scholarly journals Wave number–spiral acoustic tweezers for dynamic and reconfigurable manipulation of particles and cells

2019 ◽  
Vol 5 (5) ◽  
pp. eaau6062 ◽  
Author(s):  
Zhenhua Tian ◽  
Shujie Yang ◽  
Po-Hsun Huang ◽  
Zeyu Wang ◽  
Peiran Zhang ◽  
...  
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Cell Interaction ◽  
Cell Manipulation ◽  
Wave Number ◽  
Label Free ◽  
Multiple Functions ◽  
Pressure Distributions ◽  
Acoustic Tweezers ◽  
Interaction Research

Acoustic tweezers have recently raised great interest across many fields including biology, chemistry, engineering, and medicine, as they can perform contactless, label-free, biocompatible, and precise manipulation of particles and cells. Here, we present wave number–spiral acoustic tweezers, which are capable of dynamically reshaping surface acoustic wave (SAW) wavefields to various pressure distributions to facilitate dynamic and programmable particle/cell manipulation. SAWs propagating in multiple directions can be simultaneously and independently controlled by simply modulating the multitone excitation signals. This allows for dynamic reshaping of SAW wavefields to desired distributions, thus achieving programmable particle/cell manipulation. We experimentally demonstrated the multiple functions of wave number–spiral acoustic tweezers, among which are multiconfiguration patterning; parallel merging; pattern translation, transformation, and rotation; and dynamic translation of single microparticles along complex paths. This wave number–spiral design has the potential to revolutionize future acoustic tweezers development and advance many applications, including microscale assembly, bioprinting, and cell-cell interaction research.

Localized Microstructures Fabrication Through Standing Surface Acoustic Wave and User-Defined Waveguides

2019 ◽  
Author(s):  
Yancheng Wang ◽  
Chenyang Han ◽  
Deqing Mei ◽  
Chengyao Xu
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Structural Design ◽  
Acoustic Waves ◽  
Biomedical Applications ◽  
Cell Interaction ◽  
Experimental Setup ◽  
Acoustic Waveguides ◽  
Novel Method ◽  
Patterned Microstructure

Abstract Polymer-based substrates with patterned microstructure on the surfaces, e.g., cell culturing scaffolds, have been utilized in biomedical applications. This paper develops a novel method to fabricate the localized microstructure on the polymer-based substrate with the assistance of standing surface acoustic wave (SAW) and user-defined acoustic waveguides. The specific designed acoustic waveguides can localize the standing acoustic waves and transmit to the liquid film and excite patterned microstructures on the surface, then using ultraviolet (UV) to solidify the substrate with patterned microstructures. The structural design and fabrication of the SAW device and three different shaped acoustic waveguides are presented. Then, experimental setup and procedures to verify the polymer-substrate with localized microstructures fabrication are performed. By using the different shape of the acoustic waveguides, several types of patterned microstructures with different morphologies are successfully fabricated. Results demonstrated that the proposed fabrication method is an effective way to fabricate polymer-based substrate with localized patterned microstructures, which may have potential in the research on tissue engineering, cell-cell interaction, and other biomedical applications.

scholarly journals Hybrid Sensor Device for Simultaneous Surface Plasmon Resonance and Surface Acoustic Wave Measurements

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6177
Author(s):  
Anastasios G. Samarentsis ◽  
Alexandros K. Pantazis ◽  
Achilleas Tsortos ◽  
Jean-Michel Friedt ◽  
Electra Gizeli
Keyword(s):  
Surface Plasmon Resonance ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Love Wave ◽  
Future Application ◽  
Label Free ◽  
Combined System ◽  
Wave Measurements

Surface plasmon resonance (SPR) and Love wave (LW) surface acoustic wave (SAW) sensors have been established as reliable biosensing technologies for label-free, real-time monitoring of biomolecular interactions. This work reports the development of a combined SPR/LW-SAW platform to facilitate simultaneous optical and acoustic measurements for the investigation of biomolecules binding on a single surface. The system’s output provides recordings of two acoustic parameters, phase and amplitude of a Love wave, synchronized with SPR readings. We present the design and manufacturing of a novel experimental set-up employing, in addition to the SPR/LW-SAW device, a 3D-printed plastic holder combined with a PDMS microfluidic cell so that the platform can be used in a flow-through mode. The system was evaluated in a systematic study of the optical and acoustic responses for different surface perturbations, i.e., rigid mass loading (Au deposition), pure viscous loading (glycerol and sucrose solutions) and protein adsorption (BSA). Our results provide the theoretical and experimental basis for future application of the combined system to other biochemical and biophysical studies.

Microfluidic long-term cell culture platform with cell-cell interaction monitoring using surface acoustic wave

MHS2013 ◽  
2013 ◽  
Author(s):  
Atsushi Takano ◽  
Tasuku Kon ◽  
Yasubumi Furuya ◽  
Katsumi Mochitate ◽  
Masato Tanaka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Cell Interaction ◽  
Cell Cell

A Surface Acoustic Wave Biosensor Concept with Low Flow Cell Volumes for Label-Free Detection

2003 ◽  
Vol 75 (20) ◽  
pp. 5561-5566 ◽  
Author(s):  
Kerstin Länge ◽  
Florian Bender ◽  
Achim Voigt ◽  
Hui Gao ◽  
Michael Rapp
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Flow Cell ◽  
Low Flow ◽  
Label Free ◽  
Label Free Detection ◽  
Acoustic Wave Biosensor ◽  
Cell Volumes

scholarly journals Generating multifunctional acoustic tweezers in Petri dishes for contactless, precise manipulation of bioparticles

2020 ◽  
Vol 6 (37) ◽  
pp. eabb0494 ◽  
Author(s):  
Zhenhua Tian ◽  
Zeyu Wang ◽  
Peiran Zhang ◽  
Ty Downing Naquin ◽  
John Mai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Acoustic Wave ◽  
Three Dimensional ◽  
Petri Dish ◽  
Cell Patterning ◽  
Label Free ◽  
Acoustic Beams ◽  
Resolution Limits ◽  
Petri Dishes ◽  
Acoustic Tweezers

Acoustic tweezers are a promising technology for the biocompatible, precise manipulation of delicate bioparticles ranging from nanometer-sized exosomes to millimeter-sized zebrafish larva. However, their widespread usage is hindered by their low compatibility with the workflows in biological laboratories. Here, we present multifunctional acoustic tweezers that can manipulate bioparticles in a disposable Petri dish. Various functionalities including cell patterning, tissue engineering, concentrating particles, translating cells, stimulating cells, and cell lysis are demonstrated. Moreover, leaky surface acoustic wave–based holography is achieved by encoding required phases in electrode profiles of interdigitated transducers. This overcomes the frequency and resolution limits of previous holographic techniques to control three-dimensional acoustic beams in microscale. This study presents a favorable technique for noncontact and label-free manipulation of bioparticles in commonly used Petri dishes. It can be readily adopted by the biological and medical communities for cell studies, tissue generation, and regenerative medicine.

Sign in / Sign up

Export Citation Format

Share Document