Incidence of Mycobacterium avium subspecies paratuberculosis in bulk-tank milk samples from different regions in Switzerland

2002 ◽  
Vol 150 (7) ◽  
pp. 214-215 ◽  
Author(s):  
R. Stephan ◽  
K. Buhler ◽  
S. Corti
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

scholarly journals Mycobacterium avium subsp. paratuberculosis and M. a. avium detected by culture, IS900 and IS901 highly sensitive PCR in bulk tank milk from dairy herds in the Czech Republic between 2002 and 2004

2009 ◽  
Vol 27 (No. 5) ◽  
pp. 372-378 ◽  
Author(s):  
I. Slaná ◽  
M. Bartoš ◽  
P. Roubal ◽  
V. Babák ◽  
I. Pavlík
Keyword(s):  
Czech Republic ◽  
Mycobacterium Avium ◽  
Dairy Herds ◽  
Bulk Tank Milk ◽  
The Czech Republic ◽  
Milk Samples ◽  
Highly Sensitive ◽  
Bulk Tank ◽  
Cattle Farms

In this study, the possible presence was monitored of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (<i>MAP</i>) and <i>Mycobacterium avium</i> subspecies <i>avium</i> (<i>MAA</i>) by means of culture examination and PCR in 251 bulk tank milk samples from dairy herds in the Czech Republic between 2002 and 2004. The detection of <i>MAP</i> and <i>MAP</i> DNA in repeatedly collected bulk tank milk (BTM) samples from the selected cattle farms (seven farms) was the second purpose of the study. By culture, <i>MAP</i> was detected in 5 (2.0%) and <i>MAP</i> DNA in 85 (33.9%) of the total of 251 BTM samples. <i>MAA</i> was detected by culture and by PCR in 1 (0.4%) of the 251 BTM samples. This study demonstrates the presence of <i>MAP</i> and <i>MAA</i> in dairy herds in the Czech Republic.

Occurrence and characterization of ciprofloxacin‐resistant Escherichia coli from bovine and ovine bulk tank milk samples in Turkey

2021 ◽  
Author(s):  
Cemil Kürekci ◽  
Muhsin Aydın ◽  
İbrahim Ozan Tekeli ◽  
Pınar Ambarcıoğlu ◽  
Seydi Ahmet Şengül ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

scholarly journals Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 841
Author(s):  
Maria Liapi ◽  
George Botsaris ◽  
Costas Arsenoglou ◽  
Nikolas Markantonis ◽  
Christodoulos Michael ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cell ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

Characterization of the microbiota and resistome of bulk tank milk samples from Prince Edward Island dairy farms

2021 ◽  
Author(s):  
Landon M.C. Warder ◽  
Enrique Doster ◽  
Jennifer K. Parker ◽  
Paul S. Morley ◽  
J.T. McClure ◽  
...  
Keyword(s):  
Prince Edward Island ◽  
Dairy Farms ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank
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