scholarly journals Study Comparing Human Papillomavirus (HPV) Real-Time Multiplex PCR and Hybrid Capture II INNO-LiPA v2 HPV Genotyping PCR Assays

2009 ◽  
Vol 47 (7) ◽  
pp. 2106-2113 ◽  
Author(s):  
T. Iftner ◽  
L. Germ ◽  
R. Swoyer ◽  
S. K. Kjaer ◽  
J. G. Breugelmans ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Multiplex Pcr ◽  
Real Time ◽  
Hybrid Capture ◽  
Hpv Genotyping ◽  
Pcr Assays

scholarly journals Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with INNO-LiPA HPV Genotyping Extra Assay

2010 ◽  
Vol 49 (5) ◽  
pp. 1907-1912 ◽  
Author(s):  
E. A. Else ◽  
R. Swoyer ◽  
Y. Zhang ◽  
F. J. Taddeo ◽  
J. T. Bryan ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Real Time ◽  
Hpv Genotyping ◽  
Pcr Assays

scholarly journals Rapid Multiplex PCR and Real-Time TaqMan PCR Assays for Detection of Salmonella enterica and the Highly Virulent Serovars Choleraesuis and Paratyphi C

2008 ◽  
Vol 46 (12) ◽  
pp. 4018-4022 ◽  
Author(s):  
D. F. Woods ◽  
F. J. Reen ◽  
D. Gilroy ◽  
J. Buckley ◽  
J. G. Frye ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Salmonella Enterica ◽  
Taqman Pcr ◽  
Pcr Assays ◽  
Highly Virulent

A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV Genotyping Extra assay for the detection and typing of human papillomavirus

2013 ◽  
Vol 187 (1) ◽  
pp. 166-171 ◽  
Author(s):  
M.I. Micalessi ◽  
G.A. Boulet ◽  
A. Vorsters ◽  
K. De Wit ◽  
G. Jannes ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Real Time ◽  
Real Time Pcr ◽  
Hpv Genotyping

scholarly journals Detection of human papillomavirus in pterygium and conjunctival papilloma by hybrid capture II and PCR assays

Eye ◽  
2008 ◽  
Vol 22 (11) ◽  
pp. 1442-1445 ◽  
Author(s):  
Y Takamura ◽  
E Kubo ◽  
S Tsuzuki ◽  
Y Akagi
Keyword(s):  
Human Papillomavirus ◽  
Hybrid Capture ◽  
Pcr Assays

Molecular Methods for a Correct Diagnosis of Multiple HPV Infections and Clinical Implications for Vaccine

2011 ◽  
Vol 21 (3) ◽  
pp. 545-550 ◽  
Author(s):  
Andrea Tinelli ◽  
Giuseppe Leo ◽  
Domenico Dell'Edera ◽  
Fabio Storelli ◽  
Maria Maddalena Galante ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Real Time ◽  
Sequence Homology ◽  
Direct Sequencing ◽  
Chain Reaction ◽  
Hpv 16 ◽  
Hpv Genotyping ◽  
Polymerase Chain ◽  
Hpv 18

IntroductionThe human papillomavirus (HPV) family is characterized by minimal genotypic differences corresponding to different virus types. The aim of this study was to detect the HPV coinfections and the inner genotype in a series of 336 cervical-vaginal samples.MethodsA total of 336 cervical-vaginal samples were taken from 2007 to 2009 using specific molecular techniques such as molecular sequencing and hybridizations. The genome amplification of the L1 open reading frame was analyzed by real-time polymerase chain reaction; direct sequencing was performed by SYBR green fluorescent molecule and degenerate primers MY09 and MY11. The HPV genotyping was accomplished via oligonucleotide probe hybridization. The phylogenetic correlations in coinfections were analyzed by sequence homology of the L1 genomic region. Identified genotypes were then compared.ResultsHuman papillomavirus positivity was observed in 125 cases (37.2%), with 21 cases (16.8%) of HPV presence in coinfections. Coinfections involved HPV 16 genotype (8 cases) and HPV 18 (5 cases). The HPV 16 infection was mainly associated with genotypes with a lower-than-broad sequence homology, so the HPV 18 was linked to genotypes represented in the opposite phylogenetic tree.ConclusionsThe combined and steady use of diagnostic procedures, such as real-time polymerase chain reaction, molecular hybridization, direct sequencing, and HPV genotyping test, allow accurate diagnosis of monoinfections and coinfections. This may faciliate the development of specific viral tests and prophylactic anti-HPV vaccines.

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