scholarly journals Long-term detection of Mycobacterium avium subspecies paratuberculosis in individual and bulk tank milk from a dairy herd with a low prevalence of Johne’s disease

2013 ◽  
Vol 96 (6) ◽  
pp. 3517-3524 ◽  
Author(s):  
J.L. Khol ◽  
M. Wassertheurer ◽  
E. Sodoma ◽  
S. Revilla-Fernández ◽  
J. Damoser ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Dairy Herd ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Bulk Tank Milk ◽  
Bulk Tank ◽  
Low Prevalence

scholarly journals Low rate of detectable in utero transmission of Mycobacterium avium subspecies paratuberculosis in a dairy herd with a low prevalence of Johne’s disease

2011 ◽  
Vol 24 (1) ◽  
pp. 153-155 ◽  
Author(s):  
John M. Adaska ◽  
Robert H. Whitlock
Keyword(s):  
Mycobacterium Avium ◽  
Transmission Rate ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Dairy Herd ◽  
In Utero ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Causal Organism ◽  
Mycobacterium Avium Subsp Paratuberculosis

In an effort to correlate the likelihood of in utero transmission of Mycobacterium avium subsp. paratuberculosis (MAP), the causal organism of Johne’s disease, with the test status of the dam, tissues from neonatal calves borne to known test status cows were cultured for the presence of MAP. Tissues from a single calf borne to a test-positive cow shedding large numbers of organisms in the feces were positive for MAP. The detected overall transmission rate was approximately 2% (1/49), and the detected transmission rate in cows that were fecal culture positive and serum enzyme-linked immunosorbent assay suspect or positive was approximately 4.3% (1/23).

scholarly journals Membrane and Cytoplasmic Proteins of Mycobacterium avium subspecies paratuberculosis that Bind to Novel Monoclonal Antibodies

2018 ◽  
Vol 6 (4) ◽  
pp. 127 ◽  
Author(s):  
John Bannantine ◽  
Judith Stabel ◽  
John Lippolis ◽  
Timothy Reinhardt
Keyword(s):  
Monoclonal Antibodies ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Cell Extract ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mass Spectrometry Analysis ◽  
Lambda Phage ◽  
Expression Library ◽  
Spectrometry Analysis

Monoclonal antibodies against Mycobacterium avium subspecies paratuberculosis (Map) proteins are important tools in Johne’s disease research and diagnostics. Johne’s disease is a chronic inflammatory intestinal disease of cattle, sheep, and other ruminant animals. We have previously generated multiple sets of monoclonal antibodies (mAbs) in different studies; however, because many were generated and screened against a whole-cell extract of Map, the antigens that bind to these antibodies remained unknown. In this study, we used three different approaches to identify the corresponding Map antigens for 14 mAbs that could not be identified previously. In the first approach, a new Map-lambda phage expression library was screened to identify corresponding antigens for 11 mAbs. This approach revealed that mAbs 7C8, 9H3, 12E4, 3G5, and 11B8 all detect MAP_3404 encoding the biotin carboxylase subunit of acetyl-CoA carboxylase, while mAbs 7A6, 11F8, and 10C12 detect the GroEL2 chaperonin (MAP_3936), 6C9 detects electron transfer flavoprotein (MAP_3060c), and 14G11 detects MAP_3976, a lipoprotein anchoring transpeptidase. The epitopes to a selection of these mAbs were also defined. In a second approach, MAP_2698c bound monoclonal antibody (mAb) 14D4 as determined using protein arrays. When both of these approaches failed to identify the antigen for mAb 12C9, immunoprecipitation, mass spectrometry analysis, and codon optimization was used to identify the membrane protein, MAP_4145, as the reacting antigen. Characterized antibodies were used to quickly interrogate mycobacterial proteomic preps. We conclude by providing a complete catalog of available mAbs to Map proteins, along with their cognate antigens and epitopes, if known. These antibodies are now thoroughly characterized and more useful for research and diagnostic purposes.

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