Occurrence and characterization of ciprofloxacin‐resistant Escherichia coli from bovine and ovine bulk tank milk samples in Turkey

2021 ◽  
Author(s):  
Cemil Kürekci ◽  
Muhsin Aydın ◽  
İbrahim Ozan Tekeli ◽  
Pınar Ambarcıoğlu ◽  
Seydi Ahmet Şengül ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Characterization of the microbiota and resistome of bulk tank milk samples from Prince Edward Island dairy farms

2021 ◽  
Author(s):  
Landon M.C. Warder ◽  
Enrique Doster ◽  
Jennifer K. Parker ◽  
Paul S. Morley ◽  
J.T. McClure ◽  
...  
Keyword(s):  
Prince Edward Island ◽  
Dairy Farms ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Emergence of livestock-associated MRSA ST398 from bulk tank milk, China

2020 ◽  
Vol 75 (12) ◽  
pp. 3471-3474
Author(s):  
Mingquan Cui ◽  
Jiyun Li ◽  
Tariq Ali ◽  
Khisrao Kalim ◽  
Hejia Wang ◽  
...  
Keyword(s):  
Dilution Method ◽  
Whole Genome Analysis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Healthy Cattle ◽  
Genomic Characterization ◽  
Bulk Tank ◽  
Broth Dilution ◽  
Mrsa St398

Abstract Objectives To detect livestock-associated MRSA (LA-MRSA) ST398 from bulk tank milk in China and to determine the phenotypic and genomic characteristics of the strains. Methods LA-MRSA ST398 strains were isolated from bulk tank milk samples in Shanghai and their susceptibilities to antimicrobials were determined using the broth dilution method. Genomic characterization of MRSA ST398 strains was performed by WGS and their evolutionary relationships were assessed by phylogenetic analysis. Results Two LA-MRSA ST398 isolates were recovered from bulk tank milk samples in two geographically distant farms in China. Whole-genome analysis strongly suggested that the LA-MRSA ST398 strains were closely related to the highly virulent hospital-associated MRSA (HA-MRSA) ST398 strains in China. Conclusions The presence of LA-MRSA ST398 in bulk tank milk might be a serious threat to public health, highlighting the need for active surveillance of LA-MRSA in healthy cattle in China.

scholarly journals Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 841
Author(s):  
Maria Liapi ◽  
George Botsaris ◽  
Costas Arsenoglou ◽  
Nikolas Markantonis ◽  
Christodoulos Michael ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cell ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

scholarly journals Mycoplasmal mastitis in dairy cows in the Moghan region of Ardabil State, Iran : short communication

2006 ◽  
Vol 77 (4) ◽  
Author(s):  
C. Ghazaei
Keyword(s):  
Dairy Cows ◽  
Short Communication ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
The World ◽  
Bulk Tank

Mycoplasmas are an important and economically significant cause of mastitis in dairy cows in various parts of the world. The organisms are highly contagious, with the main reservoir of infection originating from cows with subclinical mastitis. In 1998 the 1st cases of bovine mastitis due to Mycoplasma bovis were diagnosed in Ardabil State, Iran. An investigation was carried out with the aim of establishing the extent of mycoplasma infections in dairy cows in Ardabil State. Milk samples obtained from 80 cows with clinical mastitis were cultured in the laboratory for the presence of mycoplasmas. Similarly, 48 bulk-tank milk samples were examined for the presence of mycoplasmas. A modified Hayflick broth was used to isolate the mycoplasmas and an immunoperoxidase test used for the species identification of the isolates. Mycoplasma bovis was isolated from 39 (48.75 %) of the clinical mastitis samples and from 48 of the bulk-tank milk samples tested. This indicated that mycoplasma udder infections were more prevalent in dairy cows in Ardabil State than previously thought.

scholarly journals Occurrence of Listeria monocytogenes and Escherichia coli in Raw Sheep's Milk from Farm Bulk Tanks in Central Italy

2020 ◽  
Vol 83 (11) ◽  
pp. 1929-1933
Author(s):  
ROBERTO CONDOLEO ◽  
GILBERTO GIANGOLINI ◽  
ALEXANDRA CHIAVERINI ◽  
DANIELA PATRIARCA ◽  
PAOLA SCARAMOZZINO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Central Italy ◽  
Risk Assessments ◽  
E Coli ◽  
Milk Samples ◽  
Related Risk ◽  
Bulk Tank ◽  
Milk Hygiene ◽  
Reported Data

ABSTRACT For milk hygiene and safety, the milking phase is a critical moment because it is a probable pathway for the introduction of unwanted microorganisms in the dairy chain. In particular, Listeria monocytogenes and Escherichia coli are known as possible microbial contaminants of raw sheep's milk, although extensive knowledge regarding their contamination dynamics on sheep farms is still lacking. This study aimed to examine the occurrence and concentration of these microorganisms in milk samples collected from farm bulk tanks in the region of Lazio (Central Italy) and to investigate the related risk factors. Over a period of 1 year, we collected 372 milk samples from 87 sheep farms and administered a questionnaire to acquire information regarding relevant farm management variables. L. monocytogenes was not found in any of the samples, which indicates a low occurrence of this pathogen in sheep's bulk tank milk. In contrast, E. coli was found in almost two-thirds of milk samples (61%) but at levels below 102 CFU/mL in most of them (approximately 75%). Statistical analysis indicated that, during the warmest seasons, E. coli presence is more probable and counts are significantly higher. Unexpectedly, milk collected by hand milking had a lower level of contamination. Although further studies are necessary to clarify some aspects, the reported data add to the knowledge about the occurrence of L. monocytogenes and E. coli in raw sheep's milk and will be useful for future risk assessments. HIGHLIGHTS

scholarly journals Whole-Genome Epidemiology and Characterization of Methicillin-Susceptible Staphylococcus aureus ST398 From Retail Pork and Bulk Tank Milk in Shandong, China

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaonan Zhao ◽  
Ming Hu ◽  
Cui Zhao ◽  
Qing Zhang ◽  
Lulu Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Whole Genome ◽  
Staphylococcal Protein A ◽  
Sequencing Analysis ◽  
Bulk Tank Milk ◽  
Predominant Type ◽  
Bulk Tank ◽  
The One

Staphylococcus aureus (S. aureus) is now regarded as a zoonotic agent. Methicillin-susceptible S. aureus (MSSA) ST398 is a livestock-associated bacterium that is most prevalent in China, but there are currently no data available for Shandong. Therefore, the aim of this study was to investigate the epidemiology and characterization of MSSA ST398 from retail pork and bulk tank milk (BTM) in Shandong. A total of 67 S. aureus isolates were collected from retail pork between November 2017 and June 2018. Among the isolates, high antimicrobial resistance rates were observed for penicillin (97.0%), and 92.5% of the isolates were multi-drug resistant (MDR). Eight sequence types (STs) were identified in the retail pork isolates, and the predominant type was ST15 (n=26), which was followed by ST398 (n=14). Staphylococcal protein A gene (spa) typing identified spa types t034 and t1255 in MSSA ST398 from retail pork. Using whole-genome sequencing analysis, we described the phylogeny of 29 MSSA ST398 isolates that were obtained from retail pork (n=14) and BTM (n=15). The phylogenetic tree showed that the MSSA ST398 isolates from different sources had the same lineage. Among the 29 MSSA ST398 isolates, five resistance genes were detected, and all isolates carried DHA-1. Fifteen toxin genes were detected, and all isolates carried eta, hla, and hlb. In conclusion, this study found that a high risk for MSSA ST398 was present in retail pork and BTM. These findings have major implications for how investigations of MSSA ST398 outbreaks should be conducted in the One-Health context.

Performance of Various Tests Used to Screen Antibiotic Residues in Milk Samples from Individual Animals

1994 ◽  
Vol 77 (4) ◽  
pp. 862-870 ◽  
Author(s):  
James S Cullor ◽  
Alison Van Eenennaam ◽  
Ian Gardner ◽  
Lynn Perani ◽  
Jon Dellbvger ◽  
...  
Keyword(s):  
False Positive ◽  
Dairy Industry ◽  
Initial Therapy ◽  
Treatment Withdrawal ◽  
Quality Assurance Program ◽  
Antibiotic Residues ◽  
Withdrawal Time ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Abstract The 10-point Milk and Dairy Beef Quality Assurance Program was developed collaboratively by the National Milk Producers Federation and the American Veterinary Medical Association and is designed to promote and document the responsible use of antibiotics in the dairy industry. One area of emphasis in this program is testing of individual animals for antibiotic residues after a specified post-treatment withdrawal time. We examined the performance of various assay systems on milk samples from individual cows. These assays are used at present on bulk tank milk samples by regulatory agencies, processing plants, producers, and veterinarians to detect the presence of β-lactam antibiotics. A high proportion of false-positive results was obtained for both the pretreatment milk samples from cows with clinical mastitis and the milk samples obtained 21 days after initial therapy (nonantibiotic and antibiotic) for the treatment of mastitis. A high proportion of false-positive outcomes was obtained from the milk of clinically normal cows that had not received any medication for at least 30 days prior to evaluation. The results indicate a serious problem in the use of some assays that were designed to evaluate residues bulk tank milk samples to analyze samples from individual cows. This error in assay specificity results in the unjustifiable discarding of milk that meets regulatory standards and may be misused to accuse the producer or veterinarian of not adhering to regulatory guidelines. Maintaining a safe, high-quality milk supply is a constant goal of the dairy industry, which must be provided the appropriate tools and techniques to meet this challenge.

Evaluation of the Delvo-X-Press Assay for Detecting Antibiotic Residues in Milk Samples from Individual Cows

1999 ◽  
Vol 62 (10) ◽  
pp. 1183-1190 ◽  
Author(s):  
APOSTOLOS S. ANGELIDIS ◽  
THOMAS B. FARVER ◽  
JAMES S. CULLOR
Keyword(s):  
False Positive ◽  
Bovine Lactoferrin ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Lactating Cows ◽  
Bovine Plasma ◽  
Positive Linear Correlation ◽  
Lactam Antibiotic ◽  
Bulk Tank ◽  
Positive Results

Performance of the Delvo-X-Press β-lactam antibiotic assay was examined using bulk-tank milk samples and milk samples from individual cows. Bulk-tank milk samples fortified with bovine lactoferrin at a concentration of 1 mg/ml or more consistently tested positive. False-positive results were also obtained from bulk-tank milk samples fortified with bovine plasma at concentrations of 20 and 40%. The assay yielded positive results for milk with antibiotic concentrations as low as 2 ppb. Individual milk samples were collected from 144 healthy lactating cows and from 34 cows with chronic Staphylococcus aureus mastitis. Specificity estimates for samples from healthy and mastitic cows were 0.88 (95% confidence interval [CI], 0.82, 0.93) and 0.94 (95% CI, 0.86, 1.00), respectively. Individual milk samples were collected from three cows with experimentally induced mastitis for 21 consecutive days. False-positive results occurred as late as 12 days postchallenge. A moderate but significant (P < 0.01) positive linear correlation (r = 0.61) was observed between test result and somatic cell count (SCC) values in milk samples with SCCs of >106/ml.

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