Dietary rescue of a lethal ?null? activity allele of 6-phosphogluconate dehydrogenase in Drosophila melanogaster

1978 ◽  
Vol 16 (5-6) ◽  
pp. 469-475 ◽  
Author(s):  
M. Beatrice Hughes ◽  
John C. Lucchesi
Keyword(s):  
Drosophila Melanogaster ◽  
Phosphogluconate Dehydrogenase

Interspecific hybridization between Drosophila species group melanogaster sibling species: isozymic patterns and reproductive relationships

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 146-154 ◽  
Author(s):  
G. N. Goulielmos ◽  
S. N. Alahiotis
Keyword(s):  
Drosophila Melanogaster ◽  
Sex Ratio ◽  
Interspecific Hybrids ◽  
Aldehyde Oxidase ◽  
Species Group ◽  
Fitness Components ◽  
Phosphogluconate Dehydrogenase ◽  
Evolutionary Changes ◽  
Fertile Hybrids ◽  
Male Genital

In spite of previous consensus that no F1 fertile hybrids (of both sexes) could be produced between any mating combination of Drosophila melanogaster, D. simulans, and D. mauritiana, the present data indicate that such hybrids were obtained. Thus, some crosses between D. mauritiana females and D. simulans or D. melanogaster males yield F1 fertile hybrids (of both sexes) which have been named Masi (or Masi-2 and Masi-3) and Mame, respectively. Electrophoretic studies, using the species-diagnostic genes for 6-phosphogluconate dehydrogenase, alcohol dehydrogenase, and aldehyde oxidase (6-Pgd, Adh, and Aldox, respectively), were used to investigate the hybrid status, taking into consideration (i) their reproductive relationships, (ii) the coexistence of electromorphs from different species in the same hybrid, within the same generation, and (iii) the expression of the above electromorphs in the hybrids as well as in progeny from backcrosses, where unexpected irregularities and abnormalities were observed. These interspecific hybrids have been kept in our laboratory (as stocks) for 50 generations, to date, and have also been tested for various characteristics that contributed to the verification of their hybrid status (mating abilities, enzyme activities, hybrid sex ratio, the morphology of male genital arches and other fitness components). The finding of major genetic phenomena (e.g., allozymic repression) in these hybrid genomes gives some idea of the nature of events that could be associated with strong evolutionary changes, thus controlling speciation processes.Key words: Drosophila, electrophoresis, electromorphs, interspecific hybrids.

scholarly journals LETHAL EFFECTS OF LOW AND "NULL" ACTIVITY ALLELES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN DROSOPHILA MELANOGASTER

Genetics ◽  
1975 ◽  
Vol 79 (3) ◽  
pp. 451-457
Author(s):  
Glenn C Bewley ◽  
John C Lucchesi
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Enzymatic Activity ◽  
Null Allele ◽  
Developmental Rate ◽  
Lethal Effects ◽  
Homozygous Condition ◽  
Phosphogluconate Dehydrogenase ◽  
Relative Viability ◽  
Low Activity

ABSTRACT EMS-induced "null" and low activity alleles for 6-phosphogluconate dehydrogenase were characterized with respect to enzymatic activity, relative viability, fertility, and the effective lethal phase. It was determined that flies hemizygous and homozygous for the low activity allele, Pgd  -, possessed a depressed developmental rate, diminished viability, and loss of female fertility. Heterozygotes for Pgd  - and a deficiency for Pgd  + were lethal. The "null" activity allele demonstrated a lethal phenotype in both the hemizygous and homozygous condition. The effective lethal phase for the "null" allele occurs during late embryonic development (20-22 hr).

scholarly journals REGULATION OF GENE FUNCTION: A COMPARISON OF X-LINKED ENZYME ACTIVITY LEVELS IN NORMAL AND INTERSEXUAL TRIPLOIDS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1973 ◽  
Vol 73 (3) ◽  
pp. 459-464
Author(s):  
John C Lucchesi ◽  
John M Rawls
Keyword(s):  
Enzyme Activity ◽  
Drosophila Melanogaster ◽  
Gene Function ◽  
Normal Male ◽  
Activity Levels ◽  
Linked Gene ◽  
Phosphogluconate Dehydrogenase ◽  
Diploid Female ◽  
Level Of Activity ◽  
Dehydrogenase Enzyme

ABSTRACT We have measured gene function in normal and male-like intersexual triploids of Drosophila melanogaster by assaying crude extracts of whole flies or thoraces for levels of an X-linked (6-phosphogluconate dehydrogenase) and an autosomal (NADP-dependent isocitrate dehydrogenase) enzyme activity. Our observations lead us to conclude that each dose of the X-linked gene is more active in the cells of these intersexes than it is in normal triploid or diploid female cells. These results indicate that a level of activity intermediate between the normal male and female levels is possible for X-linked genes.

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