scholarly journals Generating multifunctional acoustic tweezers in Petri dishes for contactless, precise manipulation of bioparticles

2020 ◽  
Vol 6 (37) ◽  
pp. eabb0494 ◽  
Author(s):  
Zhenhua Tian ◽  
Zeyu Wang ◽  
Peiran Zhang ◽  
Ty Downing Naquin ◽  
John Mai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Acoustic Wave ◽  
Three Dimensional ◽  
Petri Dish ◽  
Cell Patterning ◽  
Label Free ◽  
Acoustic Beams ◽  
Resolution Limits ◽  
Petri Dishes ◽  
Acoustic Tweezers

Acoustic tweezers are a promising technology for the biocompatible, precise manipulation of delicate bioparticles ranging from nanometer-sized exosomes to millimeter-sized zebrafish larva. However, their widespread usage is hindered by their low compatibility with the workflows in biological laboratories. Here, we present multifunctional acoustic tweezers that can manipulate bioparticles in a disposable Petri dish. Various functionalities including cell patterning, tissue engineering, concentrating particles, translating cells, stimulating cells, and cell lysis are demonstrated. Moreover, leaky surface acoustic wave–based holography is achieved by encoding required phases in electrode profiles of interdigitated transducers. This overcomes the frequency and resolution limits of previous holographic techniques to control three-dimensional acoustic beams in microscale. This study presents a favorable technique for noncontact and label-free manipulation of bioparticles in commonly used Petri dishes. It can be readily adopted by the biological and medical communities for cell studies, tissue generation, and regenerative medicine.

scholarly journals In Situ Tissue Engineering Using Magnetically Guided Three-Dimensional Cell Patterning

2012 ◽  
Vol 18 (7) ◽  
pp. 496-506 ◽  
Author(s):  
Shawn P. Grogan ◽  
Chantal Pauli ◽  
Peter Chen ◽  
Jiang Du ◽  
Christine B. Chung ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cell Patterning ◽  
Dimensional Cell ◽  
In Situ Tissue Engineering

scholarly journals Wave number–spiral acoustic tweezers for dynamic and reconfigurable manipulation of particles and cells

2019 ◽  
Vol 5 (5) ◽  
pp. eaau6062 ◽  
Author(s):  
Zhenhua Tian ◽  
Shujie Yang ◽  
Po-Hsun Huang ◽  
Zeyu Wang ◽  
Peiran Zhang ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Cell Interaction ◽  
Cell Manipulation ◽  
Wave Number ◽  
Label Free ◽  
Multiple Functions ◽  
Pressure Distributions ◽  
Acoustic Tweezers ◽  
Interaction Research

Acoustic tweezers have recently raised great interest across many fields including biology, chemistry, engineering, and medicine, as they can perform contactless, label-free, biocompatible, and precise manipulation of particles and cells. Here, we present wave number–spiral acoustic tweezers, which are capable of dynamically reshaping surface acoustic wave (SAW) wavefields to various pressure distributions to facilitate dynamic and programmable particle/cell manipulation. SAWs propagating in multiple directions can be simultaneously and independently controlled by simply modulating the multitone excitation signals. This allows for dynamic reshaping of SAW wavefields to desired distributions, thus achieving programmable particle/cell manipulation. We experimentally demonstrated the multiple functions of wave number–spiral acoustic tweezers, among which are multiconfiguration patterning; parallel merging; pattern translation, transformation, and rotation; and dynamic translation of single microparticles along complex paths. This wave number–spiral design has the potential to revolutionize future acoustic tweezers development and advance many applications, including microscale assembly, bioprinting, and cell-cell interaction research.

scholarly journals New approaches for tissue engineering: three dimensional cell patterning using inkjet technology

2008 ◽  
Vol 28 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Chizuka Henmi ◽  
Makoto Nakamura ◽  
Yuichi Nishiyama ◽  
Kumiko Yamaguchi ◽  
Shuichi Mochizuki ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cell Patterning ◽  
New Approaches ◽  
Dimensional Cell

scholarly journals Magnetic levitational bioassembly of 3D tissue construct in space

2020 ◽  
Vol 6 (29) ◽  
pp. eaba4174 ◽  
Author(s):  
Vladislav A. Parfenov ◽  
Yusef D. Khesuani ◽  
Stanislav V. Petrov ◽  
Pavel A. Karalkin ◽  
Elizaveta V. Koudan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Label Free ◽  
Tissue Constructs ◽  
Advanced Stages ◽  
Feasible Alternative ◽  
New Perspective ◽  
First Time ◽  
Conceptual Advance ◽  
Good Agreement

Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a rapidly emerging scaffold- and label-free approach and alternative conceptual advance in tissue engineering. The magnetic bioassembler has been designed, developed, and certified for life space research. To the best of our knowledge, 3D tissue constructs have been biofabricated for the first time in space under microgravity from tissue spheroids consisting of human chondrocytes. Bioassembly and sequential tissue spheroid fusion presented a good agreement with developed predictive mathematical models and computer simulations. Tissue constructs demonstrated good viability and advanced stages of tissue spheroid fusion process. Thus, our data strongly suggest that scaffold-free formative biofabrication using magnetic fields is a feasible alternative to traditional scaffold-based approaches, hinting a new perspective avenue of research that could significantly advance tissue engineering. Magnetic levitational bioassembly in space can also advance space life science and space regenerative medicine.

Üç boyutlu biyo-üretim: İlk izlenimlerimiz ve Çocuk Cerrahisindeki potansiyeli

2020 ◽  
Author(s):  
Emil Mammadov ◽  
Ersin Aytaç ◽  
Ali Türk ◽  
Nurullah Akkaya ◽  
Görkem Say ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pediatric Surgery ◽  
Three Dimensional ◽  
Petri Dish ◽  
Three Dimensions ◽  
Abdominal Wall Defects ◽  
Open Wound ◽  
3D Design ◽  
Stl File ◽  
Computed Tomography Images

Objective: The objective of the study was to design and produce a 3D bioprinter and to evaluate its potential uses in the field of Pediatric Surgery. The design and production of the device whose coordinates could be given with computer-controlled code and having the ability to move in three axes and print the contents of the cartridge on the stationary print bed were realized at the university 3D design and printing laboratories. The Ferret programming language was used for programming and exterior design was made according to the standard laminar cabin dimensions. A 20% gelatin (Sigma Aldrich, MI, USA) solution was used for three-dimensional bioprinting tests. For test bioprints scaffold model used in the field of tissue engineering, for open wound experimental dressing model and for organ-like structures ear model were selected. Scaffold structure was designed with Solidworks (Dassault Systemes,Velizy-Villacoublay, FR) software and open wound experimental dressing model was designed in Fusion360 (Autodesk, CA, USA) software. As an organ-like model, the ear structure has been segmented from the computed tomography images with Synapse3D (Fujifilm, Tokyo, JP) software and converted into “.stl” file. Our device was produced as a machine that can move in the x, y and z axes and can press the sterile syringe contents into the Petri dish in three dimensions. The three-dimensional prints of the scaffold, the experimental wound dressing model obtained from the wound photo and organ model obtained from the CT scan were successfully bioprinted. We think that three-dimensional bioprinters have a great potential in tissue engineering studies in the field of pediatric surgery and will add a brand-new dimension to our research capabilities. We conceive that recurrent cases of hypospadias which especially need tissue reinforcement, diaphragmatic and anterior abdominal wall defects seem to be the primary areas of study.

scholarly journals Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1098
Author(s):  
Yoshitaka Takeuchi ◽  
Shogo Miyata
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cell Patterning ◽  
Mechanical Anisotropy ◽  
Cellular Organization ◽  
Cartilaginous Tissue ◽  
Cell Array ◽  
Organization Level ◽  
Line Array

Recently, many studies have focused on the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three-dimensional (3-D) tissue designed to replace the damaged cartilage. Although tissue engineering is a useful approach to regenerating cartilage, mechanical anisotropy has not been reconstructed from a cellular organization level. This study aims to create mechanically anisotropic cartilaginous tissue using dielectrophoretic cell patterning and gel-sheet lamination. Bovine chondrocytes were patterned in a hydrogel to form line-array cell clusters via negative dielectrophoresis (DEP). The results indicate that the embedded chondrocytes remained viable and reconstructed cartilaginous tissue along the patterned cell array. Moreover, the agarose gel, in which chondrocytes were patterned, demonstrated mechanical anisotropy. In summary, our DEP cell patterning and gel-sheet lamination techniques would be useful for reconstructing mechanically anisotropic cartilage tissues.

scholarly journals Three-dimensional manipulation of single cells using surface acoustic waves

2016 ◽  
Vol 113 (6) ◽  
pp. 1522-1527 ◽  
Author(s):  
Feng Guo ◽  
Zhangming Mao ◽  
Yuchao Chen ◽  
Zhiwei Xie ◽  
James P. Lata ◽  
...  
Keyword(s):  
Acoustic Waves ◽  
Single Cells ◽  
Surface Acoustic Waves ◽  
Three Dimensional ◽  
Biological Cells ◽  
Acoustic Vibrations ◽  
Label Free ◽  
Acoustic Tweezers ◽  
Contact Free ◽  
Micrometer Scale

The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving “acoustic tweezers” in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.

scholarly journals Acoustohydrodynamic tweezers via spatial arrangement of streaming vortices

2021 ◽  
Vol 7 (2) ◽  
pp. eabc7885
Author(s):  
Haodong Zhu ◽  
Peiran Zhang ◽  
Zhanwei Zhong ◽  
Jianping Xia ◽  
Joseph Rich ◽  
...  
Keyword(s):  
Acoustic Radiation ◽  
Acoustic Streaming ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Spatial Arrangement ◽  
Label Free ◽  
Droplet Transport ◽  
Dimensional Boundary ◽  
Acoustic Tweezers ◽  
Contact Free

Acoustics-based tweezers provide a unique toolset for contactless, label-free, and precise manipulation of bioparticles and bioanalytes. Most acoustic tweezers rely on acoustic radiation forces; however, the accompanying acoustic streaming often generates unpredictable effects due to its nonlinear nature and high sensitivity to the three-dimensional boundary conditions. Here, we demonstrate acoustohydrodynamic tweezers, which generate stable, symmetric pairs of vortices to create hydrodynamic traps for object manipulation. These stable vortices enable predictable control of a flow field, which translates into controlled motion of droplets or particles on the operating surface. We built a programmable droplet-handling platform to demonstrate the basic functions of planar-omnidirectional droplet transport, merging droplets, and in situ mixing via a sequential cascade of biochemical reactions. Our acoustohydrodynamic tweezers enables improved control of acoustic streaming and demonstrates a previously unidentified method for contact-free manipulation of bioanalytes and digitalized liquid handling based on a compact and scalable functional unit.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document