scholarly journals Occurrence of 1-Glyceryl-1-myo-Inosityl Phosphate in Hyperthermophiles

2006 ◽  
Vol 72 (9) ◽  
pp. 6169-6173 ◽  
Author(s):  
Pedro Lamosa ◽  
Lu�s G. Gon�alves ◽  
Marta V. Rodrigues ◽  
L�gia O. Martins ◽  
Neil D. H. Raven ◽  
...  
Keyword(s):  
Inositol Phosphate ◽  
Compatible Solutes ◽  
Archaeoglobus Fulgidus ◽  
Nuclear Magnetic Resonance Analysis ◽  
Unknown Compound ◽  
Intracellular Pool ◽  
Resonance Analysis ◽  
Cell Extracts ◽  
Hyperthermophilic Bacterium ◽  
Nacl Concentration

ABSTRACT The accumulation of compatible solutes was studied in the hyperthermophilic bacterium Aquifex pyrophilus as a function of the temperature and the NaCl concentration of the growth medium. Nuclear magnetic resonance analysis of cell extracts revealed the presence of α- and β-glutamate, di-mannosyl-di-myo-inositol phosphate, di-myo-inositol phosphate, and an additional compound here identified as 1-glyceryl-1-myo-inosityl phosphate. All solutes accumulated by A. pyrophilus are negatively charged at physiological pH. The intracellular levels of di-myo-inositol phosphate increased in response to supraoptimal growth temperature, while α- and β-glutamate accumulated in response to osmotic stress, especially at growth temperatures below the optimum. The newly discovered compound, 1-glyceryl-1-myo-inosityl phosphate, appears to play a double role in osmo- and thermoprotection, since its intracellular pool increased primarily in response to a combination of osmotic and heat stresses. This work also uncovered the nature of the unknown compound, previously detected in Archaeoglobus fulgidus (L. O. Martins et al., Appl. Environ. Microbiol. 63:896-902, 1997). The curious structural relationship between diglycerol phosphate (found only in Archaeoglobus species), di-myo-inositol phosphate (a canonical solute of hyperthermophiles), and the newly identified solute is highlighted. This is the first report on the occurrence of 1-glyceryl-1-myo-inosityl phosphate in living systems.

scholarly journals Biosynthetic Pathways of Inositol and Glycerol Phosphodiesters Used by the Hyperthermophile Archaeoglobus fulgidus in Stress Adaptation

2006 ◽  
Vol 188 (23) ◽  
pp. 8128-8135 ◽  
Author(s):  
Nuno Borges ◽  
Luís G. Gonçalves ◽  
Marta V. Rodrigues ◽  
Filipa Siopa ◽  
Rita Ventura ◽  
...  
Keyword(s):  
Inositol Phosphate ◽  
Compatible Solutes ◽  
Regulatory Mechanisms ◽  
Archaeoglobus Fulgidus ◽  
Nuclear Magnetic Resonance Analysis ◽  
Biosynthetic Pathways ◽  
Inositol 1 ◽  
Resonance Analysis ◽  
Intermediate Metabolites

ABSTRACT Archaeoglobus fulgidus accumulates di-myo-inositol phosphate (DIP) and diglycerol phosphate (DGP) in response to heat and osmotic stresses, respectively, and the level of glycero-phospho-myo-inositol (GPI) increases primarily when the two stresses are combined. In this work, the pathways for the biosynthesis of these three compatible solutes were established based on the detection of the relevant enzymatic activities and characterization of the intermediate metabolites by nuclear magnetic resonance analysis. The synthesis of DIP proceeds from glucose-6-phosphate via four steps: (i) glucose-6-phosphate was converted into l-myo-inositol 1-phosphate by l-myo-inositol 1-phosphate synthase; (ii) l-myo-inositol 1-phosphate was activated to CDP-inositol at the expense of CTP; this is the first demonstration of CDP-inositol synthesis in a biological system; (iii) CDP-inositol was coupled with l-myo-inositol 1-phosphate to yield a phosphorylated intermediate, 1,1′-di-myo-inosityl phosphate 3-phosphate (DIPP); (iv) finally, DIPP was dephosphorylated into DIP by the action of a phosphatase. The synthesis of the two other polyol-phosphodiesters, DGP and GPI, proceeds via the condensation of CDP-glycerol with the respective phosphorylated polyol, glycerol 3-phosphate for DGP and l-myo-inositol 1-phosphate for GPI, yielding the respective phosphorylated intermediates, 1X,1′X-diglyceryl phosphate 3-phosphate (DGPP) and 1-(1X-glyceryl) myo-inosityl phosphate 3-phosphate (GPIP), which are subsequently dephosphorylated to form the final products. The results disclosed here represent an important step toward the elucidation of the regulatory mechanisms underlying the differential accumulation of these compounds in response to heat and osmotic stresses.

scholarly journals Tunneling Spectroscopy by Nuclear Magnetic Resonance: Analysis of Rotational Tunneling in Solid Pentamethylbenzene

1985 ◽  
Vol 40 (11) ◽  
pp. 1075-1084
Author(s):  
W. T. Sobol ◽  
K.R. Sridharan ◽  
I. G. Cameron ◽  
M. M. Pintar
Keyword(s):  
Frequency Dependence ◽  
Lattice Relaxation ◽  
Time Correlation ◽  
Lattice Relaxation Time ◽  
Polarization Transfer ◽  
Spin Lattice Relaxation ◽  
Transfer Model ◽  
Nuclear Magnetic Resonance Analysis ◽  
Spin Lattice ◽  
Resonance Analysis

The frequency dependence of the spin-lattice relaxation time T1 was measured at three temperatures near one of the Zeeman-tunneling level matching resonances for pentamethylbenzene. These measurements are correlated with 71 temperature dependence data from the literature. It is shown that the frequency dependence of the Zeeman-torsion coupling time cannot be explained in terms of the semiclassical perturbation theory using time correlation functions. A three bath polarization transfer model is also employed and the applicability of both models discussed. Zeeman-torsion coupling is further investigated using a saturation sequence and the results are compared with the predictions of the three bath polarization transfer model.

Polymyrcene microstructure revisited from precise high-field nuclear magnetic resonance analysis

Polymer ◽  
2014 ◽  
Vol 55 (16) ◽  
pp. 3869-3878 ◽  
Author(s):  
Sébastien Georges ◽  
Marc Bria ◽  
Philippe Zinck ◽  
Marc Visseaux
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Field ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
Nuclear Magnetic

scholarly journals New compatible solutes related to Di-myo-inositol-phosphate in members of the order Thermotogales.

1996 ◽  
Vol 178 (19) ◽  
pp. 5644-5651 ◽  
Author(s):  
L O Martins ◽  
L S Carreto ◽  
M S Da Costa ◽  
H Santos
Keyword(s):  
Inositol Phosphate ◽  
Compatible Solutes
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