scholarly journals Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

2008 ◽  
Vol 28 (11) ◽  
pp. 3882-3882
Author(s):  
Yoshitaka Miyagawa ◽  
Hajime Okita ◽  
Hideki Nakaijima ◽  
Yasuomi Horiuchi ◽  
Ban Sato ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Inducible Expression ◽  
Mesenchymal Progenitor Cells ◽  
Ets Proteins

scholarly journals Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

2008 ◽  
Vol 28 (7) ◽  
pp. 2125-2137 ◽  
Author(s):  
Yoshitaka Miyagawa ◽  
Hajime Okita ◽  
Hideki Nakaijima ◽  
Yasuomi Horiuchi ◽  
Ban Sato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Ectopic Expression ◽  
Chromosomal Translocations ◽  
Mesenchymal Progenitor Cells ◽  
Pediatric Tumor ◽  
Ets Proteins ◽  
Polygonal Cell ◽  
Transcription Factor Genes ◽  
Ets Transcription Factor

ABSTRACT Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in the onset and progression of EFT. However, the mechanisms responsible for the EWS/ETS-mediated onset remain unclear. Here we report the establishment of a tetracycline-controlled EWS/ETS-inducible system in human bone marrow-derived mesenchymal progenitor cells (MPCs). Ectopic expression of both EWS/FLI1 and EWS/ERG proteins resulted in a dramatic change of morphology, i.e., from a mesenchymal spindle shape to a small round-to-polygonal cell, one of the characteristics of EFT. EWS/ETS also induced immunophenotypic changes in MPCs, including the disappearance of the mesenchyme-positive markers CD10 and CD13 and the up-regulation of the EFT-positive markers CD54, CD99, CD117, and CD271. Furthermore, a prominent shift from the gene expression profile of MPCs to that of EFT was observed in the presence of EWS/ETS. Together with the observation that EWS/ETS enhances the ability of cells to invade Matrigel, these results suggest that EWS/ETS proteins contribute to alterations of cellular features and confer an EFT-like phenotype to human MPCs.

Inducible Expression of Neurotrophic Factors by Mesenchymal Progenitor Cells Derived from Traumatically Injured Human Muscle

2011 ◽  
Vol 51 (2) ◽  
pp. 128-136 ◽  
Author(s):  
Jamie D. Bulken-Hoover ◽  
Wesley M. Jackson ◽  
Youngmi Ji ◽  
Jared A. Volger ◽  
Rocky S. Tuan ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neurotrophic Factors ◽  
Inducible Expression ◽  
Human Muscle ◽  
Mesenchymal Progenitor Cells

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal progenitor cells with myogenic and neuronal properties

2007 ◽  
Vol 313 (5) ◽  
pp. 1008-1023 ◽  
Author(s):  
Mitsutaka Shiota ◽  
Toshio Heike ◽  
Munetada Haruyama ◽  
Shiro Baba ◽  
Atsunori Tsuchiya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Isolation And Characterization

scholarly journals In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid

2014 ◽  
Vol 29 (4) ◽  
pp. 457-469 ◽  
Author(s):  
Federica Riva ◽  
Claudia Omes ◽  
Roberto Bassani ◽  
Rossella E Nappi ◽  
Giuliano Mazzini ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Progenitor Cells ◽  
Follicular Fluid ◽  
Culture System ◽  
Mesenchymal Progenitor Cells ◽  
In Vitro Culture System ◽  
Ovarian Follicular Fluid

Response of Cells on Surface-Induced Nanopatterns:  Fibroblasts and Mesenchymal Progenitor Cells

2007 ◽  
Vol 8 (5) ◽  
pp. 1530-1540 ◽  
Author(s):  
Hwei Ling Khor ◽  
Yujun Kuan ◽  
Hildegard Kukula ◽  
Kaoru Tamada ◽  
Wolfgang Knoll ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells
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