Detection of aacA-aphD, qacEδ1, marA, floR, and tetA genes from multidrug-resistant bacteria: Comparative analysis of real-time multiplex PCR assays using EvaGreen® and SYBR® Green I dyes

2011 ◽  
Vol 25 (2-3) ◽  
pp. 78-86 ◽  
Author(s):  
Saeed A. Khan ◽  
Kidon Sung ◽  
Mohamed S. Nawaz
Keyword(s):  
Comparative Analysis ◽  
Multiplex Pcr ◽  
Real Time ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Pcr Assays

scholarly journals Next-Generation Epidemiology: Using Real-Time Core Genome Multilocus Sequence Typing To Support Infection Control Policy

2016 ◽  
Vol 54 (12) ◽  
pp. 2850-2853 ◽  
Author(s):  
John P. Dekker ◽  
Karen M. Frank
Keyword(s):  
Infection Control ◽  
Real Time ◽  
Core Genome ◽  
Hospital Setting ◽  
Control Policy ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Substantial Morbidity ◽  
Multilocus Sequencing

Multidrug-resistant bacteria are responsible for substantial morbidity and mortality worldwide. Tracking the nosocomial spread of resistant bacteria is critical to infection control. Mellmann et al. (J. Clin. Microbiol. 54:2874–2881, 2016, http://dx.doi.org/10.1128/JCM.00790-16 ) have described prospective whole-genome sequencing with core genome multilocus sequencing typing (cgMLST) analysis for real-time surveillance and have addressed the practical aspects of implementing this type of operation in the hospital setting.

scholarly journals Rapid Detection of Carbapenemase Genes (Bla-Vim And Bla-Imp) By Multiplex Pcr in Gram-Negative Clinical Isolates

2020 ◽  
Author(s):  
Mehraj Ansari ◽  
Kulraj Rai ◽  
Ganesh Rai ◽  
Subhas Aryal ◽  
Shiba Rai ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Gram Negative ◽  
Positive Results

Abstract BackgroundCarbapenems have been the choice of antibiotics for the treatment of infections caused by multidrug-resistant bacteria. However, during recent years, carbapenems resistant bacteria have emerged significantly. The main objective of this study was to determine the prevalence of carbapenemase (bla-VIM and bla-IMP) producing isolates among Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii.ResultsOf the total 1,151 clinical samples, 253 (22.0%) showed growth positive. Of them, 226 (89.3%) were identified as members of Enterobacteriaceae, P. aeruginosa and A. baumannii. Among the 226 isolates, 106 (46.9%) were multidrug-resistant. Of the 106, 97 (91.5%) isolates showed resistance to at least one of the carbapenem used. Among the 97 isolates, 67 (69.1%) showed MHT positive results. bla-VIM and bla-IMP were detected in 40 and 38 isolates, respectively.ConclusionThis study determined the higher prevalence of MDR and carbapenem resistance among Enterobacteriaceae, P. aeruginosa and A. baumannii as detected by the presence of bla-VIM and bla-IMP genes.Keywords: Carbapenems, Carbapenemase, Modified Hodge Test, bla-VIM, bla-IMP

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