scholarly journals FLP Recombinase-Mediated Induction of Cu/Zn-Superoxide Dismutase Transgene Expression Can Extend the Life Span of Adult Drosophila melanogaster Flies

1999 ◽  
Vol 19 (1) ◽  
pp. 216-228 ◽  
Author(s):  
Jingtao Sun ◽  
John Tower
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Life Span ◽  
Transgene Expression ◽  
Experimental Manipulation ◽  
Heat Pulse ◽  
Limiting Factor ◽  
Negative Effects ◽  
Flp Recombinase ◽  
The Mean

ABSTRACT Yeast FLP recombinase was used in a binary transgenic system (“FLP-OUT”) to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target construct in which expression of catalase or Cu/ZnSOD cDNAs was driven by the constitutive actin5C promoter. In this way a brief heat pulse (120 or 180 min, total) of young adult flies activated transgene expression for the rest of the life span. FLP-OUT allows the effects of induced transgene expression to be analyzed in control (no heat pulse) and experimental (heat pulse) populations with identical genetic backgrounds. Under the conditions used, the heat pulse itself always had neutral or slightly negative effects on the life span. Catalase overexpression significantly increased resistance to hydrogen peroxide but had neutral or slightly negative effects on the mean life span. Cu/ZnSOD overexpression extended the mean life span up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD had no added benefit, presumably due to a preexisting excess of catalase. The data suggest that oxidative damage is one rate-limiting factor for the life span of adult Drosophila. Finally, experimental manipulation of the genetic background demonstrated that the life span is affected by epistatic interactions between the transgene and allele(s) at other loci.

Induced Overexpression of Mitochondrial Mn-Superoxide Dismutase Extends the Life Span of Adult Drosophila melanogaster

Genetics ◽  
2002 ◽  
Vol 161 (2) ◽  
pp. 661-672
Author(s):  
Jingtao Sun ◽  
Donna Folk ◽  
Timothy J Bradley ◽  
John Tower
Keyword(s):  
Drosophila Melanogaster ◽  
Life Span ◽  
Adult Life ◽  
Heat Pulse ◽  
Maximum Life Span ◽  
Adult Life Span ◽  
Flp Recombinase ◽  
Transgenic Lines ◽  
Added Benefit ◽  
Adult Drosophila

Abstract A transgenic system (“FLP-out”) based on yeast FLP recombinase allowed induced overexpression of MnSOD enzyme in adult Drosophila melanogaster. With FLP-out a brief heat pulse (HP) of young, adult flies triggered the rearrangement and subsequent expression of a MnSOD transgene throughout the adult life span. Control (no HP) and overexpressing (HP) flies had identical genetic backgrounds. The amount of MnSOD enzyme overexpression achieved varied among six independent transgenic lines, with increases up to 75%. Life span was increased in proportion to the increase in enzyme. Mean life span was increased by an average of 16%, with some lines showing 30-33% increases. Maximum life span was increased by an average of 15%, with one line showing as much as 37% increase. Simultaneous overexpression of catalase with MnSOD had no added benefit, consistent with previous observations that catalase is present in excess in the adult fly with regard to life span. Cu/ZnSOD overexpression also increases mean and maximum life span. For both MnSOD and Cu/ZnSOD lines, increased life span was not associated with decreased metabolic activity, as measured by O2 consumption.

Phenotypic consequences of copper-zinc superoxide dismutase overexpression in Drosophila melanogaster

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 867-872 ◽  
Author(s):  
Brian E. Staveley ◽  
John P. Phillips ◽  
Arthur J. Hilliker
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Ionizing Radiation ◽  
Life Span ◽  
Adult Life ◽  
Small Region ◽  
Laboratory Culture ◽  
Adult Life Span ◽  
Copper Zinc Superoxide Dismutase ◽  
Diploid Level

We report here the isolation of a tandem duplication of a small region of the third chomosome of Drosophila melanogaster containing the Cu–Zn superoxide dismutase (cSOD) gene. This duplication is associated with a dosage-dependent increase in cSOD activity. The biological consequences of hypermorphic levels of cSOD in genotypes carrying this duplication have been investigated under diverse conditions of oxygen stress imposed by acute exposure to ionizing radiation, chronic exposure to paraquat, and the normoxia of standard laboratory culture. We find that a 50% increase in cSOD activity above the normal diploid level confers increased resistance to ionizing radiation and, in contrast, confers decreased resistance to the superoxide-generating agent paraquat. The duplication is associated with a minor increase in adult life-span under conditions of normoxia. These results reveal important features of the biological function of cSOD within the context of the overall oxygen defense system of Drosophila.Key words: gene duplication, paraquat, ionizing radiation, life-span.

scholarly journals Phenotypic rescue by a bovine transgene in a Cu/Zn superoxide dismutase-null mutant of Drosophila melanogaster

1994 ◽  
Vol 14 (2) ◽  
pp. 1302-1307
Author(s):  
I Reveillaud ◽  
J Phillips ◽  
B Duyf ◽  
A Hilliker ◽  
A Kongpachith ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Life Span ◽  
Male Fertility ◽  
Transcriptional Control ◽  
Adult Life ◽  
P Element ◽  
Normal Male ◽  
Null Mutant ◽  
Adult Life Span

Null mutants for Cu/Zn superoxide dismutase (CuZnSOD) in Drosophila melanogaster are male sterile, have a greatly reduced adult life span, and are hypersensitive to paraquat. We have introduced a synthetic bovine CuZnSOD transgene under the transcriptional control of the D. melanogaster 5C actin promoter into a CuZnSOD-null mutant of D. melanogaster. This was carried out by P-element-mediated transformation of the Drosophila-bovine CuZnSOD transgene into a CuZnSOD+ recipient strain followed by genetic crossing of the transgene into a strain carrying the CuZnSOD-null mutation, cSODn108. The resulting transformants express bovine CuZnSOD exclusively to about 30% of normal Drosophila CuZnSOD levels. Expression of the Drosophila-bovine CuZnSOD transgene in the CuZnSOD-null mutant rescues male fertility and resistance to paraquat to apparently normal levels. However, adult life span is restored to only 30% of normal, and resistance to hyperoxia is 90% of that found in control flies. This striking differential restoration of pleiotropic phenotypes could be the result of a threshhold of CuZnSOD expression necessary for normal male fertility and resistance to the toxicity of paraquat or hyperoxia which is lower than the threshold required to sustain a normal adult life span. Alternatively, the differential rescue of fertility, resistance to active oxygen, and life span might indicate different cell-specific transcriptional requirements for these functions which are normally provided by the control elements of the native CuZnSOD gene but are only partly compensated for by the transcriptional control elements of the actin 5C promoter.

scholarly journals Oxidative stress and ageing in Caenorhabditis elegans

1993 ◽  
Vol 292 (2) ◽  
pp. 605-608 ◽  
Author(s):  
J R Vanfleteren
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Caenorhabditis Elegans ◽  
Life Span ◽  
Superoxide Anion ◽  
Superoxide Dismutase Activity ◽  
Activity Levels ◽  
Superoxide Anion Production ◽  
Specific Increase ◽  
The Mean

Mutations in the age-1 gene double both the mean and maximum life span of Caenorhabditis elegans. They also result in an age-specific increase of catalase and Cu/Zn superoxide dismutase activity levels. The higher superoxide dismutase activity levels in age-1 mutants confer hyperresistance to the superoxide-anion-generating drug paraquat. The rate of superoxide anion production by microsome fractions declines linearly with age in age-1(+) worms, but, after an initial decline, is stabilized at a higher level in senescent age-1 mutant nematodes. These results clearly show that oxidative stress resistance and potential life span are correlated in this organism, and they suggest that the natural product of age-1 either directly or indirectly downregulates the activities of several other genes as a function of age.

scholarly journals Life extension in Drosophila melanogasteras a result of development in conditions of high larval density

2018 ◽  
Vol 15 (2) ◽  
pp. 167-173
Author(s):  
A. S. Karaman ◽  
A. M. Vaiserman ◽  
A. K. Koliada ◽  
O. G. Zabuga ◽  
A. V. Pisaruk ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Life Expectancy ◽  
Life Span ◽  
Larval Density ◽  
Reproductive Activity ◽  
Life Extension ◽  
Maximum Life Span ◽  
Males And Females ◽  
The Mean ◽  
Density Methods

Aim. To investigate the life expectancy and reproductive activity of Drosophila melanogaster that developed in conditions of increased larval density. Methods. Mean and maximum life span were determined in males and females in the different experimental groups. The reproductive activity was evaluated by counting the total number of eggs laid by one female per day. Results. A significant increase of the mean life span compared to control was observed in adults that hatched from pupae during the first and second days after the beginning of the emergence: males — 24 % and 23.5 %, females — 23.8 % and 29.3 % respectively. The level of reproductive activity (fecundity) is statistically lower in two groups which hatched last. Conclusions. Based on the results obtained, we suggest that development in conditions of increased larval density can lead to increase in the life span of D. melanogaster.Keywords: life span, Drosophila melanogaster, reproductive activity, development.

scholarly journals Phenotypic rescue by a bovine transgene in a Cu/Zn superoxide dismutase-null mutant of Drosophila melanogaster.

1994 ◽  
Vol 14 (2) ◽  
pp. 1302-1307 ◽  
Author(s):  
I Reveillaud ◽  
J Phillips ◽  
B Duyf ◽  
A Hilliker ◽  
A Kongpachith ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Life Span ◽  
Male Fertility ◽  
Transcriptional Control ◽  
Adult Life ◽  
P Element ◽  
Normal Male ◽  
Null Mutant ◽  
Adult Life Span

Null mutants for Cu/Zn superoxide dismutase (CuZnSOD) in Drosophila melanogaster are male sterile, have a greatly reduced adult life span, and are hypersensitive to paraquat. We have introduced a synthetic bovine CuZnSOD transgene under the transcriptional control of the D. melanogaster 5C actin promoter into a CuZnSOD-null mutant of D. melanogaster. This was carried out by P-element-mediated transformation of the Drosophila-bovine CuZnSOD transgene into a CuZnSOD+ recipient strain followed by genetic crossing of the transgene into a strain carrying the CuZnSOD-null mutation, cSODn108. The resulting transformants express bovine CuZnSOD exclusively to about 30% of normal Drosophila CuZnSOD levels. Expression of the Drosophila-bovine CuZnSOD transgene in the CuZnSOD-null mutant rescues male fertility and resistance to paraquat to apparently normal levels. However, adult life span is restored to only 30% of normal, and resistance to hyperoxia is 90% of that found in control flies. This striking differential restoration of pleiotropic phenotypes could be the result of a threshhold of CuZnSOD expression necessary for normal male fertility and resistance to the toxicity of paraquat or hyperoxia which is lower than the threshold required to sustain a normal adult life span. Alternatively, the differential rescue of fertility, resistance to active oxygen, and life span might indicate different cell-specific transcriptional requirements for these functions which are normally provided by the control elements of the native CuZnSOD gene but are only partly compensated for by the transcriptional control elements of the actin 5C promoter.

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