scholarly journals Reverse passive haemagglutination test for the rapid identification of Neisseria gonorrhoeae and detection of penicillinase production.

1979 ◽  
Vol 55 (6) ◽  
pp. 404-407
Author(s):  
R Munro ◽  
R Mallon
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Rapid Identification ◽  
Passive Haemagglutination ◽  
Haemagglutination Test

Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory

1976 ◽  
Vol 4 (1) ◽  
pp. 19-21
Author(s):  
H M Pollock
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Laboratory ◽  
Fluorescent Antibody ◽  
Rapid Identification ◽  
Reliable Identification ◽  
Initial Trial ◽  
Carbohydrate Degradation ◽  
Routine Clinical Laboratory ◽  
Evaluation Of Methods ◽  
Produce Acid

Of 78 isolates of Neisseria gonorrhoeae, 21 failed to grow and produce acid in unsupplemented cystine-Trypticase agar (CTA); whereas positive reactions were obtained by using serum-supplemented CTA and fluorescent antibody (FA). An additional 290 strains of Neisseria were evaluated by FA and by a rapid carbohydrate degradation technique (RF). There was agreement between the two methods 92% of the time on the initial trial and 99% of the time with repeats on discrepancies. The RF and FA tests provided rapid and reliable identification of N. gonorrhoeae, alleviating the problems of CTA due to lack of growth and need for overnight incubation.

scholarly journals Passive haemagglutination test for human neurocysticercosis immunodiagnosis: I. Standardization and evaluation of the passive haemagglutination test for the detection of anti-Cysticercus cellulosae antibodies

1988 ◽  
Vol 30 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Mirthes Ueda ◽  
Eide Dias Camargo ◽  
Adelaide José Vaz ◽  
Ana Maria Carvalho de Souza ◽  
Regina Maria Figueiredo ◽  
...  
Keyword(s):  
Treated Group ◽  
Control Group ◽  
Predictive Values ◽  
Saline Extract ◽  
Passive Haemagglutination ◽  
Specific Antibodies ◽  
Haemagglutination Test ◽  
Cysticercus Cellulosae ◽  
The Mean ◽  
Human Red Cells

A passive haemagglutination test (PHA) for human neurocysticercosis was standardized and evaluated for the detection of specific antibodies to Cysticercus cellulosae in cerebrospinal fluid (CSF). For the assay, formaldehyde-treated group O Rh-human red cells coated with the cysticerci crude total saline extract (TS) antigen were employed. A total of 115 CSF samples from patients with neurocysticercosis was analysed, of these 94 presented reactivity, corresponding to 81.7% sensitivity, in which confidence limit of 95% probability (CL95%) ranged from 74.5% to 88.9%. Eighty-nine CSF samples derived from individuals of control group presented as nonreactive in 94.4% (CL95% from 89.6% to 99.2%). The positive and negative predictive values were 1.4% and 99.9%, respectively, considering the mean rate of that this assay provide a rapid, highly reproducible, and moderately sensitive mean of detecting specific antibodies in CSF samples.

Reverse passive haemagglutination test in the diagnosis of infectious bursal disease

1995 ◽  
Vol 27 (1) ◽  
pp. 43-46 ◽  
Author(s):  
K. Nachimuthu ◽  
G. Dhinakar Raj ◽  
A. Thangavelu ◽  
R. A. Venkatesan
Keyword(s):  
Infectious Bursal Disease ◽  
Passive Haemagglutination ◽  
Haemagglutination Test ◽  
Bursal Disease

The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. II. Use of hen antiserum to gonococcal lipopolysaccharide in a rapid slide test for the identification of N. gonorrhoeae from primary isolates and secondary cultures

1978 ◽  
Vol 24 (2) ◽  
pp. 124-128 ◽  
Author(s):  
R. Wallace ◽  
F. E. Ashton ◽  
A. Ryan ◽  
B. B. Diena ◽  
C. Malysheff ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Rapid Identification ◽  
Cross Reactivity ◽  
Agglutination Test ◽  
Common Antigen ◽  
Colony Type ◽  
Neisseria Lactamica ◽  
Slide Agglutination Test ◽  
Slide Test ◽  
Branhamella Catarrhalis

An antiserum has been prepared in hens to R-type gonococcal lipopolysaccharide (LPS) and used in a simple slide-agglutination test for the identification of Neisseria gonorrhoeae. Anti-LPS serum agglutinated gonococcal cells representative of the four colony types of N. gonorrhoeae. Absorption of the antiserum with LPS removed the agglutinating activity. Secondary cultures (1120) were tested without observation of the colony type and all were agglutinated. No agglutination occurred with strains of Neisseria meningitidis, Neisseria lactamica, non-pathogenic Neisseria, Pseudomonas aeruginosa, Branhamella catarrhalis, or with species of lactobacilli and Acinetobacter. Cross-reactivity of the antiserum occurred with some streptococci. The anti-LPS serum was used to identify N. gonorrhoeae in primary isolates from the cervix, urethra, and pharynx. Of 251 gonococcal isolates tested, 249 were agglutinated by the antiserum, while all of the corresponding secondary cultures were agglutinated. The antiserum did not agglutinate N. meningitidis found in primary isolates from pharyngeal specimens. Anti-LPS hen serum should be useful for the rapid identification of N. gonorrhoeae in primary isolates or secondary cultures.

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