Angiogenic properties of Kaposi's sarcoma-derived cells after long-term culture in vitro

Science ◽  
1988 ◽  
Vol 242 (4877) ◽  
pp. 430-433 ◽  
Author(s):  
S. Salahuddin ◽  
S Nakamura ◽  
P Biberfeld ◽  
M. Kaplan ◽  
P. Markham ◽  
...  
Keyword(s):  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Culture In Vitro ◽  
Long Term Culture

Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro

2017 ◽  
Vol 19 (3) ◽  
pp. 159-170 ◽  
Author(s):  
Agnese Gugliandolo ◽  
Thangavelu Soundara Rajan ◽  
Domenico Scionti ◽  
Francesca Diomede ◽  
Placido Bramanti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture In Vitro ◽  
Long Term Culture ◽  
Oncogene Expression

scholarly journals Long-term culture in vitro impairs the immunosuppressive activity of mesenchymal stem cells on T cells

2012 ◽  
Vol 6 (5) ◽  
pp. 1183-1189 ◽  
Author(s):  
XUE-YI LI ◽  
JIN DING ◽  
ZHAO-HUI ZHENG ◽  
XIAO-YAN LI ◽  
ZHEN-BIAO WU ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cells ◽  
Mesenchymal Stem Cells ◽  
Immunosuppressive Activity ◽  
Culture In Vitro ◽  
Long Term Culture

Human placental decidua basalis-derived mesenchymal stem cells differentiate into dopamine neuron-like cells with no response to long-term culture in vitro

Neuroreport ◽  
2012 ◽  
Vol 23 (8) ◽  
pp. 513-518 ◽  
Author(s):  
Guo-hui Lu ◽  
Wang-shi Yong ◽  
Zhi-min Xu ◽  
Yi-quan Ke ◽  
Xiao-dan Jiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dopamine Neuron ◽  
Culture In Vitro ◽  
Long Term Culture ◽  
Decidua Basalis

scholarly journals Changes in mesenchymal stem cells following long-term culture in vitro

2016 ◽  
Vol 13 (6) ◽  
pp. 5207-5215 ◽  
Author(s):  
YAJUN GU ◽  
TAO LI ◽  
YANLING DING ◽  
LINGXIAN SUN ◽  
TAO TU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture In Vitro ◽  
Long Term Culture

Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells

Science ◽  
1988 ◽  
Vol 242 (4877) ◽  
pp. 426-430 ◽  
Author(s):  
S Nakamura ◽  
S. Salahuddin ◽  
P Biberfeld ◽  
B Ensoli ◽  
P. Markham ◽  
...  
Keyword(s):  
T Cells ◽  
Growth Factor ◽  
Cd4 T Cells ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Long Term Culture ◽  
Sarcoma Cells

scholarly journals c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing

2021 ◽  
Vol 30 ◽  
pp. 096368972198960
Author(s):  
Chengcheng Shen ◽  
Yuangang Lu ◽  
Jianghe Zhang ◽  
Yujie Li ◽  
Yiming Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Diabetic Wound ◽  
Culture In Vitro ◽  
Long Term Culture ◽  
Diabetic Wound Healing ◽  
B Lineage

The chronic wound induced by diabetes has poor efficacy and could lead to amputation. The repair function of mesenchymal stem cells (MSCs) impaired after long-term culture in vitro. Studies have shown that the proto-oncogene c-Casitas b-lineage lymphoma (c-Cbl) can regulate receptor- and non-receptor tyrosine kinase, which was also involved in the angiogenesis process. This study aimed to explore the regulative effect of c-Cbl on the proangiogenic functions of long-term cultured MSCs and evaluate its pro-healing effect on diabetic wounds. In this study, the c-Cbl level was downregulated by locked nucleic acid–modified antisense oligonucleotide gapmers (LNA Gapmers). We detected the effect of c-Cbl downregulation on long-term cultured MSCs in terms of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal, cellular proliferation, senescence, migration, and angiogenic factors paracrine activity in vitro. In vivo, we observed the pro-healing effect of long-term cultured MSCs, with or without c-Cbl downregulation, on the diabetic wound. We found that the phosphorylation level of c-Cbl increased and that of Akt decreased in passage 10 (P10) MSCs compared with passage 3 (P3) MSCs ( P < 0.05). Additionally, the proliferation, paracrine, and migration capacity of P10 MSCs decreased significantly, accompanied by the increase of cellular senescence ( P < 0.05). However, these functions, including PI3K/Akt activity of P10 MSCs, have been improved by c-Cbl downregulation ( P < 0.05). Compared with P10 MSCs treatment, treatment with c-Cbl downregulated P10 MSCs accelerated diabetic wound healing, as defined by a more rapid wound closure ( P < 0.05), more neovascularization ( P < 0.05), and higher scores of wound histological assessment ( P < 0.05) in a diabetic rat model. Our findings suggested that c-Cbl downregulation could attenuate the impairment of proangiogenic functions in MSCs induced by long-term culture in vitro and improve the effect of long-term cultured MSCs in promoting diabetic wound healing.

Centrifugal enhancement of Kaposi's sarcoma-associated virus infection of human endothelial cells in vitro

2008 ◽  
Vol 154 (1-2) ◽  
pp. 160-166 ◽  
Author(s):  
Seung-Min Yoo ◽  
Ae-Kyung Ahn ◽  
Taegun Seo ◽  
Hyo Bong Hong ◽  
Myung-Ae Chung ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Virus Infection ◽  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Human Endothelial Cells
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