scholarly journals Reticulocyte transfer RNA and hemoglobin synthesis

Science ◽  
1976 ◽  
Vol 193 (4251) ◽  
pp. 428-429 ◽  
Author(s):  
M Litt
Keyword(s):  
Transfer Rna ◽  
Hemoglobin Synthesis

Reticulocyte transfer RNA and hemoglobin synthesis

Science ◽  
1975 ◽  
Vol 190 (4214) ◽  
pp. 529-535 ◽  
Author(s):  
D. Smith
Keyword(s):  
Transfer Rna ◽  
Hemoglobin Synthesis

Regulation of Hemoglobin Synthesis in the Blood Islands of Chick Blastodiscs. Tentative Identification of the Stimulatory Transfer RNA as a Minor Alanine-Specific Species

1972 ◽  
Vol 50 (11) ◽  
pp. 1158-1164 ◽  
Author(s):  
S. D. Wainwright ◽  
Lillian K. Wainwright ◽  
H. M. Tsay
Keyword(s):  
Actinomycin D ◽  
Deae Cellulose ◽  
Transfer Rna ◽  
High Concentration ◽  
Adult Chicken ◽  
Tentative Identification ◽  
Hemoglobin Synthesis ◽  
Amino Acid Mixtures ◽  
Specific Species ◽  
A Minor

Transfer RNA, isolated from adult chicken liver and from the membranes associated with the 5- to 6-day embryo, stimulated hemoglobin formation by de-embryonated chick blastodiscs explanted at the 5-somite stage onto medium containing a high concentration of actinomycin D. Preparations acylated specifically with alanine retained this biological activity when oxidized with periodate. The activity of preparations charged with amino acid mixtures lacking alanine was destroyed by periodate treatment.A minor species, representing less than 1% of the total, was largely resolved from the bulk of the alanine-specific tRNAs of chick liver by serial chromatography on benzoylated DEAE-cellulose. The isolated fraction contained the component which stimulated hemoglobin formation by blastodiscs explanted onto medium containing a high concentration of actinomycin.

Comparison of the Transfer RNA Complements of Chick Embryos and their Supporting Membranes

1972 ◽  
Vol 50 (10) ◽  
pp. 1056-1063 ◽  
Author(s):  
S. D. Wainwright ◽  
Julia C. Thompson ◽  
J. F. Prchal ◽  
H. M. Tsay
Keyword(s):  
Minor Component ◽  
Transfer Rna ◽  
Quantitative Change ◽  
Chick Embryos ◽  
Hemoglobin Synthesis ◽  
Distribution Of Components

The transfer RNA complement of 5- to 6-day chick embryos was compared with that of the associated membranes by chromatography of preparations of aminoacyl-tRNAs on columns of benzoylated diethyl-aminoethylcellulose. Profiles of all aminoacyl-tRNAs were determined under conditions selected for emphasis on the region containing a component believed to play a role in regulating onset of rapid hemoglobin synthesis in the blood islands of young blastodiscs. Differences in profile were observed only for alanyl- and glutamyl-tRNAs. A marked quantitative change in the distribution of components in the complement of glutamyl-tRNAs was in the converse direction of that required for increase in content of putative regulator and was not further investigated.Small reproducible differences in the profiles of alanyl-tRNAs were further investigated and were shown to be due to a marked increase in the content of a distinct minor component of the alanyl-tRNA complement of the membranes relative to that of the embryo.

Response : Reticulocyte Transfer RNA and Hemoglobin Synthesis

Science ◽  
1976 ◽  
Vol 193 (4251) ◽  
pp. 429-429
Author(s):  
David W. E. Smith
Keyword(s):  
Transfer Rna ◽  
Hemoglobin Synthesis

Specialization of Rabbit Reticulocyte Transfer RNA Content for Hemoglobin Synthesis

Science ◽  
1971 ◽  
Vol 171 (3971) ◽  
pp. 577-579 ◽  
Author(s):  
D. W. E. Smith ◽  
A. L. McNamara
Keyword(s):  
Transfer Rna ◽  
Hemoglobin Synthesis ◽  
Rna Content

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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