scholarly journals Validation study of a conventional enzyme immunoassay to detect HIV antibodies in oral fluid

2016 ◽  
Vol 116 (01) ◽  
pp. 19-21
Author(s):  
D. Stanekova ◽  
M. Mirandola ◽  
L. Gios ◽  
C. Botsi ◽  
M. Habekova ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Validation Study ◽  
Oral Fluid ◽  
Hiv Antibodies

42. PILOT OF NON-INVASIVE (ORAL FLUID) TESTING FOR HIV WITHIN A COMMUNITY SETTING

Sexual Health ◽  
2007 ◽  
Vol 4 (4) ◽  
pp. 300
Author(s):  
S. Staunton ◽  
J. Debattista ◽  
N. Roudenko ◽  
C. Davis
Keyword(s):  
Oral Fluid ◽  
Community Setting ◽  
Risk Behaviour ◽  
Community Response ◽  
Numerical Code ◽  
Sexual Risk Behaviour ◽  
Hiv Status ◽  
Hiv Surveillance ◽  
Hiv Antibodies ◽  
Gay Bars

An anonymous HIV surveillance study was conducted to determine the prevalence of HIV amongst patrons attending gay recreational venues, the level of undiagnosed HIV infection and to identify sexual risk behaviour associated with HIV positive, HIV negative and unknown serostatus. 427 men who have sex with men were recruited over a period of one week in various sex on premises venues and gay bars within the inner city of Brisbane. Oral fluid testing for HIV antibodies was undertaken using the Orasure collection system and assay. Each participant was invited to complete a brief behaviour questionnaire and submit an oral fluid specimen. Participants were also asked their HIV status. Surveys and specimens were linked using an anonymous numerical code. Surveys were analysed using Epi-Info. Oral swabs were tested for the presence of HIV antibodies and any reactive specimens were confirmed using an Orasure western Blot. Confirmed serology results were linked to reported sexual behaviours, testing patterns and HIV status. The results of this study - sexual and testing behaviour correlated with serostatus- and implications for HIV prevention programs will be presented. As well as that, discussions will be held regarding the community response to the project.

scholarly journals Efficacy of a Less-Sensitive Enzyme Immunoassay (3A11-LS) for Early Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Infants

2001 ◽  
Vol 8 (6) ◽  
pp. 1282-1285
Author(s):  
Debra Candal ◽  
Marc Bulterys ◽  
Elaine J. Abrams ◽  
Richard W. Steketee ◽  
Bharat S. Parekh
Keyword(s):  
Human Immunodeficiency Virus ◽  
Early Diagnosis ◽  
Enzyme Immunoassay ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunodeficiency Virus ◽  
Hiv Antibodies ◽  
Test Result ◽  
Rule Out

ABSTRACT We evaluated a less-sensitive enzyme immunoassay (3A11-LS) for its possible use for early diagnosis of human immunodeficiency virus type 1 (HIV-1) infection in infants. The results were compared with those from the immunoglobulin G-capture enzyme immunoassay. A total of 239 sera from 77 infants were tested. All 25 sera from the 10 infants born to seronegative mothers were found to be negative by both assays. Forty-one seroreverting infants showed a complete decay of maternal antibodies by 4 months by the 3A11-LS assay. However, the assay detected HIV antibodies in only 9 (36%) of 25 sera collected from infected infants between 4 and 6 months and in 27 (63%) of 43 sera collected after 6 months of age. Further analysis with alternative cutoff values indicated that the 3A11-LS had a sensitivity of 12 to 44% and a specificity of 90 to 100% for infants between 4–6 months of age. This data suggest that a diagnosis of HIV infection in some of the infants could be made after 4 months of age by the 3A11-LS assay, although a negative 3A11-LS test result may not rule out infection and may require a further followup.

Pilot of non-invasive (oral fluid) testing for HIV within a community setting

Sexual Health ◽  
2010 ◽  
Vol 7 (1) ◽  
pp. 11 ◽  
Author(s):  
Frances Birrell ◽  
Shaun Staunton ◽  
Joseph Debattista ◽  
Nicole Roudenko ◽  
William Rutkin ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Community Setting ◽  
Risk Behaviour ◽  
Hiv Positive ◽  
Hiv Status ◽  
Significant Difference ◽  
Hiv Antibodies ◽  
Undiagnosed Hiv ◽  
Gay Bars ◽  
Sex With Men

Background: The present study sought to determine the level of undiagnosed HIV infection within a community setting of men who have sex with men (MSM) and identify any associated sexual risk behaviours. Methods: A total of 427 MSM were recruited in sex-on-premises venues (SOPV) and gay bars within the inner city of Brisbane. An additional 37 MSM were recruited in a smaller, regional centre (Toowoomba). Oral fluid testing for HIV antibodies was undertaken using the Orasure collection system and assay. Each participant was invited to complete a brief behaviour questionnaire and submit an oral fluid specimen. Confirmed serology results were linked to reported sexual behaviours, testing patterns and HIV status. Results: Of the 464 men surveyed, 33 identified as HIV-positive, and all of these were reactive by the Orasure assay. A further eight people who identified as negative or unknown serostatus, had confirmed Orasure reactive results, resulting in 1.9% of the ‘non-HIV positive’ MSM sample unaware of their positive HIV status. Therefore, 19.5% of the total confirmed HIV-positive individuals were not aware of their true serostatus. Conclusions: A significant minority of HIV-positive MSM are currently unaware of their positive serostatus. However, an analysis of their risk behaviour does not seem to indicate any significant difference to those who are HIV-negative. Interestingly, 86% of those who were unaware they were HIV-positive identified that they had been tested in the previous 6 months and all of them claimed to have been tested in the previous 2 years.

Reaction specificity and sensitivity of screening enzyme immunoassay kits to detect anti-HIV antibodies.

1989 ◽  
Vol 35 (5) ◽  
pp. 514-517
Author(s):  
Hisahiro Yoshida ◽  
Kimiko Yurugi ◽  
Kazuhiko Ito ◽  
Hiroo Saji ◽  
Etsuko Maruya
Keyword(s):  
Enzyme Immunoassay ◽  
Specificity And Sensitivity ◽  
Reaction Specificity ◽  
Hiv Antibodies ◽  
Anti Hiv

Adult and paediatric AIDS patients lacking HIV antibodies detected by enzyme immunoassay in central Africa

AIDS ◽  
1989 ◽  
Vol 3 (7) ◽  
pp. 429-432 ◽  
Author(s):  
Philippe Van de Perre ◽  
Philippe Lepage ◽  
Jean Batungwanayo ◽  
Daniel Bonnet
Keyword(s):  
Enzyme Immunoassay ◽  
Central Africa ◽  
Aids Patients ◽  
Hiv Antibodies

Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid

1996 ◽  
Vol 15 (11) ◽  
pp. 882-884 ◽  
Author(s):  
P. G. McIntyre ◽  
J. Laszlo ◽  
K. Appleyard ◽  
G. R. Ogden
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Enzyme Immunoassay ◽  
Oral Fluid ◽  
Modified Enzyme

scholarly journals Antibody detection by agglutination–PCR (ADAP) enables early diagnosis of HIV infection by oral fluid analysis

2018 ◽  
Vol 115 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
Cheng-ting Tsai ◽  
Peter V. Robinson ◽  
Felipe de Jesus Cortez ◽  
Maria L. B. Elma ◽  
David Seftel ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Detection Threshold ◽  
Population Based ◽  
Antibody Detection ◽  
Analytical Sensitivity ◽  
Sample Collection ◽  
Clinical Sensitivity ◽  
Fluid Analysis ◽  
Hiv Antibodies ◽  
Anti Hiv

Oral fluid (OF) is a highly effective substrate for population-based HIV screening efforts, as it is noninfectious and significantly easier to collect than blood. However, anti-HIV antibodies are found at far lower concentrations in OF compared with blood, leading to poor sensitivity and a longer period of time from infection to detection threshold. Thus, despite its inherent advantages in sample collection, OF is not widely used for population screening. Here we report the development of an HIV OF assay based on Antibody Detection by Agglutination–PCR (ADAP) technology. This assay is 1,000–10,000 times more analytically sensitive than clinical enzyme-linked immunoassays (EIAs), displaying both 100% clinical sensitivity and 100% specificity for detecting HIV antibodies within OF samples. We show that the enhanced analytical sensitivity enables this assay to correctly identify HIV-infected individuals otherwise missed by current OF assays. We envision that the attributes of this improved HIV OF assay can increase testing rates of at-risk individuals while enabling diagnosis and treatment at an earlier time point.

Detecting HIV antibodies in oral fluid: validation of a commercial antigen-antibody assay

2013 ◽  
Vol 70 (3) ◽  
pp. 125-128
Author(s):  
S. Mortlock ◽  
F. Mclean ◽  
C. Pickford ◽  
S. Willis
Keyword(s):  
Oral Fluid ◽  
Antibody Assay ◽  
Hiv Antibodies ◽  
Antigen Antibody

Pilot validation study of a rapid test in oral fluid for population screening of hepatitis C virus infection

2017 ◽  
Vol 66 (1) ◽  
pp. S403-S404 ◽  
Author(s):  
C. Pallarés ◽  
A.S. Carvalho-Gomes ◽  
I. Conde ◽  
T. Di Maira ◽  
V. Hontangas ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Validation Study ◽  
Oral Fluid ◽  
Rapid Test ◽  
Population Screening ◽  
Hepatitis C Virus Infection
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