scholarly journals Limited Evidence for Infection of Urban and Peri-urban Nonhuman Primates with Zika and Chikungunya Viruses in Brazil

mSphere ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Andres Moreira-Soto ◽  
Ianei de Oliveira Carneiro ◽  
Carlo Fischer ◽  
Marie Feldmann ◽  
Beate M. Kümmerer ◽  
...  

Since 2013, Zika virus (ZIKV) and chikungunya virus (CHIKV) have infected millions of people in the Americas via urban transmission cycles. Nonhuman primates (NHP) are involved in sylvatic transmission cycles maintaining ZIKV and CHIKV in the Old World. We tested NHP sampled during 2012 to 2017 in urban and peri-urban areas severely affected by ZIKV and CHIKV in Brazil. Seroprevalence and antibody titers were low for both viruses. Additionally, we found evidence for infection by heterologous viruses eliciting cross-reactive antibodies. Our data suggest that urban or peri-urban NHP are not easily infected by ZIKV and CHIKV despite intense local transmission. These data may imply that the ZIKV and CHIKV outbreaks in the Americas cannot be sustained in urban or peri-urban NHP once human population immunity limits urban transmission cycles. Investigation of diverse animals is urgently required to determine the fate of the ZIKV and CHIKV outbreaks in the Americas.

2016 ◽  
Vol 9 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Ivana Hrnjaković Cvjetković ◽  
Tamaš Petrović ◽  
Dušan Petrić ◽  
Dejan Cvjetković ◽  
Gordana Kovačević ◽  
...  

Chikungunya virus is an Arbo virus belonging to the family Togaviridae. In urban areas, antropophilic Aedes aegypti and Aedes albopictus mosquitoes are vectors for virus transmission to human population. Chikungunya virus has attracted the professional and scientifi c public attention in 2013 causing a massive outbreak on the American continent. In Europe, autochthonous transmissions of Chikungunya virus infections have beenrecorded in Italy in 2007 as well as in France in 2010 and 2014. Usutu virus is a RNA virus from the family Flaviviridae. Th e virus circulates in a transmission cycle between wild birds and Culex mosquitoes. The virus has been detected in numerous bird species across Europe. Manifestations recorded in humans include meningoencephalitis and skin rash. First human cases in Europe were recorded in immunocompromised individuals in Italy in 2009. Spirochete Borrelia burgdorferi sensu lato is transmitted to humans by ticks and causes Lyme disease, a multisystemic disease with dermatological, neurological, cardiological or articular manifestations. Ninety three persons interviewed about risk factors for vector-borne infections were examined. The examination was performed using commercial ELISA IgG for Chikungunya and Usutu virus and ELISA IgM and IgG test for Borrelia burgdorferi in line with manufacturer’s instructions (Euroimmun, Germany). Out of 93 examined individuals Usutu virus specific IgG antibodies were identified in 7.5% (7/93) persons. Th e results of ELISA IgG test for Chikungunya virus were negative in the majority of tested samples, whereas 7.5% (7/93) of samples revealed borderline result. In 9.7% (9/93) participants, antibodies against Borrelia burgdorferi were detected only by ELISA IgM test. Recent infection with Borrelia burgdorferi was confi rmed in 2.15% (2/93) individuals, whereas IgG antibodies against Borrelia burgdorferi were detected in only one participant. Th e obtained results indicated that Usutu virus is active in the territory of South Bačka District contrary to Chikungunya virus as well as that Borrelia burgdorferi is an important pathogen in the investigated region.


2016 ◽  
Author(s):  
Henri Jupille ◽  
Goncalo Seixas ◽  
Laurence Mousson ◽  
Carla A. Sousa ◽  
Anna-Bella Failloux

AbstractBackground:Since its emergence in 2007 in Micronesia and Polynesia, the arthropod-borne flavivirus Zika virus (ZIKV) has spread in the Americas and the Caribbean, following first detection in Brazil in May 2015. The risk of ZIKV emergence in Europe increases as imported cases are repeatedly reported. Together with chikungunya virus (CHIKV) and dengue virus (DENV), ZIKV is transmitted by Aedes mosquitoes. Any countries where these mosquitoes are present could be potential sites for future ZIKV outbreak.Methodology/Principal Findings:Mosquito females were challenged with an Asian genotype of ZIKV. Fully engorged mosquitoes were then maintained in insectary conditions (28°±1°C, 16h:8h light:dark cycle and 80% humidity). 16-24 mosquitoes from each population were examined at 3, 6, 9 and 14 days postinfection to estimate the infection, disseminated infection and transmission rates. Based on these experimental infections, we demonstrated that Ae. albopictus from France were not very susceptible to ZIKV.Conclusions/Significance:In combination with the restricted distribution and lower population densities of European Ae. albopictus, our results corroborate the low risk for ZIKV to expand into most parts of Europe with the possible exception of the warmest regions bordering the Mediterranean coastline.Author summaryIn May 2015, local transmission of Zika virus (ZIKV) was reported in Brazil and since then, more than 1.5 million human cases have been reported in Latin America and the Caribbean. This arbovirus, primarily found in Africa and Asia, is mainly transmitted by Aedes mosquitoes, Aedes aegypti and Aedes albopictus. Viremic travelers returning from America to European countries where Ae. albopictus is established can become the source for local transmission of ZIKV. In order to estimate the risk of seeding ZIKV into local mosquito populations, the ability of European Ae. aegypti and Ae. albopictus to transmit ZIKV was measured using experimental infections. We demonstrated that Ae. albopictus and Ae. aegypti from Europe were not very susceptible to ZIKV. The threat for a Zika outbreak in Europe should be limited.


Author(s):  
Herman Herman ◽  
Demi Adidrana ◽  
Nico Surantha ◽  
Suharjito Suharjito

The human population significantly increases in crowded urban areas. It causes a reduction of available farming land. Therefore, a landless planting method is needed to supply the food for society. Hydroponics is one of the solutions for gardening methods without using soil. It uses nutrient-enriched mineral water as a nutrition solution for plant growth. Traditionally, hydroponic farming is conducted manually by monitoring the nutrition such as acidity or basicity (pH), the value of Total Dissolved Solids (TDS), Electrical Conductivity (EC), and nutrient temperature. In this research, the researchers propose a system that measures pH, TDS, and nutrient temperature values in the Nutrient Film Technique (NFT) technique using a couple of sensors. The researchers use lettuce as an object of experiment and apply the k-Nearest Neighbor (k-NN) algorithm to predict the classification of nutrient conditions. The result of prediction is used to provide a command to the microcontroller to turn on or off the nutrition controller actuators simultaneously at a time. The experiment result shows that the proposed k-NN algorithm achieves 93.3% accuracy when it is k = 5.


Author(s):  
Rebecca A Zimler ◽  
Donald A Yee ◽  
Barry W Alto

Abstract Recurrence of local transmission of Zika virus in Puerto Rico is a major public health risk to the United States, where mosquitoes Aedes aegypti (Linnaeus) and Aedes mediovittatus (Coquillett) are abundant. To determine the extent to which Ae. mediovittatus are capable of transmitting Zika virus and the influence of viremia, we evaluated infection and transmission in Ae. mediovittatus and Ae. aegypti from Puerto Rico using serial dilutions of infectious blood. Higher doses of infectious blood resulted in greater infection rates in both mosquitoes. Aedes aegypti females were up to twice as susceptible to infection than Ae. mediovittatus, indicating a more effective midgut infection barrier in the latter mosquito species. Aedes aegypti exhibited higher disseminated infection (40–95%) than Ae. mediovittatus (<5%), suggesting a substantial midgut escape barrier in Ae. mediovittatus. For Ae. aegypti, transmission rates were low over a range of doses of Zika virus ingested, suggesting substantial salivary gland barriers.


2019 ◽  
Vol 113 (11) ◽  
pp. 678-684 ◽  
Author(s):  
I-Ching Sam ◽  
Magelda Montoya ◽  
Chong Long Chua ◽  
Yoke Fun Chan ◽  
Andrew Pastor ◽  
...  

Abstract Background Zika virus (ZIKV) is believed to be endemic in Southeast Asia. However, there have been few Zika cases reported to date in Malaysia, which could be due to high pre-existing levels of population immunity. Methods To determine Zika virus (ZIKV) seroprevalence in Kuala Lumpur, Malaysia, 1085 serum samples from 2012, 2014–2015 and 2017 were screened for anti-ZIKV antibodies using a ZIKV NS1 blockade-of-binding assay. Reactive samples were confirmed using neutralization assays against ZIKV and the four dengue virus (DENV) serotypes. A sample was possible ZIKV seropositive with a ZIKV 50% neutralization (NT50) titre ≥20. A sample was probable ZIKV seropositive if, in addition, all DENV NT50 titres were <20 or the ZIKV NT50 titre was >4-fold greater than the highest DENV NT50 titre. Results We found low rates of possible ZIKV seropositivity (3.3% [95% confidence interval {CI} 2.4 to 4.6]) and probable ZIKV seropositivity (0.6% [95% CI 0.3 to 1.4]). Possible ZIKV seropositivity was independently associated with increasing age (odds ratio [OR] 1.04 [95% CI 1.02 to 1.06], p<0.0001) and male gender (OR 3.5 [95% CI 1.5 to 8.6], p=0.005). Conclusions The low ZIKV seroprevalence rate, a proxy for population immunity, does not explain the low incidence of Zika in dengue-hyperendemic Kuala Lumpur. Other factors, such as the possible protective effects of pre-existing flavivirus antibodies or reduced transmission by local mosquito vectors, should be explored. Kuala Lumpur is at high risk of a large-scale Zika epidemic.


Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1283
Author(s):  
Katendi Changula ◽  
Edgar Simulundu ◽  
Boniface Pongombo Lombe ◽  
Eri Nakayama ◽  
Hiroko Miyamoto ◽  
...  

Ebolaviruses and marburgviruses are filoviruses that are known to cause severe hemorrhagic fever in humans and nonhuman primates (NHPs). While some bat species are suspected to be natural reservoirs of these filoviruses, wild NHPs often act as intermediate hosts for viral transmission to humans. Using an enzyme-linked immunosorbent assay, we screened two NHP species, wild baboons and vervet monkeys captured in Zambia, for their serum IgG antibodies specific to the envelope glycoproteins of filoviruses. From 243 samples tested, 39 NHPs (16%) were found to be seropositive either for ebolaviruses or marburgviruses with endpoint antibody titers ranging from 100 to 25,600. Interestingly, antibodies reactive to Reston virus, which is found only in Asia, were detected in both NHP species. There was a significant difference in the seropositivity for the marburgvirus antigen between the two NHP species, with baboons having a higher positive rate. These results suggest that wild NHPs in Zambia might be nonlethally exposed to these filoviruses, and this emphasizes the need for continuous monitoring of filovirus infection in wild animals to better understand the ecology of filoviruses and to assess potential risks of outbreaks in humans in previously nonendemic countries.


Author(s):  
Albert Utama ◽  
Sutarki Sutisna

The Living Bot is a project where future residential buildings will adapt to the times. In the coming year, the human population will continue to grow, so that it will use the land as a place for various needs such as shelter, activities, and other things. Along with this increase in human population, the land will also shrink while the land itself is needed so that humans can meet their food needs either from farming (plants), or through livestock (animal). Therefore, The Living Bot created a system in which human implementation begins to adapt to the life in which they live by implementing a residential system that can produce their own food with plantings that maximize vertical land. This form of shelter can be used as a system so that its application can be carried out. Adaptations that are carried out are by changing the lifestyle of humans to the use of technology. The lifestyle that must adapt is by farming, assisted by A.I. technology. because humans in urban areas do not have a background in growing a food crop. Therefore technology is present in helping urban communities, also assisted by modern planting methods such as using hydroponics, aquaponics, aeroponics, and indoor planting techniques assisted by artificial light such as LEDs. Keywords: Adaptation; Techonology Abstrak The Living Bot merupakan sebuah proyek dimana bangunan hunian pada masa depan akan beradaptasi dengan perkembangan zaman. Pada tahun yang akan datang, populasi manusia akan terus bertambah, sehingga akan menggunakan lahan sebagai tempat untuk berbagai macam kebutuhan seperti tempat tinggal, aktivitas, dan hal lainnya. Seiring dengan pertambahan populasi manusia ini, lahan juga akan semakin menyempit sedangkan lahan sendiri diperlukan agar manusia dapat memenuhi kebutuhan pangannya baik dari hasil bertani (tumbuh-tumbuhan), ataupun melalui peternakan (hewani). Maka dari itu The Living Bot membuat suatu sistem yaitu dimana implementasi manusia mulai beradaptasi dengan kehidupan tempat tinggalnya dengan menerapkan sistem hunian yang dapat menghasilkan makanannya sendiri dengan penanaman-penanaman yang memaksimalkan lahan secara vertikal.Bentuk hunian seperti ini dapat dijadikan sebuah sistem sehingga penerapannya dapat dilakukan di berbagai hunian Adaptasi yang dilakukan adalah dengan mengubah gaya hidup manusia sampai kepada pengunaan teknologi. Adapun gaya hidup yang harus beradaptasi adalah dengan bercocok tanam, dengan dibantu oleh teknologi A.I. karena manusia yang ada di perkotaan tidak memiliki latar belakang dalam menanam sebuah tanaman pangan. Maka dari itu teknologi hadir dalam membantu masyarakat kota, juga dibantu oleh metode menanam yang modern seperti menggunakan hidroponik, akuaponik, aeroponik, dan teknik penanaman indoor yang dibantu oleh cahaya buatan seperti LED.


Insects ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 177 ◽  
Author(s):  
Tereza Magalhaes ◽  
Alexis Robison ◽  
Michael Young ◽  
William Black ◽  
Brian Foy ◽  
...  

In urban settings, chikungunya, Zika, and dengue viruses are transmitted by Aedes aegypti mosquitoes. Since these viruses co-circulate in several regions, coinfection in humans and vectors may occur, and human coinfections have been frequently reported. Yet, little is known about the molecular aspects of virus interactions within hosts and how they contribute to arbovirus transmission dynamics. We have previously shown that Aedes aegypti exposed to chikungunya and Zika viruses in the same blood meal can become coinfected and transmit both viruses simultaneously. However, mosquitoes may also become coinfected by multiple, sequential feeds on single infected hosts. Therefore, we tested whether sequential infection with chikungunya and Zika viruses impacts mosquito vector competence. We exposed Ae. aegypti mosquitoes first to one virus and 7 days later to the other virus and compared infection, dissemination, and transmission rates between sequentially and single infected groups. We found that coinfection rates were high after sequential exposure and that mosquitoes were able to co-transmit both viruses. Surprisingly, chikungunya virus coinfection enhanced Zika virus transmission 7 days after the second blood meal. Our data demonstrate heterologous arbovirus synergism within mosquitoes, by unknown mechanisms, leading to enhancement of transmission under certain conditions.


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