scholarly journals Asymmetric Division of Drosophila Male Germline Stem Cell Shows Asymmetric Histone Distribution

Science ◽  
2012 ◽  
Vol 338 (6107) ◽  
pp. 679-682 ◽  
Author(s):  
V. Tran ◽  
C. Lim ◽  
J. Xie ◽  
X. Chen
Keyword(s):  
Stem Cell ◽  
Asymmetric Division ◽  
Germline Stem Cell ◽  
Male Germline

scholarly journals Characterization of histone inheritance patterns in the Drosophila female germline

2020 ◽  
Author(s):  
Elizabeth W. Kahney ◽  
Lydia Sohn ◽  
Kayla Viets-Layng ◽  
Robert Johnston ◽  
Xin Chen
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Single Gene ◽  
Asymmetric Division ◽  
Germline Stem Cell ◽  
Inheritance Patterns ◽  
Daughter Cells ◽  
Dividing Cells ◽  
Female Germline

ABSTRACTStem cells have the unique ability to undergo asymmetric division which produces two daughter cells that are genetically identical, but commit to different cell fates. The loss of this balanced asymmetric outcome can lead to many diseases, including cancer and tissue dystrophy. Understanding this tightly regulated process is crucial in developing methods to treat these abnormalities. Here, we report that produced from a Drosophila female germline stem cell asymmetric division, the two daughter cells differentially inherit histones at key genes related to either maintaining the stem cell state or promoting differentiation, but not at constitutively active or silenced genes. We combined histone labeling with DNA Oligopaints to distinguish old versus new histone distribution and visualize their inheritance patterns at single-gene resolution in asymmetrically dividing cells in vivo. This strategy can be widely applied to other biological contexts involving cell fate establishment during development or tissue homeostasis in multicellular organisms.

scholarly journals Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries in Drosophila male germline stem cell division

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Cuie Chen ◽  
Mayu Inaba ◽  
Zsolt G Venkei ◽  
Yukiko M Yamashita
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Cell Fate ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Mother And Daughter ◽  
Stem Cell Divisions ◽  
Stem Cell Division ◽  
Male Germline Stem Cells

Asymmetric stem cell division is often accompanied by stereotypical inheritance of the mother and daughter centrosomes. However, it remains unknown whether and how stem cell centrosomes are uniquely regulated and how this regulation may contribute to stem cell fate. Here we identify Klp10A, a microtubule-depolymerizing kinesin of the kinesin-13 family, as the first protein enriched in the stem cell centrosome in Drosophila male germline stem cells (GSCs). Depletion of klp10A results in abnormal elongation of the mother centrosomes in GSCs, suggesting the existence of a stem cell-specific centrosome regulation program. Concomitant with mother centrosome elongation, GSCs form asymmetric spindle, wherein the elongated mother centrosome organizes considerably larger half spindle than the other. This leads to asymmetric cell size, yielding a smaller differentiating daughter cell. We propose that klp10A functions to counteract undesirable asymmetries that may result as a by-product of achieving asymmetries essential for successful stem cell divisions.

PLZF-Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a

2015 ◽  
Vol 117 (4) ◽  
pp. 844-852 ◽  
Author(s):  
Hailong Mu ◽  
Na Li ◽  
Jiang Wu ◽  
Liming Zheng ◽  
Yuanxin Zhai ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Dairy Goat ◽  
Germline Stem Cell ◽  
Stem Cell Proliferation ◽  
Male Germline

scholarly journals miR‐34c works downstream of p53 leading to dairy goat male germline stem‐cell ( mGSC s) apoptosis

2013 ◽  
Vol 46 (2) ◽  
pp. 223-231 ◽  
Author(s):  
M. Li ◽  
M. Yu ◽  
C. Liu ◽  
H. Zhu ◽  
X. He ◽  
...  
Keyword(s):  
Stem Cell ◽  
Dairy Goat ◽  
Germline Stem Cell ◽  
Male Germline
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