Effects of Cu-Zn Superoxide Dismutase Overexpression on Life Span and Resistance to Oxidative Stress in Transgenic Drosophila melanogaster

1993 ◽  
Vol 301 (1) ◽  
pp. 34-40 ◽  
Author(s):  
W.C. Orr ◽  
R.S. Sohal
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Life Span ◽  
Transgenic Drosophila

Metabolomic analysis of oxidative stress: Superoxide dismutase mutation and paraquat induced stress in Drosophila melanogaster

2017 ◽  
Vol 113 ◽  
pp. 323-334 ◽  
Author(s):  
Marney L. Doran ◽  
Jose M. Knee ◽  
Nan Wang ◽  
Teresa Z. Rzezniczak ◽  
Tony L. Parkes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Metabolomic Analysis ◽  
Induced Stress

Phenotypic consequences of copper-zinc superoxide dismutase overexpression in Drosophila melanogaster

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 867-872 ◽  
Author(s):  
Brian E. Staveley ◽  
John P. Phillips ◽  
Arthur J. Hilliker
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Ionizing Radiation ◽  
Life Span ◽  
Adult Life ◽  
Small Region ◽  
Laboratory Culture ◽  
Adult Life Span ◽  
Copper Zinc Superoxide Dismutase ◽  
Diploid Level

We report here the isolation of a tandem duplication of a small region of the third chomosome of Drosophila melanogaster containing the Cu–Zn superoxide dismutase (cSOD) gene. This duplication is associated with a dosage-dependent increase in cSOD activity. The biological consequences of hypermorphic levels of cSOD in genotypes carrying this duplication have been investigated under diverse conditions of oxygen stress imposed by acute exposure to ionizing radiation, chronic exposure to paraquat, and the normoxia of standard laboratory culture. We find that a 50% increase in cSOD activity above the normal diploid level confers increased resistance to ionizing radiation and, in contrast, confers decreased resistance to the superoxide-generating agent paraquat. The duplication is associated with a minor increase in adult life-span under conditions of normoxia. These results reveal important features of the biological function of cSOD within the context of the overall oxygen defense system of Drosophila.Key words: gene duplication, paraquat, ionizing radiation, life-span.

scholarly journals Phenotypic rescue by a bovine transgene in a Cu/Zn superoxide dismutase-null mutant of Drosophila melanogaster

1994 ◽  
Vol 14 (2) ◽  
pp. 1302-1307
Author(s):  
I Reveillaud ◽  
J Phillips ◽  
B Duyf ◽  
A Hilliker ◽  
A Kongpachith ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Life Span ◽  
Male Fertility ◽  
Transcriptional Control ◽  
Adult Life ◽  
P Element ◽  
Normal Male ◽  
Null Mutant ◽  
Adult Life Span

Null mutants for Cu/Zn superoxide dismutase (CuZnSOD) in Drosophila melanogaster are male sterile, have a greatly reduced adult life span, and are hypersensitive to paraquat. We have introduced a synthetic bovine CuZnSOD transgene under the transcriptional control of the D. melanogaster 5C actin promoter into a CuZnSOD-null mutant of D. melanogaster. This was carried out by P-element-mediated transformation of the Drosophila-bovine CuZnSOD transgene into a CuZnSOD+ recipient strain followed by genetic crossing of the transgene into a strain carrying the CuZnSOD-null mutation, cSODn108. The resulting transformants express bovine CuZnSOD exclusively to about 30% of normal Drosophila CuZnSOD levels. Expression of the Drosophila-bovine CuZnSOD transgene in the CuZnSOD-null mutant rescues male fertility and resistance to paraquat to apparently normal levels. However, adult life span is restored to only 30% of normal, and resistance to hyperoxia is 90% of that found in control flies. This striking differential restoration of pleiotropic phenotypes could be the result of a threshhold of CuZnSOD expression necessary for normal male fertility and resistance to the toxicity of paraquat or hyperoxia which is lower than the threshold required to sustain a normal adult life span. Alternatively, the differential rescue of fertility, resistance to active oxygen, and life span might indicate different cell-specific transcriptional requirements for these functions which are normally provided by the control elements of the native CuZnSOD gene but are only partly compensated for by the transcriptional control elements of the actin 5C promoter.

Dietary l-arginine accelerates pupation and promotes high protein levels but induces oxidative stress and reduces fecundity and life span in Drosophila melanogaster

2017 ◽  
Vol 188 (1) ◽  
pp. 37-55 ◽  
Author(s):  
Maria M. Bayliak ◽  
Maria P. Lylyk ◽  
Oksana V. Maniukh ◽  
Janet M. Storey ◽  
Kenneth B. Storey ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Life Span ◽  
High Protein ◽  
Protein Levels

Metal-based superoxide dismutase and catalase mimics reduce oxidative stress biomarkers and extend life span of Saccharomyces cerevisiae

2017 ◽  
Vol 474 (2) ◽  
pp. 301-315 ◽  
Author(s):  
Thales de P. Ribeiro ◽  
Fernanda L. Fonseca ◽  
Mariana D.C. de Carvalho ◽  
Rodrigo M. da C. Godinho ◽  
Fernando Pereira de Almeida ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Superoxide Dismutase ◽  
Life Span ◽  
Yeast Cells ◽  
Cell Integrity ◽  
Oxidative Stress Biomarkers ◽  
Chronological Aging ◽  
Stress Biomarkers

Aging is a natural process characterized by several biological changes. In this context, oxidative stress appears as a key factor that leads cells and organisms to severe dysfunctions and diseases. To cope with reactive oxygen species and oxidative-related damage, there has been increased use of superoxide dismutase (SOD)/catalase (CAT) biomimetic compounds. Recently, we have shown that three metal-based compounds {[Fe(HPClNOL)Cl2]NO3, [Cu(HPClNOL)(CH3CN)](ClO4)2 and Mn(HPClNOL)(Cl)2}, harboring in vitro SOD and/or CAT activities, were critical for protection of yeast cells against oxidative stress. In this work, treating Saccharomyces cerevisiae with these SOD/CAT mimics (25.0 µM/1 h), we highlight the pivotal role of these compounds to extend the life span of yeast during chronological aging. Evaluating lipid and protein oxidation of aged cells, it becomes evident that these mimics extend the life expectancy of yeast mainly due to the reduction in oxidative stress biomarkers. In addition, the treatment of yeast cells with these mimics regulated the amounts of lipid droplet occurrence, consistent with the requirement and protection of lipids for cell integrity during aging. Concerning SOD/CAT mimics uptake, using inductively coupled plasma mass spectrometry, we add new evidence that these complexes, besides being bioabsorbed by S. cerevisiae cells, can also affect metal homeostasis. Finally, our work presents a new application for these SOD/CAT mimics, which demonstrate a great potential to be employed as antiaging agents. Taken together, these promising results prompt future studies concerning the relevance of administration of these molecules against the emerging aging-related diseases such as Parkinson's, Alzheimer's and Huntington's.

Overexpression of Mn-Containing Superoxide Dismutase in Transgenic Drosophila melanogaster

1999 ◽  
Vol 371 (2) ◽  
pp. 260-269 ◽  
Author(s):  
Robin J Mockett ◽  
William C Orr ◽  
Jennifer J Rahmandar ◽  
Judith J Benes ◽  
Svetlana N Radyuk ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Drosophila Melanogaster ◽  
Transgenic Drosophila
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