Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells

Science ◽  
1988 ◽  
Vol 242 (4877) ◽  
pp. 426-430 ◽  
Author(s):  
S Nakamura ◽  
S. Salahuddin ◽  
P Biberfeld ◽  
B Ensoli ◽  
P. Markham ◽  
...  
Keyword(s):  
T Cells ◽  
Growth Factor ◽  
Cd4 T Cells ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Long Term Culture ◽  
Sarcoma Cells

Identification of a major growth factor for AIDS-Kaposi's sarcoma cells as oncostatin M

Science ◽  
1992 ◽  
Vol 255 (5050) ◽  
pp. 1430-1432 ◽  
Author(s):  
B. Nair ◽  
A. DeVico ◽  
S Nakamura ◽  
T. Copeland ◽  
Y Chen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Oncostatin M ◽  
Sarcoma Cells

Angiogenic properties of Kaposi's sarcoma-derived cells after long-term culture in vitro

Science ◽  
1988 ◽  
Vol 242 (4877) ◽  
pp. 430-433 ◽  
Author(s):  
S. Salahuddin ◽  
S Nakamura ◽  
P Biberfeld ◽  
M. Kaplan ◽  
P. Markham ◽  
...  
Keyword(s):  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Culture In Vitro ◽  
Long Term Culture

scholarly journals Vascular Endothelial Growth Factor-C Stimulates the Migration and Proliferation of Kaposi’s Sarcoma Cells

1999 ◽  
Vol 274 (39) ◽  
pp. 27617-27622 ◽  
Author(s):  
Serena Marchiò ◽  
Luca Primo ◽  
Marco Pagano ◽  
Giorgio Palestro ◽  
Adriana Albini ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Vascular Endothelial ◽  
Factor C ◽  
Sarcoma Cells ◽  
Endothelial Growth Factor

Diacylglycerol kinases are essential for hepatocyte growth factor-dependent proliferation and motility of Kaposi’s sarcoma cells

2011 ◽  
Vol 102 (7) ◽  
pp. 1329-1336 ◽  
Author(s):  
Gianluca Baldanzi ◽  
Stefano Pietronave ◽  
Deborah Locarno ◽  
Simone Merlin ◽  
Paolo Porporato ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Sarcoma Cells ◽  
Hepatocyte Growth

scholarly journals Long-Term-Infected Telomerase-Immortalized Endothelial Cells: a Model for Kaposi's Sarcoma-Associated Herpesvirus Latency In Vitro and In Vivo

2006 ◽  
Vol 80 (10) ◽  
pp. 4833-4846 ◽  
Author(s):  
Feng-Qi An ◽  
Hope Merlene Folarin ◽  
Nicole Compitello ◽  
Justin Roth ◽  
Stanton L. Gerson ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Cell Lines ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Lytic Replication ◽  
Nuclear Antigen ◽  
Kaposi's Sarcoma Associated Herpesvirus ◽  
Kaposi’S Sarcoma Associated Herpesvirus

ABSTRACT Kaposi's sarcoma-associated herpesvirus (KSHV) is associated with Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman's disease. Most KS tumor cells are latently infected with KSHV and are of endothelial origin. While PEL-derived cell lines maintain KSHV indefinitely, all KS tumor-derived cells to date have lost viral genomes upon ex vivo cultivation. To study KSHV latency and tumorigenesis in endothelial cells, we generated telomerase-immortalized human umbilical vein endothelial (TIVE) cells. TIVE cells express all KSHV latent genes 48 h postinfection, and productive lytic replication could be induced by RTA/Orf50. Similar to prior models, infected cultures gradually lost viral episomes. However, we also obtained, for the first time, two endothelial cell lines in which KSHV episomes were maintained indefinitely in the absence of selection. Long-term KSHV maintenance correlated with loss of reactivation in response to RTA/Orf50 and complete oncogenic transformation. Long-term-infected TIVE cells (LTC) grew in soft agar and proliferated under reduced-serum conditions. LTC, but not parental TIVE cells, formed tumors in nude mice. These tumors expressed high levels of the latency-associated nuclear antigen (LANA) and expressed lymphatic endothelial specific antigens as found in KS (LYVE-1). Furthermore, host genes, like those encoding interleukin 6, vascular endothelial growth factor, and basic fibroblast growth factor, known to be highly expressed in KS lesions were also induced in LTC-derived tumors. KSHV-infected LTCs represent the first xenograft model for KS and should be of use to study KS pathogenesis and for the validation of anti-KS drug candidates.

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