scholarly journals Antimicrobial Susceptibility Profiles and Molecular Typing of Campylobacter jejuni and Campylobacter coli Isolates from Ducks in South Korea

2014 ◽  
Vol 80 (24) ◽  
pp. 7604-7610 ◽  
Author(s):  
Bai Wei ◽  
Se-Yeoun Cha ◽  
Min Kang ◽  
Jae-Hee Roh ◽  
Hye-Suk Seo ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Multidrug Resistant ◽  
Human Infection ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Campylobacter Coli ◽  
Content Type ◽  
Food Borne ◽  
Production Animals ◽  
Susceptibility Profiles

ABSTRACTCampylobacteris a food-borne zoonotic pathogen that causes human gastroenteritis worldwide.Campylobacterbacteria are commensal in the intestines of many food production animals, including ducks and chickens. The objective of the study was to determine the prevalence ofCampylobacterspecies in domestic ducks, and the agar dilution method was used to determine resistance of the isolates to eight antibiotics. In addition, multilocus sequence typing (MLST) was performed to determine the sequence types (STs) of selectedCampylobacterisolates. Between May and September 2012, 58 duck farms were analyzed, and 56 (96.6%) were positive forCampylobacter. Among the isolates, 82.1% wereCampylobacter jejuni, 16.1% wereC. coli, and one was unidentified by PCR. Of the 46C. jejuniisolates, 87.0%, 10.9%, and 21.7% were resistant to ciprofloxacin, erythromycin, and azithromycin, respectively. Among theC. coliisolates, all 9 strains were resistant to ampicillin, and 77.8% and 33.3% were resistant to ciprofloxacin and azithromycin, respectively. The majority of theCampylobacterisolates were classified as multidrug resistant. Twenty-eight STs were identified, including 20 STs forC. jejuniand 8 STs forC. coli. The most common clonal complexes inC. jejuniwere the ST-21 complex and the ST-45 complex, while the ST-828 complex predominated inC. coli. The majority of isolates were of STs noted in ducks and humans from earlier studies, along with seven STs previously associated only with human disease. These STs overlapped between duck and human isolates, indicating thatCampylobacterisolates from ducks should be considered potential sources of human infection.

scholarly journals Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

2013 ◽  
Vol 79 (12) ◽  
pp. 3875-3878 ◽  
Author(s):  
Sonja Kittl ◽  
Bożena M. Korczak ◽  
Lilian Niederer ◽  
Andreas Baumgartner ◽  
Sabina Buettner ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Human Infection ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Production Chain ◽  
Content Type ◽  
Resistance Patterns ◽  
Retail Meat ◽  
Retail Chicken Meat ◽  
Antibiotic Resistance Patterns

ABSTRACTMultilocus sequence typing (MLST) and antibiotic resistance patterns ofCampylobacter jejuniandCampylobacter colifrom retail chicken meat showed high overlap with isolates collected at slaughterhouses, indicating little selection along the production chain. They also showed significant common sequence types with human clinical isolates, revealing chicken meat as a likely source for human infection.

scholarly journals Antimicrobial Susceptibilities of Clostridium difficile Isolates from 12 Asia-Pacific Countries in 2014 and 2015

2020 ◽  
Vol 64 (7) ◽  
Author(s):  
Tanya Lew ◽  
Papanin Putsathit ◽  
Kyung Mok Sohn ◽  
Yuan Wu ◽  
Kentaro Ouchi ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Asia Pacific ◽  
Fluoroquinolone Resistance ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Content Type ◽  
Amoxicillin Clavulanate ◽  
Ongoing Surveillance ◽  
Susceptibility Profiles ◽  
Hospital Acquired

ABSTRACT Clostridium (Clostridioides) difficile causes toxin-mediated diarrhea and pseudomembranous colitis, primarily among hospital inpatients. Outbreaks of C. difficile infection (CDI) have been caused by strains with acquired antimicrobial resistance, particularly fluoroquinolone resistance, including C. difficile ribotype (RT) 027 in North America and Europe and RT 017, the most common strain in Asia. Despite being the most common cause of hospital-acquired infection in high-income countries, and frequent misuse of antimicrobials in Asia, little is known about CDI in the Asia-Pacific region. We aimed to determine the antimicrobial susceptibility profiles of a collection of C. difficile isolates from the region. C. difficile isolates (n = 414) from a 2014 study of 13 Asia-Pacific countries were tested for susceptibility to moxifloxacin, amoxicillin-clavulanate, erythromycin, clindamycin, rifaximin, metronidazole, vancomycin, and fidaxomicin according to the Clinical and Laboratory Standards Institute’s agar dilution method. All isolates were susceptible to metronidazole, vancomycin, amoxicillin-clavulanate, and fidaxomicin. Moxifloxacin resistance was detected in all countries except Australia, all RT 369 and QX 239 strains, and 92.7% of RT 018 and 70.6% of RT 017 strains. All C. difficile RT 012, 369, and QX 239 strains were also resistant to erythromycin and clindamycin. Rifaximin resistance was common in RT 017 strains only (63.2%) and was not detected in Australian, Japanese, or Singaporean isolates. In conclusion, antimicrobial susceptibility of C. difficile varied by strain type and by country. Multiresistance was common in emerging RTs 369 and QX 239 and the most common strain in Asia, RT 017. Ongoing surveillance is clearly warranted.

scholarly journals Antimicrobial Susceptibilities of Campylobacter Strains Isolated from Finnish Subjects Infected Domestically or from Those Infected Abroad

2003 ◽  
Vol 47 (1) ◽  
pp. 102-105 ◽  
Author(s):  
Hilpi Rautelin ◽  
Antti Vierikko ◽  
Marja-Liisa Hänninen ◽  
Martti Vaara
Keyword(s):  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Campylobacter Coli ◽  
Antimicrobial Susceptibilities ◽  
Agar Dilution ◽  
Stool Samples

ABSTRACT The in vitro susceptibilities of 678 Campylobacter jejuni and Campylobacter coli strains isolated from stool samples of the same number of Finnish subjects were studied. A total of 523 patients, representing inhabitants from throughout Finland, had not traveled abroad within the 2 weeks prior to becoming ill, whereas 155 persons had presumably acquired their infections abroad. The antimicrobial agents studied were erythromycin, ciprofloxacin, levofloxacin, trovafloxacin, and moxifloxacin. The MICs of these antimicrobial agents were determined by the agar dilution method. The growth of all domestic isolates was inhibited by erythromycin at concentrations of 4 μg/ml, and for these isolates the fluoroquinolone MICs at which 90% of isolates are inhibited (MIC90s) ranged from 0.06 to 0.5 μg/ml. For the foreign isolates, the erythromycin MIC90 was still low (4 μg/ml), but their susceptibilities to fluoroquinolones were clearly reduced (MIC90s, 8 to 64 μg/ml). Of the four different fluoroquinolones studied, ciprofloxacin was the least active (MIC90, 64 μg/ml).

scholarly journals Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories

2021 ◽  
Vol 12 ◽  
Author(s):  
José F. Cobo-Díaz ◽  
Paloma González del Río ◽  
Avelino Álvarez-Ordóñez
Keyword(s):  
Campylobacter Jejuni ◽  
Tetracycline Resistance ◽  
Multidrug Resistant ◽  
Host Specialization ◽  
Campylobacter Coli ◽  
Geographical Differences ◽  
Clear Trend ◽  
Antimicrobial Resistance Gene ◽  
Public Repositories ◽  
Production Animals

Campylobacter spp. are the most frequent agent of human gastroenteritis worldwide, and the spread of multidrug-resistant strains makes the clinical treatment difficult. The current study presents the resistome analysis of 39,798 Campylobacter jejuni and 11,920 Campylobacter coli genomes available in public repositories. Determinants of resistance to β-lactams (Be) and tetracyclines (Te) were the most frequent for both species, with resistance to quinolones (Qu) as the third most important on C. jejuni and to aminoglycosides (Am) on C. coli. Moreover, resistance to Te, Qu, and Am was frequently found in co-occurrence with resistance to other antibiotic families. Geographical differences on clonal complexes distribution were found for C. jejuni and on resistome genotypes for both C. jejuni and C. coli species. Attending to the resistome patterns by isolation source, three main clusters of genomes were found on C. jejuni genomes at antimicrobial resistance gene level. The first cluster was formed by genomes from human, food production animals (e.g., sheep, cow, and chicken), and food (e.g., dairy products) isolates. The higher incidence of tet(O), associated with tetracycline resistance, and the gyrA (T86I) single-nucleotide polymorphism (SNP), associated with quinolone resistance, among genomes from this cluster could be due to the intense use of these antibiotics in veterinary and human clinical settings. Similarly, a high incidence of tet(O) genes of C. coli genomes from pig, cow, and turkey was found. Moreover, the cluster based on resistome patterns formed by C. jejuni and C. coli genomes of human, turkey, and chicken origin is in agreement with previous observations reporting chicken or poultry-related environments as the main source of human campylobacteriosis infections. Most clonal complexes (CCs) associated with chicken host specialization (e.g., ST-354, ST-573, ST-464, and ST-446) were the CCs with the highest prevalence of determinants of resistance to Be, Qu, and Te. Finally, a clear trend toward an increase in the occurrence of Te and Qu resistance determinants on C. jejuni, linked to the spread of the co-occurrence of the blaOXA–61 and tet(O)-tet(O/W/O) genes and the gyrA (T86I) SNP, was found from 2001 to date in Europe.

scholarly journals Narrow-Spectrum Inhibitors of Campylobacter jejuni Flagellar Expression and Growth

2015 ◽  
Vol 59 (7) ◽  
pp. 3880-3886 ◽  
Author(s):  
Jeremiah G. Johnson ◽  
Caroline Yuhas ◽  
Thomas J. McQuade ◽  
Martha J. Larsen ◽  
Victor J. DiRita
Keyword(s):  
Small Molecules ◽  
Campylobacter Jejuni ◽  
Campylobacter Coli ◽  
Content Type ◽  
Food Borne ◽  
Food Borne Illness ◽  
Inhibitor Screen

ABSTRACTCampylobacter jejuniis a major cause of food-borne illness due to its ability to reside within the gastrointestinal tracts of chickens. Multiple studies have identified the flagella ofC. jejunias a major determinant of chicken colonization. An inhibitor screen of approximately 147,000 small molecules was performed to identify compounds that are able to inhibit flagellar expression in a reporter strain ofC. jejuni. Several compounds that modestly inhibited motility of wild-typeC. jejuniin standard assays were identified, as were a number of small molecules that robustly inhibitedC. jejunigrowth,in vitro. Examination of similar bacterial screens found that many of these small molecules inhibited only the growth ofC. jejuni. Follow-up assays demonstrated inhibition of other strains ofC. jejuniandCampylobacter colibut no inhibition of the closely relatedHelicobacter pylori. The compounds were determined to be bacteriostatic and nontoxic to eukaryotic cells. Preliminary results from a day-of-hatch chick model of colonization suggest that at least one of the compounds demonstrates promise for reducingCampylobactercolonization loadsin vivo, although further medicinal chemistry may be required to enhance bioavailability.

scholarly journals Complete Genome Sequences of Plasmid-Bearing Multidrug-Resistant Campylobacter jejuni and Campylobacter coli Strains with Type VI Secretion Systems, Isolated from Retail Turkey and Pork

2017 ◽  
Vol 5 (47) ◽  
Author(s):  
Daya Marasini ◽  
Mohamed K. Fakhr
Keyword(s):  
Campylobacter Jejuni ◽  
Complete Genome ◽  
Multidrug Resistant ◽  
Campylobacter Coli ◽  
Type Vi Secretion System ◽  
Secretion Systems ◽  
Genome Sequences ◽  
Content Type ◽  
Type Vi Secretion ◽  
Antimicrobial Resistance Genes

ABSTRACT We report the complete genome sequences of multidrug-resistant Campylobacter jejuni and Campylobacter coli isolated from retail turkey and pork, respectively. The chromosomes of these two isolates contained type VI secretion system genes. The two isolates also harbored large plasmids with antimicrobial resistance genes possibly contributing to their multidrug resistance.

scholarly journals Comparison of phenotypic and WGS-derived antimicrobial resistance profiles of Campylobacter jejuni and Campylobacter coli isolated from cases of diarrhoeal disease in England and Wales, 2015–16

2020 ◽  
Vol 75 (4) ◽  
pp. 883-889 ◽  
Author(s):  
Anaïs Painset ◽  
Martin Day ◽  
Michel Doumith ◽  
Jonathan Rigby ◽  
Claire Jenkins ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Diarrhoeal Disease ◽  
Culture Collection ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Campylobacter Coli ◽  
England And Wales ◽  
Phenotypic Resistance ◽  
Agar Dilution

Abstract Objectives To compare and evaluate phenotypic and genotypic methods for the detection of antimicrobial resistance (AMR) in Campylobacter jejuni and Campylobacter coli in England and Wales. Methods WGS data from 528 isolates of Campylobacter spp. (452 C. jejuni and 76 C. coli) from human (494), food (21) and environmental (2) sources, collected between January 2015 and December 2016, and from the PHE culture collection (11) were mapped to genes known to be associated with phenotypic resistance to antimicrobials in the genus. Phenotypic antibiotic susceptibility (erythromycin, ciprofloxacin, tetracycline, gentamicin and streptomycin) testing using an in-agar dilution method was performed on all isolates. Results Concordance between phenotypic resistance and the presence of corresponding AMR determinants was 97.5% (515/528 isolates). Only 13 out of 528 isolates (10 C. jejuni and 3 C. coli) had discordant interpretations for at least one of the five antibiotics tested, equating to a total of 15 (0.6%) discrepancies out of 2640 isolate/antimicrobial combinations. Seven discrepant results were genotypically resistant but phenotypically susceptible (major errors) and eight discrepant results were genotypically susceptible but phenotypically resistant (very major errors). Conclusions The use of this bioinformatics approach for predicting AMR from WGS data for routine public health surveillance is a reliable method for real-time monitoring of changing AMR patterns in isolates of C. jejuni and C. coli.

scholarly journals Strain-Specific Differences in Survival of Campylobacter spp. in Naturally Contaminated Turkey Feces and Water

2019 ◽  
Vol 85 (22) ◽  
Author(s):  
Lesley Good ◽  
William G. Miller ◽  
Jeffrey Niedermeyer ◽  
Jason Osborne ◽  
Robin M. Siletzky ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Foodborne Pathogens ◽  
Multidrug Resistant ◽  
Campylobacter Coli ◽  
Poultry Production ◽  
Content Type ◽  
Poultry Products ◽  
Water Suspensions ◽  
Different Strains ◽  
Commercial Turkey

ABSTRACT Campylobacter jejuni and Campylobacter coli are leading causes of human foodborne illness, with poultry as a major vehicle. Turkeys are frequently colonized with Campylobacter, but little is known about Campylobacter survival in turkey feces, even though fecal droppings are major vehicles for Campylobacter within-flock transmission as well as for environmental dissemination. Our objective was to examine survival of Campylobacter, including different strains, in freshly excreted feces from naturally colonized commercial turkey flocks and in suspensions of turkey feces in water from the turkey house. Fecal and water suspensions were stored at 4°C, and Campylobacter populations were enumerated on selective media at 48-h intervals. C. jejuni and C. coli isolates were characterized for resistance to a panel of antibiotics, and a subset was subtyped using multilocus sequence typing. Campylobacter was recovered from feces and water for up to 16 days. Analysis of 548 isolates (218 C. jejuni and 330 C. coli) revealed that C. jejuni survived longer than C. coli in feces (P = 0.0005), while the reverse was observed in water (P < 0.0001). Strain-specific differences in survival were noted. Multidrug-resistant C. jejuni isolates of sequence type 1839 (ST-1839) and the related ST-2935 were among the longest-surviving isolates in feces, being recovered for up to 10 to 16 days, while multidrug-resistant C. coli isolates of ST-1101 were recovered from feces for only up to 4 days. Data on Campylobacter survival upon excretion from the birds can contribute to further understanding of the transmission dynamics of this pathogen in the poultry production ecosystem. IMPORTANCE Campylobacter jejuni and Campylobacter coli are leading foodborne pathogens, with poultry as a major reservoir. Due to their growth requirements, these Campylobacter spp. may be unable to replicate once excreted by their avian hosts, but their survival in feces and the environment is critical for transmission in the farm ecosystem. Reducing the prevalence of Campylobacter-positive flocks can have major impacts in controlling both contamination of poultry products and environmental dissemination of the pathogens. However, understanding the capacity of these pathogens to survive in transmission-relevant vehicles such as feces and farmhouse water remains poorly understood, and little information is available on species- and strain-associated differences in survival. Here, we employed model conditions to investigate the survival of C. jejuni and C. coli from naturally colonized turkey flocks, and with diverse genotypes and antimicrobial resistance profiles, in turkey feces and in farmhouse water.

Antibiotic Resistance of Campylobacter jejuni and Campylobacter coli Isolated from Hog, Beef, and Chicken Carcass Samples from Provincially Inspected Abattoirs in Ontario

2006 ◽  
Vol 69 (1) ◽  
pp. 22-26 ◽  
Author(s):  
C. LARKIN ◽  
C. van DONKERSGOED ◽  
A. MAHDI ◽  
P. JOHNSON ◽  
B. McNAB ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
The United States ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Campylobacter Coli ◽  
Resistance Patterns ◽  
Campylobacter Spp

Campylobacter jejuni is one of the most common causes of bacterial foodborne infection in the United States, and there are reports of resistance of Campylobacter spp. to antimicrobial agents used for the treatment of gastroenteritis. The purpose of this study was to determine the antimicrobial resistance patterns of Campylobacter spp. isolated from hog, beef, and chicken carcasses from provincially inspected abattoirs in Ontario. The agar dilution method was performed to measure antimicrobial resistance of the isolates. Antimicrobial resistance of Campylobacter isolates from hogs (n = 401), beef (n = 21), and chicken (n = 435) to ampicillin, azithromycin, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, gentamicin, nalidixic acid, streptomycin, and tetracycline was determined. Resistance of chicken, hog, and beef isolates was 14.3, 18.2, and 9.5% to ampicillin; 17.9, 67.3, and 38.1% to azithromycin; 0, 0.5, and 0% to chloramphenicol; 3.7, 1.2, and 0% to ciprofloxacin; 2.3, 46.6, and 4.8% to clindamycin; 6.7, 43.6, and 4.8% to erythromycin; 0.2, 0, and 0% to gentamicin; 5.1, 10.7, and 0% to nalidixic acid; 13.6, 57.4, and 4.8% to streptomycin; and 52.6, 44.1, 42.9% to tetracycline, respectively. The hog isolates had the greatest resistance to seven of the ten antimicrobials tested. Results of this study confirm the existence of antimicrobial resistance of Campylobacter to various antimicrobial agents, especially ciprofloxacin and erythromycin, commonly used for treatment of campylobacteriosis in humans.

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