Recent advances in biological imaging

1989 ◽  
Vol 9 (4) ◽  
pp. 437-449 ◽  
Author(s):  
Helen Saibil ◽  
Nicholas White

Recent innovations in microscopy and digital image processing have greatly enhanced the power of biological imaging. Basic principles of several new methods in light and electron microscopy will be discussed, and examples presented of their application to cell and molecular biology.

Author(s):  
K. N. Colonna ◽  
G. Oliphant

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.


1988 ◽  
Vol 107 (2) ◽  
pp. 521-530 ◽  
Author(s):  
W. Coene ◽  
A. F. de Jong ◽  
D. van Dyck ◽  
G. van Tendeloo ◽  
J. van Landuyt

2018 ◽  
Vol 141 ◽  
pp. 395-406 ◽  
Author(s):  
Andrew Campbell ◽  
Paul Murray ◽  
Evgenia Yakushina ◽  
Stephen Marshall ◽  
William Ion

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