Reference intervals for coagulation times using two point-of-care analysers in healthy pet rabbits (Oryctolagus cuniculus)

2014 ◽  
Vol 174 (26) ◽  
pp. 658-658 ◽  
Author(s):  
V. Mentré ◽  
C. Bulliot ◽  
A. Linsart ◽  
P. Ronot
Keyword(s):  
Oryctolagus Cuniculus ◽  
Point Of Care ◽  
Reference Intervals

Comparison of serum and plasma SDMA measured with point-of-care and reference laboratory analysers: implications for interpretation of SDMA in cats

2021 ◽  
pp. 1098612X2098326
Author(s):  
Randolph M Baral ◽  
Kathleen P Freeman ◽  
Bente Flatland
Keyword(s):  
Point Of Care ◽  
Total Error ◽  
Clinical Results ◽  
Reference Intervals ◽  
Clinical Samples ◽  
Performance Goals ◽  
Specific Reference ◽  
Reference Laboratory ◽  
Laboratory Equipment ◽  
Commercial Laboratory

Objectives Symmetric dimethylarginine (SDMA) reflects the glomerular filtration rate (GFR) in people, dogs and cats. Initial assays used a liquid chromatography-mass spectroscopy (LC-MS) technique. A veterinary immunoassay has been developed for use in commercial laboratories and point-of-care (POC) laboratory equipment. This study sought to: determine POC and commercial laboratory (CL) SDMA assay imprecision; determine any bias of the POC assay compared with the CL assay; calculate observed total error of the POC assay and compare with analytical performance goals; and calculate dispersion and sigma metrics (σ) for POC and CL SDMA methods. Methods Two separate studies were performed that assessed: (1) imprecision, determined by evaluation of pooled feline plasma or serum; and (2) bias, assessed by comparing pooled plasma and serum results, as well as paired analyses of clinical samples from a single venepuncture measured using both analysers. Results were assessed in relation to performance goals. Dispersion and σ were calculated for both analysers. Results Bias between CL and POC analysers was consistent and high numbers of clinical results were outside performance goals across both studies. Imprecision was poor for both analysers for study 1 and improved to within quality goals for the CL analyser for study 2. Dispersion was at least 40%, meaning a measured result of 14 μg/dl represents a range of possible results from 8 μg/dl to 20 μg/dl. Conclusions and relevance Clinicians should be careful ascribing medical significance to small changes in SDMA concentration, as these may reflect analytical and biological variability. Analyser-specific reference intervals are likely required.

Blood reference intervals for rabbits (Oryctolagus cuniculus) from routine diagnostic samples

2018 ◽  
Vol 46 (06) ◽  
pp. 393-398 ◽  
Author(s):  
Christoph Leineweber ◽  
Elisabeth Müller ◽  
Rachel E. Marschang
Keyword(s):  
Oryctolagus Cuniculus ◽  
Clinical Chemistry ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
Reference Intervals ◽  
Specific Reference ◽  
Data Sets ◽  
Reference Limit ◽  
Statistical Program ◽  
Diagnostic Samples

Summary Objective: The goal of this study was to establish reference intervals for blood parameters in rabbits based on data from routine diagnostic samples. Materials and methods: Data sets from 1679–2039 values for clinical chemistry and 937–1559 values for hematological parameters were evaluated using the statistical program Reference Limit Estimator 20151017. Results: The following reference intervals were calculated for hematology: erythrocytes 4.37–7.43 × 1012 cells/l; hematocrit 0.28–0.48 l/l; hemoglobin 89.63–153.82 g/l; leucocytes 2.71–12.23 × 109 cells/l; neutrophils 0.87–7.82 × 109 cells/l; lymphocytes 0.36–6.58 × 109 cells/l; monocytes 0.08–1.71 × 109 cells/l; eosi nophils 0.07–0.19 × 109 cells/l; basophils 0.06–1.1 × 109 cells/l; thrombocytes 225.45–905.3 × 109 cells/l. Calculated intervals for clinical chemistries were: alkaline phosphatase 9.05–94.68 U/l; aspartataminotransferase 3.75–32.44 U/l; creatine kinase 1.63–559.53 U/l; γ-glutamyltransferase 2.5–14.46 U/l; glutamatdehydrogenase 0.68–14.78 U/l; fructosamin 248.08–501.43 µmol/l; bile acid 0.76–19.63 μmol/l; total protein 48.66–73.64 g/l; urea 2.63–10.28 mmol/l; creatinine 51.38–154.35 µmol/l; calcium 3.02–4.3 mmol/l; magnesium 0.66–1.51 mmol/l; phosphorus 0.54–2.18 mmol/l; sodium 132.61– 154.0 mmol/l and potassium 3.52–6.04 mmol/l. Conclusion and clinical relevance: The calculation of intervals based on a large number of routine diagnostic samples allows the establishment of labora tory specific reference intervals without the use of experimental animals.

scholarly journals Whole blood platelet impedance aggregometry with the ROTEM platelet device: comparison of 2 anticoagulants and storage times for the establishment of canine reference intervals

2021 ◽  
pp. 104063872110513
Author(s):  
Lara M. Heimgartner ◽  
Martina Stirn ◽  
Annette P. N. Kutter ◽  
Nadja E. Sigrist ◽  
Rahel Jud Schefer
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Blood Platelet ◽  
Reference Intervals ◽  
Sample Storage ◽  
Rotational Thromboelastometry ◽  
Blood Samples ◽  
Impedance Aggregometry ◽  
Storage Times ◽  
And Storage

The ROTEM platelet device, a point-of-care whole blood platelet impedance aggregometer, is an add-on to the rotational thromboelastometry ROTEM delta device. The latter has been validated in dogs. We examined whether canine whole blood is suited for analysis with the ROTEM platelet device using adenosine-5′-diphosphate (ADP) and arachidonic acid (ARA) as agonists for platelet activation, and if there are significant differences between sample storage times and anticoagulants used. Subsequently, we determined canine reference intervals (RIs) for the ROTEM platelet device for ADP and ARA. In a pilot study, we examined whole blood from 7 dogs after 15-min and 60-min storage of lithium-heparinized samples and 40-min and 80-min storage of hirudinized samples. Statistical analysis showed no significant differences between ROTEM platelet device results for both ADP and ARA in lithium-heparin and hirudin anticoagulated canine whole blood. Lithium-heparinized blood samples analyzed after 15-min storage had the lowest coefficient of variation. RIs were determined for heparinized whole blood samples from 49 dogs after 15 min of storage.

scholarly journals Organizational challenges in the management of point-of-care diagnostics in healthcare facilities

2020 ◽  
Vol 44 (2) ◽  
pp. 103-105 ◽  
Author(s):  
Marco Kachler ◽  
Christiane Maschek
Keyword(s):  
Best Practice ◽  
Point Of Care ◽  
Reference Intervals ◽  
Full Understanding ◽  
Healthcare Facilities ◽  
Organizational Challenges ◽  
Point Of Care Diagnostics ◽  
Competence Management ◽  
English Speaking ◽  
Practice Implementation

AbstractThis text is a synopsis of the “Seminar on Organizational Aspects of POCT Management” which was part of the 4th Munich POCT symposium 2019. The session was chaired in part 1 by Christiane Maschek (Berlin) and Anke Urban (Ludwigshafen), and in part 2 by Barbara Oschwald-Häg (Offenburg) and Marco Kachler (Klagenfurt/AT). The seminar was held in German in order to allow non-English-speaking medical technicians the full understanding of the presented contents. Part 1: Nice to Know – Challenges for networking POCT systems (Michaela Markhoff, Hamburg). Best Practice – Implementation of POCT in a hospital without central lab services (Jennifer Planz, Essen). Best Practice – Benefits of the POCT commission demonstrated using the example of implementing glucose POCT devices (Barbara Oschwald-Häg, Offenburg). Part 2: Best Practice – Challenges of a group-wide implementation of a POCT competence management (Sandra Mütze, Berlin). Nice to Know – Learning from mistakes in preanalytics (Reno Konzack, Berlin). New IFCC recommendation for checking reference intervals (Harald Maier, Altötting).

scholarly journals Establishment of reference intervals for Platelet Function Analyzer -100 closure time in Algerian adults

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249402
Author(s):  
Malika Belkacemi ◽  
Yassine Merad ◽  
Mohamed Amine Merbouh
Keyword(s):  
Platelet Function ◽  
Clinical Chemistry ◽  
Point Of Care ◽  
Reference Intervals ◽  
Primary Hemostasis ◽  
Asian Populations ◽  
Closure Time ◽  
Function Analyzer ◽  
Time Required ◽  
Platelet Function Analyzer

Introduction The Platelet Function Analyzer-100 (PFA-100) is a point of care instrument that simulates plug formation under high shear flow. The PFA-100 measures the time required to occlude the aperture in a biochemically active cartridge and is expressed in a term of closure time (CT). In Algeria, the reference values used in clinical laboratories are of Western origin. However, ethnic, genetic, dietary environmental, and diet differences between populations may affect reference intervals. We established the reference intervals of PFA-100 closure times in healthy Algerian adults according to the International Federation of Clinical Chemistry method, and we compared them with those of Western and Asian countries. Material and methods We enrolled 303 healthy blood donors in the study. 218 subjects met inclusion criteria. We analyzed the blood sample on the PFA-100 for CT with both the collagen epinephrine and collagen ADP cartridges. Results The reference intervals of PFA-100 collagen epinephrine CT and PFA-100 collagen ADP CT were 91–207 seconds and 71–144 seconds, respectively. Compared to Western and Asian populations, there were significant differences. The upper limits of CTs were higher for Algerians in this study. Our findings show that many healthy Algerians would be incorrectly identified as having a primary hemostasis abnormality according to the reference intervals of the manufacturer and scientific literature. Conclusion This report provides the first reference intervals for PFA-100 CTs in healthy Algerian adults. These results improve the accuracy of diagnosis and patient care in Algeria.

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