Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag; Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles†,‡

Langmuir ◽  
2003 ◽  
Vol 19 (10) ◽  
pp. 4111-4119 ◽  
Author(s):  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof ◽  
Zdeněk Tuzar ◽  
Milena Špírková
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Water Soluble ◽  
Force Microscopy ◽  
Fluorescence Correlation ◽  
Atomic Force ◽  
Block Copolymer Micelles ◽  
Copolymer Micelles ◽  
Fluorescent Tag ◽  
Mode Atomic Force Microscopy

Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag for Fluorescence Correlation Spectroscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles. Spectroscopic Behavior in Aqueous Media

2003 ◽  
Vol 68 (11) ◽  
pp. 2105-2119 ◽  
Author(s):  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Polymeric Micelles ◽  
Aqueous Media ◽  
Correlation Spectroscopy ◽  
Water Soluble ◽  
Time Resolved ◽  
Fluorescence Correlation ◽  
Block Copolymer Micelles ◽  
Spectroscopy Studies ◽  
Fluorescent Tag

Fluorescence behavior of octadecylrhodamine B (ORB) and the association-dependent changes in UV-VIS absorption and fluorescence spectra in aqueous solutions were studied by UV-VIS absorption spectroscopy and steady-state and time-resolved fluorometry. Spectral changes are interpreted in terms of the hydrophobically driven formation of H- and J-dimers and higher aggregates. The knowledge gained serves as a basis for the interpretation of results of the fluorescence correlation spectroscopy study of polymeric micelles labeled with ORB.

Light Scattering, Atomic Force Microscopy and Fluorescence Correlation Spectroscopy Studies of Polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide) Micelles

2003 ◽  
Vol 68 (11) ◽  
pp. 2120-2138 ◽  
Author(s):  
Miroslav Štěpánek ◽  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof ◽  
Zdeněk Tuzar ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Light Scattering ◽  
Fluorescence Correlation Spectroscopy ◽  
Polymeric Nanoparticles ◽  
Correlation Spectroscopy ◽  
Force Microscopy ◽  
Fluorescence Correlation ◽  
Atomic Force ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

Polymeric nanoparticles formed by triblock copolymer polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide), PS-PVP-PEO, in aqueous media were studied by a combination of fluorescence correlation spectroscopy with other fluorescence techniques, light scattering and atomic force microscopy. The studied polymeric nanoparticles exist in the form of (i) core/shell micelles in acid solution at pH lower than 4.8 and (ii) three-layer onion micelles at higher pH. Since water is a very strong precipitant for PS, both types of micelles have kinetically frozen spherical PS cores. The cores of micelles in acid media are surrounded by soluble shells formed by partly protonated PVP and PEO, while the cores of micelles in alkaline media are surrounded by compact insoluble layers of deprotonated PVP and soluble PEO shells. The micellization behavior of PS-PVP-PEO micelles is accompanied by secondary aggregation of micelles, which is provoked by stirring, shaking and also by filtration of micellar solutions. Therefore fluorescence correlation spectroscopy (FCS), which, in contrast to light scattering techniques, does not require filtration, was used as the main experimental technique for the characterization of non-aggregated micelles. The binding of a fluorescence probe, octadecylrhodamine B (ORB), to polymeric micelles, was studied before the FCS study of micelles.

In Situ Atomic Force Microscopy Imaging of Block Copolymer Micelles Adsorbed on a Solid Substrate

Langmuir ◽  
2003 ◽  
Vol 19 (24) ◽  
pp. 10449-10453 ◽  
Author(s):  
Simon D. Connell ◽  
Stephen Collins ◽  
Johan Fundin ◽  
Zhuo Yang ◽  
Ian W. Hamley
Keyword(s):  
Atomic Force Microscopy ◽  
Block Copolymer ◽  
Solid Substrate ◽  
Microscopy Imaging ◽  
Force Microscopy ◽  
Atomic Force ◽  
Atomic Force Microscopy Imaging ◽  
Block Copolymer Micelles ◽  
Copolymer Micelles

Molecular Exchange Kinetics of Diblock Copolymer Micelles Monitored by Fluorescence Correlation Spectroscopy

2014 ◽  
Vol 3 (5) ◽  
pp. 428-432 ◽  
Author(s):  
David Schaeffel ◽  
Andreas Kreyes ◽  
Yi Zhao ◽  
Katharina Landfester ◽  
Hans-Jürgen Butt ◽  
...  
Keyword(s):  
Diblock Copolymer ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Exchange Kinetics ◽  
Copolymer Micelles ◽  
Kinetics Of
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