Üç boyutlu biyo-üretim: İlk izlenimlerimiz ve Çocuk Cerrahisindeki potansiyeli

2020 ◽  
Author(s):  
Emil Mammadov ◽  
Ersin Aytaç ◽  
Ali Türk ◽  
Nurullah Akkaya ◽  
Görkem Say ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pediatric Surgery ◽  
Three Dimensional ◽  
Petri Dish ◽  
Three Dimensions ◽  
Abdominal Wall Defects ◽  
Open Wound ◽  
3D Design ◽  
Stl File ◽  
Computed Tomography Images

Objective: The objective of the study was to design and produce a 3D bioprinter and to evaluate its potential uses in the field of Pediatric Surgery. The design and production of the device whose coordinates could be given with computer-controlled code and having the ability to move in three axes and print the contents of the cartridge on the stationary print bed were realized at the university 3D design and printing laboratories. The Ferret programming language was used for programming and exterior design was made according to the standard laminar cabin dimensions. A 20% gelatin (Sigma Aldrich, MI, USA) solution was used for three-dimensional bioprinting tests. For test bioprints scaffold model used in the field of tissue engineering, for open wound experimental dressing model and for organ-like structures ear model were selected. Scaffold structure was designed with Solidworks (Dassault Systemes,Velizy-Villacoublay, FR) software and open wound experimental dressing model was designed in Fusion360 (Autodesk, CA, USA) software. As an organ-like model, the ear structure has been segmented from the computed tomography images with Synapse3D (Fujifilm, Tokyo, JP) software and converted into “.stl” file. Our device was produced as a machine that can move in the x, y and z axes and can press the sterile syringe contents into the Petri dish in three dimensions. The three-dimensional prints of the scaffold, the experimental wound dressing model obtained from the wound photo and organ model obtained from the CT scan were successfully bioprinted. We think that three-dimensional bioprinters have a great potential in tissue engineering studies in the field of pediatric surgery and will add a brand-new dimension to our research capabilities. We conceive that recurrent cases of hypospadias which especially need tissue reinforcement, diaphragmatic and anterior abdominal wall defects seem to be the primary areas of study.

Development of Scaffold Building Units and Assembly for Tissue Engineering Using Fused Deposition Modelling

2009 ◽  
Vol 83-86 ◽  
pp. 269-274 ◽  
Author(s):  
Syed H. Masood ◽  
Kadhim Alamara
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Fused Deposition Modeling ◽  
Porous Scaffold ◽  
Cell Structure ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Fused Deposition Modelling ◽  
Fused Deposition ◽  
Unit Cells

In tissue engineering (TE), a porous scaffold structure of biodegradable material is required as a template to guide the proliferation, growth and development of cells appropriately in three dimensions. The scaffold must meet design requirements of appropriate porosity, pore size and interconnected structure to allow cell proliferation and adhesion. This paper presents a methodology for design and manufacture of TE scaffolds with varying porosity by employing open structure building units and Fused Deposition Modeling (FDM) rapid prototyping technique. A computer modeling approach for constructing and assembly of three-dimensional unit cell structure is presented to provide a solution of scaffolds design that can potentially meet the diverse requirements of TE applications. A parametric set of open polyhedral unit cells is used to assist the user in designing the required micro-architecture of the scaffold with required porosity and pore size and then the Boolean operation is used to create the scaffold of a given CAD model from the designed microstructure. The procedure is verified by fabrication of physical scaffolds using the commercial FDM system.

Cholesteatoma in three dimensions: a teaching tool and an aid to improved pre-operative consent

2009 ◽  
Vol 124 (2) ◽  
pp. 126-131 ◽  
Author(s):  
D P Morris ◽  
R G Van Wijhe
Keyword(s):  
Temporal Bone ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Teaching Tool ◽  
Surgical Experience ◽  
Teaching Programme ◽  
Computed Tomography Images ◽  
Three Dimensional Models ◽  
Temporal Bones ◽  
User Friendly

AbstractBackground:Otological surgeons face two recurring challenges. Firstly, we must foster an appreciation of the complex, three-dimensional anatomy of the temporal bone in order to enable our trainees to operate safely and independently. Secondly, we must explain to our patients the necessity for surgery which carries the potential for serious complication.Methods:Amira® software was applied to pre-operative computed tomography images of temporal bones with cholesteatoma, to create three-dimensional computer images. Normal structures and cholesteatoma were displayed in a user-friendly, interactive format, allowing both trainee and patient to visualise disease and important structures within the temporal bone.Results:Three cases, and their three-dimensional computer models are presented. Zoom, rotation and transparency functions complemented the three-dimensional effect.Conclusion:These three-dimensional models provided a useful adjunct to cadaveric temporal bone dissection and surgical experience for our residents' teaching programme. Also, patients with cholesteatoma reported a better understanding of their pre-operative condition when the models were used during the consenting process.

scholarly journals Quantification of Contralateral Differences of the Scaphoid: A Comparison of Bone Geometry in Three Dimensions

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Claudio Letta ◽  
Andreas Schweizer ◽  
Philipp Fürnstahl
Keyword(s):  
Surface Area ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Bone Geometry ◽  
Statistical Correlation ◽  
Three Dimensions ◽  
Computed Tomography Images ◽  
Surface Models ◽  
Surface Deviation ◽  
The Right

The purpose of this study was to accurately quantify contralateral differences of the scaphoid in three-dimensional space to evaluate the feasibility of using the healthy contralateral bone as a reconstruction template in the preoperative planning of complex mal- or nonunions. Three-dimensional surface models of the left and right scaphoids were reconstructed from computed tomography images and compared in 26 individuals. Left-right differences were quantified with respect to volume, surface area, length, and surface-to-surface deviation. The average left-right differences in volume, surface area, and length were 95.4 mm3 (SD 66.2 mm3), 32.7 mm2 (SD 22.9 mm32), and 0.28 mm (SD 0.4 mm), respectively. The average surface-to-surface deviation between the sides was 0.26 mm (SD 0.2 mm). High statistical correlation (Pearson) between the left and the right side was found in all evaluated measures.

scholarly journals Generating multifunctional acoustic tweezers in Petri dishes for contactless, precise manipulation of bioparticles

2020 ◽  
Vol 6 (37) ◽  
pp. eabb0494 ◽  
Author(s):  
Zhenhua Tian ◽  
Zeyu Wang ◽  
Peiran Zhang ◽  
Ty Downing Naquin ◽  
John Mai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Acoustic Wave ◽  
Three Dimensional ◽  
Petri Dish ◽  
Cell Patterning ◽  
Label Free ◽  
Acoustic Beams ◽  
Resolution Limits ◽  
Petri Dishes ◽  
Acoustic Tweezers

Acoustic tweezers are a promising technology for the biocompatible, precise manipulation of delicate bioparticles ranging from nanometer-sized exosomes to millimeter-sized zebrafish larva. However, their widespread usage is hindered by their low compatibility with the workflows in biological laboratories. Here, we present multifunctional acoustic tweezers that can manipulate bioparticles in a disposable Petri dish. Various functionalities including cell patterning, tissue engineering, concentrating particles, translating cells, stimulating cells, and cell lysis are demonstrated. Moreover, leaky surface acoustic wave–based holography is achieved by encoding required phases in electrode profiles of interdigitated transducers. This overcomes the frequency and resolution limits of previous holographic techniques to control three-dimensional acoustic beams in microscale. This study presents a favorable technique for noncontact and label-free manipulation of bioparticles in commonly used Petri dishes. It can be readily adopted by the biological and medical communities for cell studies, tissue generation, and regenerative medicine.

convergent-beam diffraction from surfaces

1987 ◽  
Vol 45 ◽  
pp. 30-33
Author(s):  
J. A. Eades ◽  
A. E. Smith ◽  
D. F. Lynch
Keyword(s):  
Reciprocal Lattice ◽  
Three Dimensional ◽  
Grazing Incidence ◽  
Three Dimensions ◽  
Plane Figure ◽  
Transmission Electron ◽  
Convergent Beam ◽  
Beam Patterns ◽  
Beam Diffraction

It is quite simple (in the transmission electron microscope) to obtain convergent-beam patterns from the surface of a bulk crystal. The beam is focussed onto the surface at near grazing incidence (figure 1) and if the surface is flat the appropriate pattern is obtained in the diffraction plane (figure 2). Such patterns are potentially valuable for the characterization of surfaces just as normal convergent-beam patterns are valuable for the characterization of crystals.There are, however, several important ways in which reflection diffraction from surfaces differs from the more familiar electron diffraction in transmission.GeometryIn reflection diffraction, because of the surface, it is not possible to describe the specimen as periodic in three dimensions, nor is it possible to associate diffraction with a conventional three-dimensional reciprocal lattice.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

Extension of the Spatial PDI Model to Three Dimensions

1997 ◽  
Vol 84 (1) ◽  
pp. 176-178
Author(s):  
Frank O'Brien
Keyword(s):  
Population Density ◽  
Dimensional Space ◽  
Finite Lattice ◽  
Three Dimensional ◽  
Upper Bounds ◽  
Three Dimensions ◽  
Lower And Upper Bounds ◽  
Density Index ◽  
Three Dimensional Space

The author's population density index ( PDI) model is extended to three-dimensional distributions. A derived formula is presented that allows for the calculation of the lower and upper bounds of density in three-dimensional space for any finite lattice.

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

scholarly journals A CBCT Based Three-Dimensional Assessment of Mandibular Posterior Region for Evaluating the Possibility of Bypassing the Inferior Alveolar Nerve While Placing Dental Implants

Diagnostics ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 406 ◽  
Author(s):  
Mohammed G. Sghaireen ◽  
Kumar Chandan Srivastava ◽  
Deepti Shrivastava ◽  
Kiran Kumar Ganji ◽  
Santosh R. Patil ◽  
...  
Keyword(s):  
Nerve Injury ◽  
Age Groups ◽  
Inferior Alveolar Nerve ◽  
Three Dimensions ◽  
High Rate ◽  
Cortical Plate ◽  
Computed Tomography Images ◽  
Group I ◽  
Linear Distance ◽  
The Right

A high rate of nerve injury and related consequences are seen during implant placement in the posterior mandibular arch. An approach has been proposed to avoid nerve injury by dodging the inferior alveolar nerve (IAN) while placing an implant. A prospective study with a total of 240 CBCT (cone beam computed tomography) images of patients with three dentate statuses, namely, edentulous (group I), partially edentulous (group II) and dentate (group III) were included in the study. The nerve path tracing was done on CBCT images with On-demand 3D software. The three dimensions, i.e., the linear distance from the outer buccal cortical plate to the inferior alveolar nerve (BCPN), linear distance from the outer lingual cortical plate to the inferior alveolar nerve (LCPN) and linear distance from the midpoint of the alveolar crest to the inferior alveolar nerve (ACN) were assessed. The data were presented and analyzed between variables using one-way ANOVA and independent t-test in SPSS version 21.LCPN of the right 1st premolar region (p < 0.05) was significantly different among the groups with edentulous subjects recorded with the minimum value (6.50 ± 1.20 mm). Females were found to have significantly (p < 0.05) less available bone (6.03 ± 1.46 mm) on the right side of the mandibular jaw compared to males in edentulous group of patients. On comparing age groups for partially edentulous subjects, LCPN of the right 1st premolar region had significantly (p < 0.05) less available bone (6.03 ± 0.38 mm) in subjects with age ≥54 years. The IAN follows a lingual course in the molar region and later flips to the buccal side in the premolar region. The LCPN dimension in the 1st and 2nd premolar region was found to be more than 6 mm irrespective of age, gender and side of the jaw. Thus, it can be considered as a suitable site for placing implants while bypassing the IAN with CBCT assessment remaining as the mainstay in the pre-surgical phase.

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