scholarly journals Use of electron microscopy in examination of faeces and rectal and jejunal biopsy specimens.

1991 ◽  
Vol 44 (4) ◽  
pp. 313-316 ◽  
Author(s):  
G M Connolly ◽  
D S Ellis ◽  
J E Williams ◽  
G Tovey ◽  
B G Gazzard
1979 ◽  
Vol 57 (2) ◽  
pp. 181-185 ◽  
Author(s):  
B. T. Cooper ◽  
D. C. A. Candy ◽  
J. T. Harries ◽  
T. J. Peters

1. Jejunal biopsy specimens from three children with congenital sucrase—isomaltase deficiency were assayed for disaccharidase activity and were subjected to analytical subcellular fractionation with enzymic microanalysis. 2. By use of the highly sensitive fluorigenic modification of the disaccharidase assay, brush-border sucrase and isomaltase activities were depressed but nevertheless detectable in each child. 3. Apart from the expected decrease in brush-border α-glucosidase activity, the other enterocyte marker-enzyme activities were normal. 4. There were no abnormalities in the enterocytes of any child on analytical subcellular fractionation or on electron microscopy.


Author(s):  
John H. L. Watson ◽  
C. N. Sun

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.


Author(s):  
John M. Basgen ◽  
Eileen N. Ellis ◽  
S. Michael Mauer ◽  
Michael W. Steffes

To determine the efficiency of methods of quantitation of the volume density of components within kidney biopsies, techniques involving a semi-automatic digitizing tablet and stereological point counting were compared.Volume density (Vv) is a parameter reflecting the volume of a component to the volume that contains the component, e.g., the fraction of cell volume that is made up of mitochondrial volume. The units of Vv are μm3 /μm3.Kidney biopsies from 15 patients were used. Five were donor biopsies performed at the time of kidney transplantation (patients 1-5, TABLE 1) and were considered normal kidney tissue. The remaining biopsies were obtained from diabetic patients with a spectrum of diabetic kidney lesions. The biopsy specimens were fixed and embedded according to routine electron microscogy protocols. Three glomeruli from each patient were selected randomly for electron microscopy. An average of 12 unbiased and systematic micrographs were obtained from each glomerulus and printed at a final magnification of x18,000.


1981 ◽  
Vol 393 (2) ◽  
pp. 145-151 ◽  
Author(s):  
P. C. M. Rosekrans ◽  
J. Lindeman ◽  
C. J. L. M. Meijer

BMJ ◽  
1967 ◽  
Vol 1 (5534) ◽  
pp. 237-238
Author(s):  
M. Taylor

2012 ◽  
Vol 208 (9) ◽  
pp. 503-509 ◽  
Author(s):  
Hiroki Miyazaki ◽  
Hiroshi Uozaki ◽  
Akihiro Tojo ◽  
Sayuri Hirashima ◽  
Sumire Inaga ◽  
...  

1992 ◽  
Vol 8 (3) ◽  
pp. 278-282 ◽  
Author(s):  
Mohammed Akhtar ◽  
Mohammed Bakry ◽  
Aziza Saad Al-Jeaid ◽  
Jeffrey A. McClintock

Author(s):  
Glenn R. Smith ◽  
Krishna Seshan ◽  
Jerome J. Wesolowski ◽  
Axel G. Berner

Determination of the mass concentration of quartz in small biopsy specimens for clinical diagnostic purposes often cannot be performed by x-ray diffraction and light microscopy. A technique utilizing transmission electron microscopy (TEM) was used to determine the quartz concentration in a human lung specimen taken from a patient suspected of having silicosis.The sample was prepared for examination by low temperature incineration in an oxygen plasma. The inorganic residue was suspended in 0.5 percent Parlodion in amyl acetate. Grids were coated with the sample suspension and subsequently shadowed with a thin film of carbon. Standard quartz particles were prepared in a similar manner.


Sign in / Sign up

Export Citation Format

Share Document