Genetic rescue of a lethal "null" activity allele of 6-phosphogluconate dehydrogenase in Drosophila melanogaster

Science ◽  
1977 ◽  
Vol 196 (4294) ◽  
pp. 1114-1115 ◽  
Author(s):  
M. Hughes ◽  
J. Lucchesi
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Rescue ◽  
Phosphogluconate Dehydrogenase

scholarly journals A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene

2001 ◽  
Vol 78 (1) ◽  
pp. 13-21 ◽  
Author(s):  
H. GRANOK ◽  
B. A. LEIBOVITCH ◽  
S. C. R. ELGIN
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Position Effect ◽  
Efficient Production ◽  
Position Effect Variegation ◽  
Chromosomal Protein ◽  
Gaga Factor ◽  
Genetic Rescue ◽  
Regulation Of Synthesis

GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of GAGA factor, or for more precise regulation of synthesis, to carry out the diverse functions of this protein.

Interspecific hybridization between Drosophila species group melanogaster sibling species: isozymic patterns and reproductive relationships

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 146-154 ◽  
Author(s):  
G. N. Goulielmos ◽  
S. N. Alahiotis
Keyword(s):  
Drosophila Melanogaster ◽  
Sex Ratio ◽  
Interspecific Hybrids ◽  
Aldehyde Oxidase ◽  
Species Group ◽  
Fitness Components ◽  
Phosphogluconate Dehydrogenase ◽  
Evolutionary Changes ◽  
Fertile Hybrids ◽  
Male Genital

In spite of previous consensus that no F1 fertile hybrids (of both sexes) could be produced between any mating combination of Drosophila melanogaster, D. simulans, and D. mauritiana, the present data indicate that such hybrids were obtained. Thus, some crosses between D. mauritiana females and D. simulans or D. melanogaster males yield F1 fertile hybrids (of both sexes) which have been named Masi (or Masi-2 and Masi-3) and Mame, respectively. Electrophoretic studies, using the species-diagnostic genes for 6-phosphogluconate dehydrogenase, alcohol dehydrogenase, and aldehyde oxidase (6-Pgd, Adh, and Aldox, respectively), were used to investigate the hybrid status, taking into consideration (i) their reproductive relationships, (ii) the coexistence of electromorphs from different species in the same hybrid, within the same generation, and (iii) the expression of the above electromorphs in the hybrids as well as in progeny from backcrosses, where unexpected irregularities and abnormalities were observed. These interspecific hybrids have been kept in our laboratory (as stocks) for 50 generations, to date, and have also been tested for various characteristics that contributed to the verification of their hybrid status (mating abilities, enzyme activities, hybrid sex ratio, the morphology of male genital arches and other fitness components). The finding of major genetic phenomena (e.g., allozymic repression) in these hybrid genomes gives some idea of the nature of events that could be associated with strong evolutionary changes, thus controlling speciation processes.Key words: Drosophila, electrophoresis, electromorphs, interspecific hybrids.

scholarly journals LETHAL EFFECTS OF LOW AND "NULL" ACTIVITY ALLELES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN DROSOPHILA MELANOGASTER

Genetics ◽  
1975 ◽  
Vol 79 (3) ◽  
pp. 451-457
Author(s):  
Glenn C Bewley ◽  
John C Lucchesi
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Enzymatic Activity ◽  
Null Allele ◽  
Developmental Rate ◽  
Lethal Effects ◽  
Homozygous Condition ◽  
Phosphogluconate Dehydrogenase ◽  
Relative Viability ◽  
Low Activity

ABSTRACT EMS-induced "null" and low activity alleles for 6-phosphogluconate dehydrogenase were characterized with respect to enzymatic activity, relative viability, fertility, and the effective lethal phase. It was determined that flies hemizygous and homozygous for the low activity allele, Pgd  -, possessed a depressed developmental rate, diminished viability, and loss of female fertility. Heterozygotes for Pgd  - and a deficiency for Pgd  + were lethal. The "null" activity allele demonstrated a lethal phenotype in both the hemizygous and homozygous condition. The effective lethal phase for the "null" allele occurs during late embryonic development (20-22 hr).

MATERNALLY INFLUENCED EMBRYONIC LETHALITY: ALLELE SPECIFIC GENETIC RESCUE AT A FEMALE FERTILITY LOCUS IN DROSOPHILA MELANOGASTER

1976 ◽  
Vol 18 (4) ◽  
pp. 773-781 ◽  
Author(s):  
Patricia Romans ◽  
R. B. Hodgetts ◽  
D. Nash
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
X Chromosome ◽  
Maternal Effect ◽  
Female Offspring ◽  
The Other ◽  
Genetic Rescue ◽  
Homozygous Mutant ◽  
Allele Specific ◽  
Normal Males

A new locus, mel(l)R1, with a maternal effect on embryonic development, has been mapped at about 0.5 on the X chromosome of Drosophila melanogaster and localized cytologically between bands 2D6 and 3A1. Genotypically mutant embryos die if produced by homozygous mutant females but survive if produced by heterozygous females. Two mutant alleles have been isolated. One of these is genetically rescuable: when homozygous mutant females are mated to mutant males, all the embryos die, but when these females are mated to normal males, female offspring are produced. The other allele is not rescuable. Genetic rescue is dominant at this locus since females heterozygous for the two mutant alleles produce female offspring in crosses to normal males.

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