scholarly journals Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.

1988 ◽  
Vol 8 (5) ◽  
pp. 1985-1992 ◽  
Author(s):  
R L Feinbaum ◽  
F M Ausubel
Keyword(s):  
Arabidopsis Thaliana ◽  
High Intensity ◽  
Chalcone Synthase ◽  
State Level ◽  
Fold Increase ◽  
Single Copy ◽  
Light Treatment ◽  
Cross Hybridization ◽  
Chalcone Synthase Gene ◽  
High Intensity Light

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.

Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene

1988 ◽  
Vol 8 (5) ◽  
pp. 1985-1992
Author(s):  
R L Feinbaum ◽  
F M Ausubel
Keyword(s):  
Arabidopsis Thaliana ◽  
High Intensity ◽  
Chalcone Synthase ◽  
State Level ◽  
Fold Increase ◽  
Single Copy ◽  
Light Treatment ◽  
Cross Hybridization ◽  
Chalcone Synthase Gene ◽  
High Intensity Light

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.

scholarly journals Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots

2019 ◽  
Vol 20 (20) ◽  
pp. 5131
Author(s):  
Barczak-Brzyżek Anna ◽  
Brzyżek Grzegorz ◽  
Koter Marek ◽  
Gawroński Piotr ◽  
Filipecki Marcin
Keyword(s):  
Arabidopsis Thaliana ◽  
Stress Responses ◽  
High Intensity ◽  
Light Stress ◽  
Adaptive Responses ◽  
Stress Signal ◽  
Below Ground ◽  
Stress Signals ◽  
Transcriptomic Changes ◽  
High Intensity Light

In full sunlight, plants often experience a light intensity exceeding their photosynthetic capacity and causing the activation of a set of photoprotective mechanisms. Numerous reports have explained, on the molecular level, how plants cope with light stress locally in photosynthesizing leaves; however, the response of below-ground organs to above-ground perceived light stress is still largely unknown. Since small RNAs are potent integrators of multiple processes including stress responses, here, we focus on changes in the expression of root miRNAs upon high-intensity-light (HL) stress. To achieve this, we used Arabidopsis thaliana plants growing in hydroponic conditions. The expression of several genes that are known as markers of redox changes was examined over time, with the results showing that typical HL stress signals spread to the below-ground organs. Additionally, micro-transcriptomic analysis of systemically stressed roots revealed a relatively limited reaction, with only 17 up-regulated and five down-regulated miRNAs. The differential expression of candidates was confirmed by RT-qPCR. Interestingly, the detected differences in miRNA abundance disappeared when the roots were separated from the shoots before HL treatment. Thus, our results show that the light stress signal is induced in rosettes and travels through the plant to affect root miRNA levels. Although the mechanism of this regulation is unknown, the engagement of miRNA may create a regulatory platform orchestrating adaptive responses to various simultaneous stresses. Consequently, further research on systemically HL-regulated miRNAs and their respective targets has the potential to identify attractive sequences for engineering stress tolerance in plants.

PIGMENT COMPOSITION OF THE GREEN ALGA HAEMATOCOCCUS PLUVIALIS DURING ACTION OF MULTIPLE ASTAXANTHIN ACCUMULATION INDUCTORS

2020 ◽  
pp. 386-388
Author(s):  
R. Goncharik ◽  
E. Kulikov ◽  
A. Selishcheva
Keyword(s):  
Green Alga ◽  
Photosynthetic Pigments ◽  
High Intensity ◽  
Culture Medium ◽  
Haematococcus Pluvialis ◽  
Light Treatment ◽  
Pigment Composition ◽  
Prolonged Action ◽  
High Intensity Light ◽  
In Cells

The content of astaxanthin, including its mono- and diesters, and photosynthetic pigments, was studied in cells of H. pluvialis strain IBCE-H17 under combined prolonged action of several inducers of astaxanthin accumulation. The ineffectiveness of the simultaneous introduction of several inducers of astaxanthin accumulation in the culture medium during high-intensity light treatment was shown.

Metabolite profiling of Arabidopsis thaliana (L.) plants transformed with an antisense chalcone synthase gene

Metabolomics ◽  
2005 ◽  
Vol 1 (2) ◽  
pp. 181-198 ◽  
Author(s):  
Gwénaëlle Le Gall ◽  
Stine B. Metzdorff ◽  
Jan Pedersen ◽  
Richard N. Bennett ◽  
Ian J. Colquhoun
Keyword(s):  
Arabidopsis Thaliana ◽  
Metabolite Profiling ◽  
Chalcone Synthase ◽  
Chalcone Synthase Gene

Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element

1988 ◽  
Vol 212 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Anette Hermann ◽  
Wolfgang Schulz ◽  
Klaus Hahlbrock
Keyword(s):  
Chalcone Synthase ◽  
Single Copy ◽  
Chalcone Synthase Gene

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

Molecular Evolution of Chalcone Synthase Gene Superfamily in Plants

2015 ◽  
Vol 50 (1) ◽  
pp. 55
Author(s):  
Bao Ying ◽  
Guo Changfeng ◽  
Chen Shaohua ◽  
Liu Mei
Keyword(s):  
Molecular Evolution ◽  
Chalcone Synthase ◽  
Chalcone Synthase Gene
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