Amping up HIV antibodies

Science ◽  
2021 ◽  
Vol 372 (6549) ◽  
pp. 1397-1398
Author(s):  
Dennis R. Burton
Keyword(s):  
Hiv Antibodies

AIDS in a Hemophiliac Child Transmitted by Cryoprecipitate and with Negative HIV Antibodies by Western Blot

1990 ◽  
Vol 4 (2) ◽  
pp. 141-146
Author(s):  
JULIE M. SLIGH ◽  
ALAN P. KNUTSEN ◽  
JOHN D. BOUHASIN
Keyword(s):  
Western Blot ◽  
Hiv Antibodies

Plasmablastic Myeloma Versus Plasmablastic Lymphoma; Is MYC Rearrangement Helpful Towards A Precise Diagnosis?

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S105-S105
Author(s):  
M Khedr ◽  
Y Yusuf ◽  
L Aftab
Keyword(s):  
Reference Range ◽  
Final Diagnosis ◽  
Plasmablastic Lymphoma ◽  
Bone Lesions ◽  
Molecular Testing ◽  
Diagnostic Challenge ◽  
Plasma Cell Neoplasm ◽  
Hiv Antibodies ◽  
Precise Diagnosis ◽  
Diagnostic Work

Abstract Introduction/Objective Plasmablastic myeloma (PBM) is a rare and aggressive plasma cell neoplasm. Differentiating PBM from plasmablastic lymphoma (PBL) represents a diagnostic challenge, as both diseases have overlapping cytomorphologic and immunophenotypic features. Genetic mutations in MYC occur in a majority of PBL cases but rarely in PBM, thus can theoretically be used to differentiate between both neoplasms. Methods We report a case of a 53-year-old female who presented with a rapidly growing mass in her right mandible. Biopsy revealed circumscribed nodules of immunoblastic cells with moderate cytoplasm, large vesicular nuclei and large prominent nucleoli. Apoptotic debris and brisk mitoses were present. Molecular testing revealed a C-MYC rearrangement. The location of the neoplasm and the above described morphological features were suggestive of PBL, especially with a positive C-MYC rearrangement. The neoplastic cells were positive for CD138, MUM1,CD56 and kappa; and negative for CD45, CD20, PAX5, CD3, CD5, CD30, EBER-ISH, HHV8, ALK-1, Lambda, EMA, CD21, CD23, pancytokeratin, CK20, CK7, Cam5.2, chromogranin, synaptophysin, HMB45, S100, P16, P40. MIB-1 showed high positivity, approximately 95%. Results Patient underwent further diagnostic work up, her HIV antibodies result were negative however, she was found to be anemic (Hemoglobin 6.6 g/dl; reference range 12-16 g/dl) and hypercalcemic (Calcium 12.3 mg/dl; reference range 8.5-10.5 mg/dl). PET scan revealed multiple hypermetabolic lytic bone lesions. The bone marrow biopsy showed 80% cellularity with extensive involvement by atypical plasmacytic cells forming large clusters. The patient’s final diagnosis was PBM. Conclusion Differentiating PBM from PBL is essential as treatment is different. Although MYC rearrangement in PBM is not common, it has been demonstrated and therefore should not be used to exclude this diagnosis. Here, we highlight the importance of correlating detailed clinical, radiological, laboratory, histological and genetics data for reaching the final diagnosis.

scholarly journals How long is long-term? Delivery of anti-HIV antibodies using AAV vector

2019 ◽  
Vol 5 ◽  
pp. 49
Author(s):  
J. Martinez-Navio ◽  
R. Desrosiers ◽  
S. Fuchs ◽  
D. Mendes ◽  
E. Rakasz ◽  
...  
Keyword(s):  
Aav Vector ◽  
Term Delivery ◽  
Hiv Antibodies ◽  
Anti Hiv

scholarly journals Validation study of a conventional enzyme immunoassay to detect HIV antibodies in oral fluid

2016 ◽  
Vol 116 (01) ◽  
pp. 19-21
Author(s):  
D. Stanekova ◽  
M. Mirandola ◽  
L. Gios ◽  
C. Botsi ◽  
M. Habekova ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Validation Study ◽  
Oral Fluid ◽  
Hiv Antibodies

Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots –a feasibility study using a multi-analyte immunoassay

2005 ◽  
Vol 43 (2) ◽  
Author(s):  
Zoltan Lukacs ◽  
Alexandra Dietrich ◽  
Rainer Ganschow ◽  
Alfried Kohlschütter ◽  
Rudolf Kruithof
Keyword(s):  
Hepatitis C ◽  
Hepatitis B ◽  
Simultaneous Detection ◽  
Dried Blood Spots ◽  
Hiv Positive ◽  
Blood Spots ◽  
Hiv Positive Mothers ◽  
Hiv Antibodies ◽  
Hepatitis B Antigens ◽  
Antibody Levels

AbstractHIV in particular, as well as hepatitis B and C, present a burden on healthcare systems worldwide. Early detection of these diseases may prevent further infections and improve the outcome for patients. In particular, transmission of HIV from mother to child can be significantly reduced when preventive measures are taken before birth. We have developed and optimized a method for the simultaneous detection of HIV 1 and hepatitis B and C from dried blood specimensusing the Luminex multi-analyte profiling technology (LabMap). Dried blood spots provide a convenient method for mailing, analysis and storage of samples. Specimens from known HIV-positive children (n=46) as well as hepatitis B- (n=8) and hepatitis C-positive patients (n=7) tested positive in our assay. Storage for up to 10years did not interfere with the test in the case of HIV-positive patients. Results for five different antibodies and one antigen were obtained in approximately 80seconds. Furthermore, antibody levels in infants of HIV-positive mothers were monitored over a period of 1year. Antibodies were no longer detectable after 260–360days, which compared well with results independently obtained by ELISA and Western blot analysis. We demonstrated the feasibility of the simultaneous detection of infectious diseases from dried blood. Our novel method also provides a convenient tool for monitoring children from HIV-positive mothers and for possible screening efforts.

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