Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element

1988 ◽  
Vol 212 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Anette Hermann ◽  
Wolfgang Schulz ◽  
Klaus Hahlbrock
1988 ◽  
Vol 8 (5) ◽  
pp. 1985-1992 ◽  
Author(s):  
R L Feinbaum ◽  
F M Ausubel

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.


1988 ◽  
Vol 8 (5) ◽  
pp. 1985-1992
Author(s):  
R L Feinbaum ◽  
F M Ausubel

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.


2015 ◽  
Vol 50 (1) ◽  
pp. 55
Author(s):  
Bao Ying ◽  
Guo Changfeng ◽  
Chen Shaohua ◽  
Liu Mei

2001 ◽  
Vol 151 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Gareth I. Jenkins ◽  
Joanne C. Long ◽  
Helena K. Wade ◽  
Matthew R. Shenton ◽  
Tatiana N. Bibikova

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