Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag for Fluorescence Correlation Spectroscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles. Spectroscopic Behavior in Aqueous Media

2003 ◽  
Vol 68 (11) ◽  
pp. 2105-2119 ◽  
Author(s):  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof

Fluorescence behavior of octadecylrhodamine B (ORB) and the association-dependent changes in UV-VIS absorption and fluorescence spectra in aqueous solutions were studied by UV-VIS absorption spectroscopy and steady-state and time-resolved fluorometry. Spectral changes are interpreted in terms of the hydrophobically driven formation of H- and J-dimers and higher aggregates. The knowledge gained serves as a basis for the interpretation of results of the fluorescence correlation spectroscopy study of polymeric micelles labeled with ORB.

2016 ◽  
Vol 18 (25) ◽  
pp. 16766-16774 ◽  
Author(s):  
Hao Xu ◽  
Shinobu Nagasaka ◽  
Naohiro Kameta ◽  
Mitsutoshi Masuda ◽  
Takashi Ito ◽  
...  

Imaging fluorescence correlation spectroscopy is used to elucidate the rate and mechanism for diffusion of charged molecules within charged, self-assembled organic nanotubes.


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