Hybrid Block Copolymer Micelles with Partly Hydrophobically Modified Polyelectrolyte Shells in Polar and Aqueous Media:  Experimental Study Using Fluorescence Correlation Spectroscopy, Time-Resolved Fluorescence, Light Scattering, and Atomic Force Microscopy†

2003 ◽  
Vol 107 (32) ◽  
pp. 8232-8240 ◽  
Author(s):  
Pavel Matějíček ◽  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Zdeněk Tuzar ◽  
Milena Špírková ◽  
...  
Keyword(s):  
Aqueous Media ◽  
Correlation Spectroscopy ◽  
Time Resolved ◽  
Force Microscopy ◽  
Fluorescence Correlation ◽  
Hydrophobically Modified ◽  
Atomic Force ◽  
Fluorescence Light ◽  
Block Copolymer Micelles ◽  
Hydrophobically Modified Polyelectrolyte

Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag for Fluorescence Correlation Spectroscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles. Spectroscopic Behavior in Aqueous Media

2003 ◽  
Vol 68 (11) ◽  
pp. 2105-2119 ◽  
Author(s):  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Polymeric Micelles ◽  
Aqueous Media ◽  
Correlation Spectroscopy ◽  
Water Soluble ◽  
Time Resolved ◽  
Fluorescence Correlation ◽  
Block Copolymer Micelles ◽  
Spectroscopy Studies ◽  
Fluorescent Tag

Fluorescence behavior of octadecylrhodamine B (ORB) and the association-dependent changes in UV-VIS absorption and fluorescence spectra in aqueous solutions were studied by UV-VIS absorption spectroscopy and steady-state and time-resolved fluorometry. Spectral changes are interpreted in terms of the hydrophobically driven formation of H- and J-dimers and higher aggregates. The knowledge gained serves as a basis for the interpretation of results of the fluorescence correlation spectroscopy study of polymeric micelles labeled with ORB.

Light Scattering, Atomic Force Microscopy and Fluorescence Correlation Spectroscopy Studies of Polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide) Micelles

2003 ◽  
Vol 68 (11) ◽  
pp. 2120-2138 ◽  
Author(s):  
Miroslav Štěpánek ◽  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof ◽  
Zdeněk Tuzar ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Light Scattering ◽  
Fluorescence Correlation Spectroscopy ◽  
Polymeric Nanoparticles ◽  
Correlation Spectroscopy ◽  
Force Microscopy ◽  
Fluorescence Correlation ◽  
Atomic Force ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

Polymeric nanoparticles formed by triblock copolymer polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide), PS-PVP-PEO, in aqueous media were studied by a combination of fluorescence correlation spectroscopy with other fluorescence techniques, light scattering and atomic force microscopy. The studied polymeric nanoparticles exist in the form of (i) core/shell micelles in acid solution at pH lower than 4.8 and (ii) three-layer onion micelles at higher pH. Since water is a very strong precipitant for PS, both types of micelles have kinetically frozen spherical PS cores. The cores of micelles in acid media are surrounded by soluble shells formed by partly protonated PVP and PEO, while the cores of micelles in alkaline media are surrounded by compact insoluble layers of deprotonated PVP and soluble PEO shells. The micellization behavior of PS-PVP-PEO micelles is accompanied by secondary aggregation of micelles, which is provoked by stirring, shaking and also by filtration of micellar solutions. Therefore fluorescence correlation spectroscopy (FCS), which, in contrast to light scattering techniques, does not require filtration, was used as the main experimental technique for the characterization of non-aggregated micelles. The binding of a fluorescence probe, octadecylrhodamine B (ORB), to polymeric micelles, was studied before the FCS study of micelles.

scholarly journals Review of time-resolved non-contact electrostatic force microscopy techniques with applications to ionic transport measurements

2019 ◽  
Vol 10 ◽  
pp. 617-633 ◽  
Author(s):  
Aaron Mascaro ◽  
Yoichi Miyahara ◽  
Tyler Enright ◽  
Omur E Dagdeviren ◽  
Peter Grütter
Keyword(s):  
Atomic Force Microscopy ◽  
Electrostatic Force ◽  
Oscillation Period ◽  
Electrostatic Force Microscopy ◽  
Time Varying ◽  
Time Resolved ◽  
Battery Materials ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Techniques

Recently, there have been a number of variations of electrostatic force microscopy (EFM) that allow for the measurement of time-varying forces arising from phenomena such as ion transport in battery materials or charge separation in photovoltaic systems. These forces reveal information about dynamic processes happening over nanometer length scales due to the nanometer-sized probe tips used in atomic force microscopy. Here, we review in detail several time-resolved EFM techniques based on non-contact atomic force microscopy, elaborating on their specific limitations and challenges. We also introduce a new experimental technique that can resolve time-varying signals well below the oscillation period of the cantilever and compare and contrast it with those previously established.

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