scholarly journals Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

2008 ◽  
Vol 28 (7) ◽  
pp. 2125-2137 ◽  
Author(s):  
Yoshitaka Miyagawa ◽  
Hajime Okita ◽  
Hideki Nakaijima ◽  
Yasuomi Horiuchi ◽  
Ban Sato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Ectopic Expression ◽  
Chromosomal Translocations ◽  
Mesenchymal Progenitor Cells ◽  
Pediatric Tumor ◽  
Ets Proteins ◽  
Polygonal Cell ◽  
Transcription Factor Genes ◽  
Ets Transcription Factor

ABSTRACT Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in the onset and progression of EFT. However, the mechanisms responsible for the EWS/ETS-mediated onset remain unclear. Here we report the establishment of a tetracycline-controlled EWS/ETS-inducible system in human bone marrow-derived mesenchymal progenitor cells (MPCs). Ectopic expression of both EWS/FLI1 and EWS/ERG proteins resulted in a dramatic change of morphology, i.e., from a mesenchymal spindle shape to a small round-to-polygonal cell, one of the characteristics of EFT. EWS/ETS also induced immunophenotypic changes in MPCs, including the disappearance of the mesenchyme-positive markers CD10 and CD13 and the up-regulation of the EFT-positive markers CD54, CD99, CD117, and CD271. Furthermore, a prominent shift from the gene expression profile of MPCs to that of EFT was observed in the presence of EWS/ETS. Together with the observation that EWS/ETS enhances the ability of cells to invade Matrigel, these results suggest that EWS/ETS proteins contribute to alterations of cellular features and confer an EFT-like phenotype to human MPCs.

scholarly journals Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment

2018 ◽  
Vol 19 (9) ◽  
pp. 2549 ◽  
Author(s):  
Luisa Brandt ◽  
Susanna Schubert ◽  
Patrick Scheibe ◽  
Walter Brehm ◽  
Jan Franzen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Culture Conditions ◽  
Mesenchymal Progenitor Cells ◽  
Blood Leukocytes ◽  
Inflammatory Conditions ◽  
Tenogenic Differentiation ◽  
Adipose Derived Stromal Cells ◽  
Factor Α ◽  
The Impact

Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expression. This included decreased expression of the tenogenic transcription factor scleraxis by an inflammatory environment throughout culture conditions. The data demonstrate that ASC tenogenic properties are compromised in an inflammatory environment, with relevance to their possible mechanisms of action in acute tendon disease.

Plasmid vectors harboring cellular promoters can induce prolonged gene expression in hematopoietic and mesenchymal progenitor cells

2005 ◽  
Vol 332 (2) ◽  
pp. 518-523 ◽  
Author(s):  
Hyang-Min Byun ◽  
Dongchul Suh ◽  
Youngsin Jeong ◽  
Hyung Seok Wee ◽  
Jung Mogg Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Mesenchymal Progenitor Cells ◽  
Plasmid Vectors

scholarly journals Rapid Production and Genetic Stability of Human Mesenchymal Progenitor Cells Derived from Human Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells

2021 ◽  
Vol 22 (17) ◽  
pp. 9238
Author(s):  
Soo Kyung Jung ◽  
Jeoung Eun Lee ◽  
Chang Woo Lee ◽  
Sung Han Shim ◽  
Dong Ryul Lee
Keyword(s):  
Gene Expression ◽  
Somatic Cell ◽  
Progenitor Cells ◽  
Genetic Stability ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Mesenchymal Progenitor Cells ◽  
Direct Differentiation Method ◽  
Direct Differentiation ◽  
Differentiation Method

Pluripotent stem cell-derived mesenchymal progenitor cells (PSC-MPCs) are primarily derived through two main methods: three-dimensional (3D) embryoid body-platform (EB formation) and the 2D direct differentiation method. We recently established somatic cell nuclear transfer (SCNT)-PSC lines and showed their stemness. In the present study, we produced SCNT-PSC-MPCs using a novel direct differentiation method, and the characteristics, gene expression, and genetic stability of these MPCs were compared with those derived through EB formation. The recovery and purification of SCNT-PSC-Direct-MPCs were significantly accelerated compared to those of the SCNT-PSC-EB-MPCs, but both types of MPCs expressed typical surface markers and exhibited similar proliferation and differentiation potentials. Additionally, the analysis of gene expression patterns using microarrays showed very similar patterns. Moreover, array CGH analysis showed that both SCNT-PSC-Direct-MPCs and SCNT-PSC-EB-MPCs exhibited no significant differences in copy number variation (CNV) or single-nucleotide polymorphism (SNP) frequency. These results indicate that SCNT-PSC-Direct-MPCs exhibited high genetic stability even after rapid differentiation into MPCs, and the rate at which directly derived MPCs reached a sufficient number was higher than that of MPCs derived through the EB method. Therefore, we suggest that the direct method of differentiating MPCs from SCNT-PSCs can improve the efficacy of SCNT-PSCs applied to allogeneic transplantation.

scholarly journals Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells

2008 ◽  
Vol 28 (11) ◽  
pp. 3882-3882
Author(s):  
Yoshitaka Miyagawa ◽  
Hajime Okita ◽  
Hideki Nakaijima ◽  
Yasuomi Horiuchi ◽  
Ban Sato ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Inducible Expression ◽  
Mesenchymal Progenitor Cells ◽  
Ets Proteins

scholarly journals Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts

2021 ◽  
Vol 22 (2) ◽  
pp. 796
Author(s):  
Anna-Klara Amler ◽  
Patrick H. Dinkelborg ◽  
Domenic Schlauch ◽  
Jacob Spinnen ◽  
Stefan Stich ◽  
...  
Keyword(s):  
Gene Expression ◽  
Minimally Invasive ◽  
Progenitor Cells ◽  
Alveolar Bone ◽  
Bone Grafts ◽  
Osteogenic Potential ◽  
3D Bioprinting ◽  
Mesenchymal Progenitor Cells ◽  
Cell Source ◽  
Autologous Bone

Reconstruction of segmental bone defects by autologous bone grafting is still the standard of care but presents challenges including anatomical availability and potential donor site morbidity. The process of 3D bioprinting, the application of 3D printing for direct fabrication of living tissue, opens new possibilities for highly personalized tissue implants, making it an appealing alternative to autologous bone grafts. One of the most crucial hurdles for the clinical application of 3D bioprinting is the choice of a suitable cell source, which should be minimally invasive, with high osteogenic potential, with fast, easy expansion. In this study, mesenchymal progenitor cells were isolated from clinically relevant human bone biopsy sites (explant cultures from alveolar bone, iliac crest and fibula; bone marrow aspirates; and periosteal bone shaving from the mastoid) and 3D bioprinted using projection-based stereolithography. Printed constructs were cultivated for 28 days and analyzed regarding their osteogenic potential by assessing viability, mineralization, and gene expression. While viability levels of all cell sources were comparable over the course of the cultivation, cells obtained by periosteal bone shaving showed higher mineralization of the print matrix, with gene expression data suggesting advanced osteogenic differentiation. These results indicate that periosteum-derived cells represent a highly promising cell source for translational bioprinting of bone tissue given their superior osteogenic potential as well as their minimally invasive obtainability.

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