scholarly journals Detection of human papillomavirus in pterygium and conjunctival papilloma by hybrid capture II and PCR assays

Eye ◽  
2008 ◽  
Vol 22 (11) ◽  
pp. 1442-1445 ◽  
Author(s):  
Y Takamura ◽  
E Kubo ◽  
S Tsuzuki ◽  
Y Akagi
Keyword(s):  
Human Papillomavirus ◽  
Hybrid Capture ◽  
Pcr Assays

scholarly journals Comparison of Human Papillomavirus Detection and Typing by Cycle Sequencing, Line Blotting, and Hybrid Capture

2000 ◽  
Vol 38 (2) ◽  
pp. 651-655 ◽  
Author(s):  
Suzanne D. Vernon ◽  
Elizabeth R. Unger ◽  
Deon Williams
Keyword(s):  
Human Papillomavirus ◽  
Gel Electrophoresis ◽  
The Other ◽  
Pcr Assay ◽  
Cycle Sequencing ◽  
Cross Hybridization ◽  
Hybrid Capture ◽  
Capture Assay ◽  
Hpv Types ◽  
Pcr Assays

We compared the results of human papillomavirus (HPV) detection and typing from 781 cervical samples assayed by three methods: L1 consensus PCR followed by cycle sequencing, L1 consensus PCR with biotinylated primers followed by hybridization to a line blot, and Hybrid Capture assay. Both PCR assays used L1 consensus PCR with primers MY09 and MY11. We evaluated the amplification efficiencies of both PCR assays and also compared the specific HPV types detected by each method. The samples positive by the Hybrid Capture assay were compared to the specific types detected by the PCR-based assays. The concordance between the two PCR assays in producing an HPV amplicon visible by gel electrophoresis or in detecting any HPV type was moderate: kappa values were 0.61 (95% confidence interval [CI] = 0.56 to 0.67) and 0.51 (95% CI = 0.46 to 0.58), respectively. The McNemar test for correlated proportions indicated that biotinylated PCR was less likely to produce a band (P = 0.001) and to detect an HPV type (P = 0.001) than the other PCR assay. In comparing the Hybrid Capture assay results with the HPV types detected by the PCR-based assays, we found that positivity by the Hybrid Capture assay for a number of samples may be due to cross-hybridization with HPV types not included in the Hybrid Capture assay probe cocktails.

scholarly journals Study Comparing Human Papillomavirus (HPV) Real-Time Multiplex PCR and Hybrid Capture II INNO-LiPA v2 HPV Genotyping PCR Assays

2009 ◽  
Vol 47 (7) ◽  
pp. 2106-2113 ◽  
Author(s):  
T. Iftner ◽  
L. Germ ◽  
R. Swoyer ◽  
S. K. Kjaer ◽  
J. G. Breugelmans ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Multiplex Pcr ◽  
Real Time ◽  
Hybrid Capture ◽  
Hpv Genotyping ◽  
Pcr Assays

Anal Human Papillomavirus Testing with Digeneʼs Hybrid Capture 2 Using Two Different Sampling Methods

2008 ◽  
Vol 51 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Florian Roka ◽  
Julia Roka ◽  
Andreas Trost ◽  
Horst Schalk ◽  
Christian Zagler ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Sampling Methods ◽  
Hybrid Capture ◽  
Human Papillomavirus Testing ◽  
Hybrid Capture 2

scholarly journals Low prevalence of high risk human papillomavirus in normal oral mucosa by hybrid capture 2

2008 ◽  
Vol 39 (1) ◽  
Author(s):  
Maria del Refugio González-Losa ◽  
Luis Manzano-Cabrera ◽  
Florencio Rueda-Gordillo ◽  
Sandra E. Hernández-Solís ◽  
Marylin Puerto-Solís
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Oral Mucosa ◽  
Hybrid Capture ◽  
Normal Oral Mucosa ◽  
Hybrid Capture 2 ◽  
Low Prevalence

scholarly journals Prevalence of Anal Human Papillomavirus (HPV) and Performance of Cepheid Xpert and Hybrid Capture 2 (hc2) HPV Assays in South African HIV-Infected Women

2017 ◽  
Vol 148 (2) ◽  
pp. 148-153 ◽  
Author(s):  
Zizipho Z. A. Mbulawa ◽  
Timothy Wilkin ◽  
Bridgette J. Goeieman ◽  
Eefje Jong ◽  
Pamela Michelow ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
South African ◽  
Hybrid Capture ◽  
Hybrid Capture 2 ◽  
And Performance ◽  
Hpv Assays
Sign in / Sign up

Export Citation Format

Share Document