Investigating the Molecular Basis for the Virulence of Escherichia coli K5 by Nuclear Magnetic Resonance Analysis of the Capsule Polysaccharide

2009 ◽  
Vol 17 (2) ◽  
pp. 71-82 ◽  
Author(s):  
Charles D. Blundell ◽  
Ian S. Roberts ◽  
John K. Sheehan ◽  
Andrew Almond
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Molecular Basis ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
Nuclear Magnetic

scholarly journals Carbon-13 Nuclear Magnetic Resonance Study of Metabolism of Propionate by Escherichia coli

1999 ◽  
Vol 181 (11) ◽  
pp. 3562-3570 ◽  
Author(s):  
Robert E. London ◽  
Devon L. Allen ◽  
Scott A. Gabel ◽  
Eugene F. DeRose
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Tca Cycle ◽  
Nuclear Magnetic Resonance Study ◽  
Nuclear Magnetic Resonance Analysis ◽  
Pyruvate Formate Lyase ◽  
Resonance Analysis ◽  
Direct Formation ◽  
Nuclear Magnetic

ABSTRACT We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the13C-13C-12C carbon skeleton in labeled alanine and alanine-containing peptides involved in cell wall recycling is indicative of the direct formation of pyruvate from propionate via the methylcitrate cycle, the enzymes of which have recently been demonstrated in E. coli. Additionally, formation of 13C-labeled formate from pyruvate by the action of pyruvate-formate lyase is also consistent with the labeling of pyruvate C-1. Carboxylation of the labeled pyruvate leads to formation of [1,2-13C2]oxaloacetate and to multiply labeled glutamate and succinate isotopomers, also consistent with the flux through the methylcitrate pathway, followed by the tricarboxylic acid (TCA) cycle. Additional labeling of TCA intermediates arises due to the formation of [1-13C]acetyl coenzyme A from the labeled pyruvate, formed via pyruvate-formate lyase. Labeling patterns in trehalose and glycine are also interpreted in terms of the above pathways. The information derived from the [1,2-13C2]propionate label is contrasted with information which can be derived from singly or triply labeled propionate and shown to be more useful for distinguishing the different propionate utilization pathways via nuclear magnetic resonance analysis.

Polymyrcene microstructure revisited from precise high-field nuclear magnetic resonance analysis

Polymer ◽  
2014 ◽  
Vol 55 (16) ◽  
pp. 3869-3878 ◽  
Author(s):  
Sébastien Georges ◽  
Marc Bria ◽  
Philippe Zinck ◽  
Marc Visseaux
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Field ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
Nuclear Magnetic
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