The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. II. Use of hen antiserum to gonococcal lipopolysaccharide in a rapid slide test for the identification of N. gonorrhoeae from primary isolates and secondary cultures

1978 ◽  
Vol 24 (2) ◽  
pp. 124-128 ◽  
Author(s):  
R. Wallace ◽  
F. E. Ashton ◽  
A. Ryan ◽  
B. B. Diena ◽  
C. Malysheff ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Rapid Identification ◽  
Cross Reactivity ◽  
Agglutination Test ◽  
Common Antigen ◽  
Colony Type ◽  
Neisseria Lactamica ◽  
Slide Agglutination Test ◽  
Slide Test ◽  
Branhamella Catarrhalis

An antiserum has been prepared in hens to R-type gonococcal lipopolysaccharide (LPS) and used in a simple slide-agglutination test for the identification of Neisseria gonorrhoeae. Anti-LPS serum agglutinated gonococcal cells representative of the four colony types of N. gonorrhoeae. Absorption of the antiserum with LPS removed the agglutinating activity. Secondary cultures (1120) were tested without observation of the colony type and all were agglutinated. No agglutination occurred with strains of Neisseria meningitidis, Neisseria lactamica, non-pathogenic Neisseria, Pseudomonas aeruginosa, Branhamella catarrhalis, or with species of lactobacilli and Acinetobacter. Cross-reactivity of the antiserum occurred with some streptococci. The anti-LPS serum was used to identify N. gonorrhoeae in primary isolates from the cervix, urethra, and pharynx. Of 251 gonococcal isolates tested, 249 were agglutinated by the antiserum, while all of the corresponding secondary cultures were agglutinated. The antiserum did not agglutinate N. meningitidis found in primary isolates from pharyngeal specimens. Anti-LPS hen serum should be useful for the rapid identification of N. gonorrhoeae in primary isolates or secondary cultures.

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals Identification of Neisseria gonorrhoeae from primary cultures by a slide agglutination test

1978 ◽  
Vol 8 (2) ◽  
pp. 260-261
Author(s):  
C Malysheff ◽  
R Wallace ◽  
F E Ashton ◽  
B B Diena ◽  
M B Perry
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Primary Cultures ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Slide Agglutination Test

Hen antigonococcal lipopolysaccharide hen serum was used in a simple slide agglutination test for the identification of Neisseria gonorrhoeae from primary isolates.

scholarly journals COMPARISON OF QUANTITATIVE TUBE AGGLUTINATION AND SEMIQUANTITATIVE SLIDE AGGLUTINATION TEST FOR DIAGNOSING OF THE SUSPECTED CASES OF ENTERIC FEVER

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Shubhdeep Kaur ◽  
Harsh Yadav ◽  
Umed Singh ◽  
Mona Narain ◽  
Mayank Bhardwaj ◽  
...  
Keyword(s):  
Predictive Value ◽  
Enteric Fever ◽  
Venous Blood ◽  
Laboratory Method ◽  
Agglutination Test ◽  
Major Health Problem ◽  
Slide Agglutination ◽  
Slide Agglutination Test ◽  
Slide Test ◽  
Sensitivity Specificity

Introduction: Enteric fever is the major health problem of developing country like India, with a notable morbidity and mortality. Isolation of Salmonella the causative agent from Blood is the standard laboratory method for diagnosis, but it is not available at PHC level. So, rapid and affordable diagnostic test like Widal tube and slide agglutination test are used. The present study was done to comparatively evaluate the Widal slide agglutination and tube agglutination test in detecting enteric fever. Methods: A total of 500 patients with clinical presentation suggestive of enteric fever were included in the study whose venous blood was collected. All the samples were tested for the presence of anti O and anti H agglutinins against S. typhi and S. paratyphi A by semi quantitative slide and quantitative tube agglutination tests as per standard protocols. The titers of 1:80 (O agglutinins) and 1:160 (H agglutinins) were taken as the significant titer for the diagnosis of enteric fever. Results: Out of 500 collected samples, 183 (36.6%) was positive by slide agglutination test, whereas, only 145 (29%) were positive by tube agglutination method. The slide test had a sensitivity of 97.2%, specificity of 88.1%, positive predictive value of 77% and negative predictive value of 98.7% as compared to Widal tube agglutination test. Conclusions: Due to high false positivity shown by slide test, it is suggested that serological diagnosis should not be made solely on the basis of slide test rather its results should be confirmed by using Widal tube agglutination test. Keywords: Enteric fever, Slide agglutination test, Widal tube agglutination test, Sensitivity, Specificity

Induction of reaginic (IgE) gonococcal antibodies in the rat by a common antigen of Neisseria gonorrhoeae

1979 ◽  
Vol 25 (2) ◽  
pp. 138-145
Author(s):  
F. E. Ashton ◽  
H. M. Vijay ◽  
G. Lavergne ◽  
B. R. Brodeur ◽  
B. B. Diena
Keyword(s):  
Molecular Weight ◽  
Neisseria Gonorrhoeae ◽  
Cross Reactivity ◽  
Low Molecular Weight ◽  
Common Antigen ◽  
Rat Serum ◽  
Ige Antibody ◽  
Antigenic Activity ◽  
Diethylaminoethyl Cellulose ◽  
The Common

An antigen (ZAB) common to Neisseria gonorrhoeae was prepared by stepwise elution of a crude gonococcal antigen (ZA) from columns of diethylaminoethyl cellulose employing 0.02 M phosphate buffers, pH 7.6, containing increasing concentrations of sodium chloride. Rats immunized with ZAB produced reaginic (IgE) antibody which cross-reacted with ZA prepared from eight gonococcal strains by the passive cutaneous anaphylaxis (PCA) test. Heatingof the sera at 56 °C for 4 h destroyed the PCA activity. The PCA activity of the anti-ZAB rat serum was removed after absorption with ZAB antigen or with rabbit anti-rat IgE but not after absorption with gonococcal lipopolysaccharide or with heat-killed or formalinized gonococci. Treatment of ZAB with trypsin or heating at 100 °C for 30 min destroyed or reduced the antigenic activity respectively. Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven strains of N. gonorrhoeae. Fraction ZAB2 contained material which had a molecular weight less than 13 700 and was associated with the presence of material absorbing at 260 nm. The results of this study indicate that a low molecular weight antigen, which appears to be protein in nature and associated with nucleic acid, is common to the gonococcus and is the main antigenic component inducing reaginic (IgE) antibody in the rat.

The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. I. Immunizing properties

1978 ◽  
Vol 24 (2) ◽  
pp. 117-123 ◽  
Author(s):  
B. B. Diena ◽  
F. E. Ashton ◽  
A. Ryan ◽  
R. Wallace ◽  
M. B. Perry
Keyword(s):  
Animal Model ◽  
Neisseria Gonorrhoeae ◽  
Chicken Embryo ◽  
Common Antigen ◽  
Colony Type ◽  
Chemically Modified

The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoeae colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 μg of LPS, and challenged intracerebrally with 10–20 LD50's of N. gonorrhoeae colony type 1. Immunized mice were significantly protected (P < 0.01 to < 0.05) against challenge with different T1 isolates of N. gonorrhoeae when compared with non-immunized mice. Mice, injected with succinylated or alkali-treated LPS were not protected against gonococcal challenges.In a second animal model, leghorn hens were immunized intravenously with three injections of 500 μg of LPS followed by a booster of 2.5 mg 2 weeks later. Embryonated eggs obtained from immunized hens were protected against challenge with 5 × 103 – 1 × 104 LD50's of three different T1 isolates. When hens were injected with the chemically modified LPS, the embryos were not resistant to gonococcal challenge. The results of this study demonstrate the ability of R-type gonococcal LPS to provide protection against different T1 isolates of N. gonorrhoeae.

scholarly journals Evaluation of an indirect hemagglutination test for the diagnosis of human leptospirosis

1975 ◽  
Vol 2 (3) ◽  
pp. 218-221
Author(s):  
C R Sulzer ◽  
J W Glosser ◽  
F Rogers ◽  
W L Jones ◽  
M Frix
Keyword(s):  
Agglutination Test ◽  
Serological Diagnosis ◽  
Alcohol Extract ◽  
Slide Agglutination ◽  
Human Leptospirosis ◽  
Hemagglutination Test ◽  
Slide Agglutination Test ◽  
Indirect Hemagglutination ◽  
Slide Test ◽  
Indirect Hemagglutination Test

A presumptive hemagglutination test for the serological diagnosis of leptospirosis in humans is described. The antigen was prepared from a soluble alcohol extract of an andamana strain sorbed to human O-negative erythrocytes and preserved by pyruvic aldehyde fixation. In this study, the overall sensitivity of the hemagglutination test was 92% in contrast to 69% for the presumptive slide agglutination test. The specificity was 95% for the hemagglutination test in comparison with 83% for the slide test.

scholarly journals Serological classification of Pseudomonas aeruginosa by a slide agglutination test

1978 ◽  
Vol 8 (2) ◽  
pp. 181-188
Author(s):  
H Kusama
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Expert Panel ◽  
International Committee ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serological Classification ◽  
Slide Agglutination Test ◽  
Slide Test ◽  
O Antigens

Serological classification of Pseudomonas aeruginosa by the slide agglutination test with live organisms was studied, based on the O antigen schema adopted by the international expert panel sponsored by the Subcommittee on Pseudomonas and Related Organisms of the International Committee on Systematic Bacteriology. The typing results obtained by the slide test with well-absorbed O sera were identical to those obtained by the conventional tube agglutination test with autoclaved organisms. Most O antigens occur singly; but O2, O5, and O16 occur in four combinations. Antigens O13 and O14 are closely related, as are O7 and O8, and it would be convenient to classify organisms possessing these antigens collectively as O7,8 and O13,14.

The Lipopolysaccharides of Neisseria gonorrhoeae Colony Types 1 and 4

1975 ◽  
Vol 53 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Malcolm B. Perry ◽  
Virginia Daoust ◽  
Benito B. Diena ◽  
Fraser E. Ashton ◽  
Rebecca Wallace
Keyword(s):  
Molecular Weight ◽  
Neisseria Gonorrhoeae ◽  
High Molecular Weight ◽  
Lipid A ◽  
Core Oligosaccharide ◽  
Colony Type ◽  
Mild Hydrolysis

The lipopolysaccharides (LPS) of strains of Neisseria gonorrhoeae grown in type 1 (T1) and 4 (T4) colony forms have been isolated. LPS from T4 colony type cells on mild hydrolysis gave a lipid A and a core oligosaccharide composed of 2-amino-2-deoxy-D-glucose, D-glucose, D-galactose, L-glycero-D-manno-heptose and 3-deoxy-D-manno-octulosonic acid that appeared to be common to all the strains examined. LPS from T1 colony type cells on mild hydrolysis gave a lipid A and high molecular weight O polysaccharides which showed considerable differences in glycose composition for each strain examined. In those strains examined, T4 cells appear to produce a common 'R' type LPS whereas T1 cells produce an 'S' type LPS with structurally different O polysaccharide structures which probably account for serologically differentiated strains of N. gonorrhoeae.

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