Application of low-vacuum scanning electron microscopy for renal biopsy specimens

2012 ◽  
Vol 208 (9) ◽  
pp. 503-509 ◽  
Author(s):  
Hiroki Miyazaki ◽  
Hiroshi Uozaki ◽  
Akihiro Tojo ◽  
Sayuri Hirashima ◽  
Sumire Inaga ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Biopsy ◽  
Biopsy Specimens ◽  
Scanning Electron

The preparation of glomeruli from renal biopsy specimens for scanning electron microscopy

Pathology ◽  
1982 ◽  
Vol 14 (3) ◽  
pp. 299-302 ◽  
Author(s):  
Wing-ling Ng ◽  
K.F. So ◽  
P.C. So ◽  
H.K. Ngai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Biopsy ◽  
Biopsy Specimens ◽  
Scanning Electron

Scanning Electron Microscopy of Human Jejunum in Whipple's Disease

1977 ◽  
Vol 35 ◽  
pp. 354-355
Author(s):  
John H. L. Watson ◽  
C. N. Sun
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Lamina Propria ◽  
Brush Border ◽  
Whipple’S Disease ◽  
Whipple's Disease ◽  
Biopsy Specimens ◽  
Brush Borders ◽  
Scanning Electron

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.

scholarly journals Rapid three-dimensional analysis of renal biopsy sections by low vacuum scanning electron microscopy

2011 ◽  
Vol 73 (3) ◽  
pp. 113-125 ◽  
Author(s):  
Sumire Inaga ◽  
Masako Kato ◽  
Sayuri Hirashima ◽  
Chishio Munemura ◽  
Sinichi Okada ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Biopsy ◽  
Dimensional Analysis ◽  
Three Dimensional ◽  
Scanning Electron

scholarly journals Pathological Analysis of Early Transplant Glomerulopathy in Renal Allografts Using Low-Vacuum Scanning Electron Microscopy

Nephron ◽  
2020 ◽  
pp. 1-8
Author(s):  
Hiroka Onishi ◽  
Hideyo Oguchi ◽  
Kazunobu Shinoda ◽  
Tetuo Mikami ◽  
Taichi Arai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Capillary Number ◽  
Periodic Acid ◽  
Kidney Transplant Recipients ◽  
Average Percentage ◽  
Antibody Mediated Rejection ◽  
Biopsy Specimens ◽  
Transplant Glomerulopathy ◽  
Scanning Electron

<b><i>Aim:</i></b> Low-vacuum scanning electron microscopy (LVSEM) has been reported to aid in diagnosis of renal biopsy. This study evaluated early transplant glomerulopathy in kidney transplant recipients using LVSEM. <b><i>Methods:</i></b> We selected 4 biopsies of cg0, 5 biopsies of cg1a, 5 biopsies of cg1b, and 4 biopsies of cg2 lesions that had been evaluated by light microscopy (LM) and transmission electron microscopy from recipients with acute/active or chronic, active antibody-mediated rejection (AABMR or CAABMR). Renal allograft paraffin sections (1 µm thickness) were stained with periodic acid-methenamine silver and observed using LVSEM. The cg score was based on the Banff classification. The parameter “percentage of duplicated capillary number” was calculated as follows: in 1 glomerulus with glomerular basement membrane (GBM) duplication, the total duplicated capillary number/the total number of capillaries ×100. <b><i>Results:</i></b> In all 4 biopsy specimens with AABMR showing cg0, LVSEM revealed GBM duplication not identified by LM. The average percentage of duplicated capillary number per glomerulus with GBM duplication was higher when observed by LVSEM than when observed by LM in all cg1b and cg2 biopsy specimens. <b><i>Conclusion:</i></b> LVSEM revealed early GBM duplication in AABMR. Early GBM duplication might progress in the very early phase of AABMR. GBM duplication was more frequently detected by LVSEM than by LM in biopsy specimens with early chronic, active antibody mediated rejection. Thus, LVSEM may be useful in diagnosis of early transplant glomerulopathy.

Scanning electron microscopy of biopsy specimens removed by a colonoscope from the abomasum of sheep infected with Haemonchus contortus

Parasitology ◽  
1985 ◽  
Vol 90 (2) ◽  
pp. 357-363 ◽  
Author(s):  
C. D. Nicholls ◽  
D. L. Lee ◽  
M. J. Sharpe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Parasitic Nematodes ◽  
Fibre Optic ◽  
Biopsy Specimens ◽  
Optic Endoscopy ◽  
Surrounding Areas ◽  
Scanning Electron

The abomasum of living sheep infected with 50000 larvae of Haemonchus contortus was examined before and during infection, by means of fibre optic endoscopy. Biopsy specimens were removed from the abomasum of the living sheep and were examined by means of scanning electron microscopy. Changes were noted in the surface structure of the abomasum 2 days after infection and larvae were seen on, and burrowing into, the mucosa. These changes became more pronounced as the infection proceeded, especially after day 10 of the infection when the adult worms had appeared. The behaviour of adult nematodes was observed within the abomasum and on occasions they were seen to move from surrounding areas into an area of haemorrhage, caused by removal of a biopsy specimen. Individual nematodes were removed from the abomasum by means of the biopsy forceps. Fibre optic endoscopy was shown to be a useful tool in the study of parasitic nematodes in vivo.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

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