Effect of lipophilic character of inhibitor on inhibition of rat serum N-acetyl-L-tyrosine ethylesterase

1970 ◽  
Vol 35 (9) ◽  
pp. 2650-2655 ◽  
Author(s):  
E. Simonianová ◽  
M. Petáková ◽  
M. Rybák
Keyword(s):  
Rat Serum ◽  
Lipophilic Character

FUNCTIONAL HETEROGENEITY OF THE THYROID AND POSSIBLE PRESENCE OF IODOTYROSINE-LIKE COMPOUNDS IN NORMAL SERUM

1965 ◽  
Vol 48 (2) ◽  
pp. 199-208 ◽  
Author(s):  
J. D. Wiener
Keyword(s):  
Human Subjects ◽  
Thyroid Tissue ◽  
Normal Serum ◽  
Schematic Model ◽  
Functional Heterogeneity ◽  
Rat Serum ◽  
Iodine Metabolism ◽  
Normal Rat ◽  
Isotope Equilibrium ◽  
Equilibrium Experiment

ABSTRACT After the administration of 131I to normal animals or human subjects, labelled thyroxine and triiodothyronine, but at most traces of labelled iodotyrosines can be detected in the serum. However, several investigators using various methods claim to have found considerable amounts of one or both of these iodotyrosines when assaying the stable (non-radioactive) iodinated compounds in the serum. Considering the available evidence as convincing for the present, an attempt has been made to explain this discrepancy. A schematic model of the thyroidal iodine metabolism is proposed, based on (a) the hypothesis that the iodotyrosines are present in the circulation in a »masked« form (i. e. protected against deiodination), and (b) the known functional heterogeneity of the thyroid tissue. This heterogeneity should be of a qualitative as well as quantitative nature. As the physical decay rate of 131I is short in comparison with the turnover rate of the masked iodotyrosine pool, an isotope equilibrium experiment with rats was carried out, using the long-lived isotope 125I. The results of this experiment, viewed together with those of a similar investigation published by others, seem to lend support to the proposed mechanism. The presence of non-negligible amounts of a diiodotyrosine-like compound in normal rat serum seems fairly well established.

TSH DETERMINATION IN RAT SERUM BY A HOMOLOGOUS RADIOIMMUNOASSAY

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S127
Author(s):  
J. Golstein ◽  
L. Vanhaelst ◽  
M. Bonnyns ◽  
G. Rothenbuchner
Keyword(s):  
Rat Serum

Regulation of insulin-like growth factor-binding protein-1 in rat serum

Diabetes ◽  
1994 ◽  
Vol 43 (2) ◽  
pp. 232-239 ◽  
Author(s):  
M. S. Lewitt ◽  
H. Saunders ◽  
J. L. Phyual ◽  
R. C. Baxter
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Rat Serum ◽  
Factor Binding

Rat serum hydrolases cleaving N-acetyl-L-tyrosine ethyl ester

1974 ◽  
Vol 39 (7) ◽  
pp. 1925-1932
Author(s):  
E. Simonianová ◽  
M. Petáková
Keyword(s):  
Ethyl Ester ◽  
Rat Serum

Total lactate dehydrogenase and its isoenzymes in serum in the presence of penicillamine and other sulfhydryl compounds.

1977 ◽  
Vol 23 (2) ◽  
pp. 229-233 ◽  
Author(s):  
L L Gershbein ◽  
K G Raikoff
Keyword(s):  
Lactate Dehydrogenase ◽  
Disc Electrophoresis ◽  
The Other ◽  
Rat Serum ◽  
Initial Concentration ◽  
Ldh Isoenzymes ◽  
Allosteric Effectors ◽  
Sulfhydryl Compounds

Abstract Toward delineation of changes in total lactate dehydrogenase (LDH) and in the distribution of LDH isoenzymes as assessed by polyacrylamide disc electrophoresis, we inbucated human and rat sera with various agents, notably sulfhydryl compounds. Although artefacts were apparent when these agents were used without preliminary adjustment of pH, we saw little alteration in total unitage when one or two volumes of serum was mixed with one volume of any of several thiols, especially penicillamine, at an initial concentration of 0.4 mol/liter and pH 7.0-7.5. Under these conditions, penicillamine caused a loss in LDH-5 after incubation for 1 h at 25 degrees C together with small decreases in mobility of the other four isoenzymes toward the anode. A zymosan region appeared below the albumin and tracking dye area. With longer periods of incubation of rat serum with penicillamine or alpha-mercaptosuccinate, a novel band in the zymogram was noted just above the LDH-4 peak. The observations are discussed in terms of allosteric effectors.

scholarly journals Is rosuvastatin protective against on noise-induced oxidative stress in rat serum?

2015 ◽  
Vol 17 (74) ◽  
pp. 11 ◽  
Author(s):  
EmineRabia Koc ◽  
Semsettin Sahın ◽  
Alevtina Ersoy ◽  
Atilla Ilhan ◽  
HaydarAli Erken
Keyword(s):  
Oxidative Stress ◽  
Rat Serum
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