scholarly journals Molecular biology and respiratory disease. I-- Basic principles.

Thorax ◽  
1990 ◽  
Vol 45 (1) ◽  
pp. 52-56
Author(s):  
C A Owen ◽  
R A Stockley
Keyword(s):  
Molecular Biology ◽  
Respiratory Disease ◽  
Basic Principles

Molecular Biology of PCR Testing for COVID-19 Diagnostics

2021 ◽  
Author(s):  
Vinita Chittoor-Vinod
Keyword(s):  
Molecular Biology ◽  
Respiratory Disease ◽  
Diagnostic Tests ◽  
Genetic Material ◽  
Molecular Diagnostic ◽  
Basic Principles ◽  
Asymptomatic Carriers ◽  
Diagnostic Assays ◽  
Global Pandemic ◽  
Asymptomatic Individuals

COVID-19 cases were first reported in December 2019, and since then it has spread quickly to create a global pandemic. This respiratory disease is caused by the SARS-CoV-2 virus. A major contributing factor for the fast spread of this virus is that the infectivity by the asymptomatic carriers is similar to symptomatic patients. Thus, to identify the asymptomatic individuals and to provide the essential treatment and care to COVID-19 patients, we rely heavily on diagnostic assays. Efficient, reproducible and accessible diagnostic tests are crucial in combatting a pandemic. Currently, there are few key detection tests which have been successfully employed to field-use. However, there are constant efforts to enhance their efficacy and accessibility. This chapter aims at explaining the basic principles of the current molecular diagnostic tests, which determine the presence of the virus through the detection of its genetic material. This chapter will aid the readers in understanding the basic workings of these molecular diagnostic tests.

scholarly journals Three basic types of fluorescence microscopy and recent improvement

2021 ◽  
Vol 290 ◽  
pp. 01031
Author(s):  
Xinyi Wang ◽  
Yunyan Lai
Keyword(s):  
Confocal Microscopy ◽  
Molecular Biology ◽  
Fluorescence Microscopy ◽  
Spatial Resolution ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Future Perspectives ◽  
Recent Improvement ◽  
Basic Principles

Fluorescence microscopy is one of the most used imaging modalities in molecular biology and living specimens. To increase image contrast and spatial resolution, different type of fluorescence microscopy has been developed. This review introduces three main types of fluorescence microscopy: wild-field microscopy, confocal microscopy, and total internal reflection fluorescence microscopy. The basic principles are similar but with different modifications, which also indicates their attributes and limitation. The recent improvement on these microscopies is also discussed. Some most recent techniques show advance in overcoming common fluorescence microscopy's weakness, and future perspectives are also discussed.

Recent advances in biological imaging

1989 ◽  
Vol 9 (4) ◽  
pp. 437-449 ◽  
Author(s):  
Helen Saibil ◽  
Nicholas White
Keyword(s):  
Electron Microscopy ◽  
Image Processing ◽  
Molecular Biology ◽  
Digital Image Processing ◽  
Digital Image ◽  
Light And Electron Microscopy ◽  
Biological Imaging ◽  
Basic Principles ◽  
New Methods ◽  
Recent Advances

Recent innovations in microscopy and digital image processing have greatly enhanced the power of biological imaging. Basic principles of several new methods in light and electron microscopy will be discussed, and examples presented of their application to cell and molecular biology.

NANOTECHNOLOGY AND HUMAN DISEASES

COSMOS ◽  
2007 ◽  
Vol 03 (01) ◽  
pp. 89-101 ◽  
Author(s):  
GABRIEL YEW HOE LEE ◽  
CHWEE TECK LIM
Keyword(s):  
Mechanical Properties ◽  
Molecular Biology ◽  
Disease Progression ◽  
Viscoelastic Properties ◽  
State Of The Art ◽  
Living Cells ◽  
Molecular Structures ◽  
Human Diseases ◽  
Diagnosis And Treatment ◽  
Basic Principles

Tissues, cells and biomolecules can experience changes in their structural and mechanical properties during the occurrence of certain diseases. Recent advances in the fields of nanotechnology, biomechanics and cell and molecular biology have led to the development of state-of-the-art and novel biophysical and nanotechnological tools to probe the mechanical properties of individual living cells and biomolecules. Here we will review the basic principles and application of some of these nanotechnological tools used to relate changes in the elastic and viscoelastic properties of cells to alterations in the cellular and molecular structures induced by diseases such as malaria and cancer. Knowing the ways and the extent to which mechanical properties of living cells are altered with the onset of disease progression will be crucial for us to gain vital insights into the pathogenesis and pathophysiology of malaria and cancer, and potentially offers the opportunity to develop new and better methods of detection, diagnosis and treatment.

Sign in / Sign up

Export Citation Format

Share Document