽  
Michael G. Brown ◽  
Helen J. Lawce ◽  
Joseph T. Gilhooly ◽  
...  
Keyword(s):  
Chromosome 22 ◽  
Recombinant Chromosome ◽  
Exchange Event

Two kinds of "recombination nodules" in Neurospora crassa

Genome ◽  
1989 ◽  
Vol 32 (2) ◽  
pp. 309-317 ◽  
Author(s):  
Maja Bojko
Keyword(s):  
Neurospora Crassa ◽  
Synaptonemal Complex ◽  
Nucleolus Organizer ◽  
Crossing Over ◽  
Dramatic Reduction ◽  
Recombination Nodules ◽  
Exchange Event ◽  
The Mean ◽  
Different Origin ◽  
Positive Interference

Two morphological types of recombination nodules, termed early and late, are recognized in Neurospora crassa. Eighty nuclei at different substages were used to determine numbers of nodules per nucleus, distribution of nodules along the nucleolus-organizing chromosome, and distribution of nodules among the two largest chromosomes. Early nodules appear at the synaptonemal complex at early zygotene and increase in number during zygotene until a dramatic reduction occurs at zygotene – pachytene transition. Thereafter early nodules are steadily eliminated until they disappear by diplotene. Late nodules are also present during zygotene. Their number doubles at the zygotene – pachytene transition and stays at this level until diplotene. The total number of nodules is rather constant through zygotene and pachytene. Distribution of bivalents with 0, 1, 2, etc. nodules follows a Poisson distribution at zygotene, but not at pachytene, where variance is less than the mean, indicating positive interference. Nodules are distributed nonrandomly along the nucleolus-organizer bivalent. The pattern differs slightly in nuclei of different origin. Nuclei with unusual synaptonemal complexes sustain normal levels of recombination by having the same amount of nodules as normal nuclei. In abnormal nuclei nodules are preferentially associated with normal segments. It is proposed that early nodules do not participate in any form of recombination but have a role in finding an appropriate site for a crossing-over event. Morphological change to the late type indicates that the site has been reached and the exchange event can be mediated by the late nodule.Key words: recombination nodules, Neurospora crassa, synaptonemal complex.

scholarly journals Evidence for Acquisition of the Lipooligosaccharide Biosynthesis Locus in Campylobacter jejuni GB11, a Strain Isolated from a Patient with Guillain-Barré Syndrome, by Horizontal Exchange

2004 ◽  
Vol 72 (2) ◽  
pp. 1162-1165 ◽  
Author(s):  
Michel Gilbert ◽  
Peggy C. R. Godschalk ◽  
Marie-France Karwaski ◽  
C. Wim Ang ◽  
Alex van Belkum ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Dna Sequencing ◽  
Campylobacter Jejuni ◽  
Molecular Typing ◽  
Infected Patient ◽  
Outer Core ◽  
Strain Atcc ◽  
Sequencing Data ◽  
Class A ◽  
Exchange Event

ABSTRACT Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barré syndrome, has been shown to be genetically closely related to the completely sequenced strain C. jejuni NCTC 11168 by various molecular typing and serotyping methods. However, we observed that the lipooligosaccharide (LOS) biosynthesis genes strongly diverged between GB11 and NCTC 11168. We sequenced the LOS biosynthesis locus of GB11 and found that it was nearly identical to the class A LOS locus from the C. jejuni HS:19 Penner serotype strain (ATCC 43446). Analysis of the DNA sequencing data showed that a horizontal exchange event involving at least 14.26 kb had occurred in the LOS biosynthesis locus of GB11 between galE (Cj1131c in NCTC 11168) and gmhA (Cj1149 in NCTC 11168). Mass spectrometry of the GB11 LOS showed that GB11 expressed an LOS outer core that mimicked the carbohydrate portion of the gangliosides GM1a and GD1a, similar to C. jejuni ATCC 43446. The serum from the GB11-infected patient was shown to react with the LOS from both GB11 and ATCC 43446 but not with that from NCTC 11168. These data indicate that the antiganglioside response in the GB11-infected patient was raised against the structures synthesized by the acquired class A LOS locus.

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