scholarly journals Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling

2020 ◽  
Vol 6 (24) ◽  
pp. eaba7602
Author(s):  
Ziad Julier ◽  
Rezvan Karami ◽  
Bhavana Nayer ◽  
Yen-Zhen Lu ◽  
Anthony J. Park ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Growth Factors ◽  
Regenerative Medicine ◽  
Bone Regeneration ◽  
Interleukin 1 ◽  
Delivery Systems ◽  
Regenerative Capacity ◽  
Local Inhibition ◽  
Signaling Dynamics ◽  
Regenerative Activity

Although growth factors (GFs) are key molecules for regenerative medicine, their use has been limited by issues associated with suboptimal delivery systems and incomplete understanding of their signaling dynamics. Here, we explored how proinflammatory signals affect GF regenerative potential. Using bone regeneration in mouse, we found that the regenerative capacity of two clinically relevant GFs (BMP-2 and PDGF-BB) is impaired by interleukin-1 receptor (IL-1R1). Mechanistically, IL-1R1 activation in bone-forming cells desensitizes them to GFs and accelerates senescence. Moreover, administration of the GFs triggers IL-1 release by macrophages. To provide localized and sustained IL-1R1 inhibition, we engineered IL-1R antagonist (IL-1Ra) to bind the extracellular matrix (ECM) very strongly and demonstrate that codelivering GFs with ECM-binding IL-1Ra induces superior regeneration. Thus, we highlight that GF regenerative activity is hindered by proinflammatory signals, and GF-based therapies should integrate immunomodulation. Particularly, ECM-binding IL-1Ra holds clinical translational potential by enhancing efficacy of GF therapies.

scholarly journals Delivery Systems and Role of Growth Factors for Alveolar Bone Regeneration in Dentistry

10.5772/55580 ◽  
2013 ◽  
Author(s):  
Stefano Sivolella ◽  
Marleen De ◽  
Giulia Brunello ◽  
Sara Ricci ◽  
Drazen Tadic ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bone Regeneration ◽  
Alveolar Bone ◽  
Delivery Systems

Extracellular matrix-inspired growth factor delivery systems for bone regeneration

2015 ◽  
Vol 94 ◽  
pp. 41-52 ◽  
Author(s):  
Mikaël M. Martino ◽  
Priscilla S. Briquez ◽  
Kenta Maruyama ◽  
Jeffrey A. Hubbell
Keyword(s):  
Extracellular Matrix ◽  
Growth Factor ◽  
Bone Regeneration ◽  
Delivery Systems ◽  
Growth Factor Delivery

scholarly journals Colloidal Gels with Extracellular Matrix Particles and Growth Factors for Bone Regeneration in Critical Size Rat Calvarial Defects

2017 ◽  
Vol 19 (3) ◽  
pp. 703-711 ◽  
Author(s):  
Jakob M. Townsend ◽  
S. Connor Dennis ◽  
Jonathan Whitlow ◽  
Yi Feng ◽  
Jinxi Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Growth Factors ◽  
Bone Regeneration ◽  
Critical Size ◽  
Colloidal Gels ◽  
Calvarial Defects

Microspheres Embedded Hydrogel Construct - Binary Delivery of Alendronate and BMP-2 for Superior Bone Regeneration

2021 ◽  
Author(s):  
Sayanti Datta ◽  
Arun Prabhu Rameshbabu ◽  
Kamakshi Bankoti ◽  
Subhodeep Jana ◽  
Sabyasachi Roy ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Growth Factors ◽  
Bone Regeneration ◽  
Interesting Approach

Biomimetic delivery of osteoinductive growth factors via an osteoconductive matrix is an interesting approach for stimulating bone regeneration. In this context, bone extracellular matrix (ECM) has been explored as an...

scholarly journals Biodegradable Polymers as Drug Delivery Systems for Bone Regeneration

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 95 ◽  
Author(s):  
Kaoru Aoki ◽  
Naoto Saito
Keyword(s):  
Drug Delivery ◽  
Regenerative Medicine ◽  
Bone Regeneration ◽  
Drug Delivery Systems ◽  
Calcium Phosphates ◽  
Delivery Systems ◽  
Polymer Scaffolds ◽  
Synthetic Scaffold ◽  
And Performance ◽  
Potential Use

Regenerative medicine has been widely researched for the treatment of bone defects. In the field of bone regenerative medicine, signaling molecules and the use of scaffolds are of particular importance as drug delivery systems (DDS) or carriers for cell differentiation, and various materials have been explored for their potential use. Although calcium phosphates such as hydroxyapatite and tricalcium phosphate are clinically used as synthetic scaffold material for bone regeneration, biodegradable materials have attracted much attention in recent years for their clinical application as scaffolds due their ability to facilitate rapid localized absorption and replacement with autologous bone. In this review, we introduce the types, features, and performance characteristics of biodegradable polymer scaffolds in their role as DDS for bone regeneration therapy.

scholarly journals Platelet-Rich Plasma in Bone Regeneration: Engineering the Delivery for Improved Clinical Efficacy

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Isaac A. Rodriguez ◽  
Emily A. Growney Kalaf ◽  
Gary L. Bowlin ◽  
Scott A. Sell
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Bone Regeneration ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Clinical Use ◽  
Regenerative Capacity ◽  
Effective Delivery

Human bone is a tissue with a fairly remarkable inherent capacity for regeneration; however, this regenerative capacity has its limitations, and defects larger than a critical size lack the ability to spontaneously heal. As such, the development and clinical translation of effective bone regeneration modalities are paramount. One regenerative medicine approach that is beginning to gain momentum in the clinical setting is the use of platelet-rich plasma (PRP). PRP therapy is essentially a method for concentrating platelets and their intrinsic growth factors to stimulate and accelerate a healing response. While PRP has shown some efficacy in bothin vitroandin vivoscenarios, to date its use and delivery have not been optimized for bone regeneration. Issues remain with the effective delivery of the platelet-derived growth factors to a localized site of injury, the activation and temporal release of the growth factors, and the rate of growth factor clearance. This review will briefly describe the physiological principles behind PRP use and then discuss how engineering its method of delivery may ultimately impact its ability to successfully translate to widespread clinical use.

scholarly journals The Effect of PEI and PVP-Stabilized Gold Nanoparticles on Equine Platelets Activation: Potential Application in Equine Regenerative Medicine

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Mateusz Hecold ◽  
Roma Buczkowska ◽  
Aleksandra Mucha ◽  
Jakub Grzesiak ◽  
Olga Rac-Rumijowska ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Growth Factors ◽  
Growth Factor ◽  
Regenerative Medicine ◽  
Chemical Reduction ◽  
Interleukin 1 ◽  
Mrna Level ◽  
Bone Morphogenetic Protein 2 ◽  
Chloroauric Acid ◽  
Vis Spectroscopy

The aim of this work was to assess the effect of different stabilizing agents, for example, polyethylenimine (PEI) and polyvinylpyrrolidone (PVP), on gold nanoparticles (AuNPs) and their influence on equine platelet activation and release of particular growth factors. The gold nanoparticles were produced by chemical reduction of chloroauric acid. UV-Vis spectroscopy confirmed the presence of gold nanoparticles in investigated solutions. The AuNPs were incubated with whole blood at various concentrations. The morphology of platelets in PRP prepared from the blood incubated with AuNPs was characterized by scanning transmission electron microscopy, whereas the concentrations of growth factors and cytokines were evaluated by ELISA assays. The most promising results were obtained with equine platelets incubated with 5% AuNPs stabilized by PEI, which lead to secretion of bone morphogenetic protein 2 (BMP-2), vascular endothelial growth factor (VEGF), and fibroblast growth factor 1 (FGF-1) and simultaneously cause decrease in concentration of interleukin-1 alpha (IL-1α). The qRT-PCR confirmed ELISA test results. The incubation with 5% AuNPs stabilized by PEI leads to upregulation of BMP-2 and VEGF transcripts of mRNA level and to downregulating expression of interleukin-6 (IL-6). Obtained data shed a promising light on gold nanoparticle application for future regenerative medicine application.

scholarly journals Control of the skin scarring response

2009 ◽  
Vol 81 (3) ◽  
pp. 623-629 ◽  
Author(s):  
Lydia M. Ferreira ◽  
Alfredo Gragnani ◽  
Fabianne Furtado ◽  
Bernardo Hochman
Keyword(s):  
Extracellular Matrix ◽  
Growth Factors ◽  
Regenerative Medicine ◽  
Tissue Repair ◽  
Control Mechanism ◽  
Healing Process ◽  
Multiple Functions ◽  
Matrix Deposition ◽  
Adrenal Axis ◽  
Extracellular Matrix Deposition

There comes a time when the understanding of the cutaneous healing process becomes essential due to the need for a precocious tissue repair to reduce the physical, social, and psychological morbidity. Advances in the knowledge on the control of interaction among cells, matrix and growth factors will provide more information on the Regenerative Medicine, an emerging area of research in medical bioengineering. However, considering the dynamism and complexity of the cutaneous healing response, it is fundamental to understand the control mechanism exerted by the interaction and synergism of both systems, cutaneous nervous and central nervous, via hypothalamus hypophysis-adrenal axis, a relevant subject, but hardly ever explored. The present study reviews the neuro-immune-endocrine physiology of the skin responsible for its multiple functions and the extreme disturbances of the healing process, like the excess and deficiency of the extracellular matrix deposition.

scholarly journals Applications of Carbon Nanotubes in Bone Regenerative Medicine

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 659 ◽  
Author(s):  
Manabu Tanaka ◽  
Kaoru Aoki ◽  
Hisao Haniu ◽  
Takayuki Kamanaka ◽  
Takashi Takizawa ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotubes ◽  
Growth Factors ◽  
Regenerative Medicine ◽  
Bone Regeneration ◽  
Chemical Properties ◽  
Clinical Applications ◽  
Review Article ◽  
Cell Affinity ◽  
Nano Size

Scaffolds are essential for bone regeneration due to their ability to maintain a sustained release of growth factors and to provide a place where cells that form new bone can enter and proliferate. In recent years, scaffolds made of various materials have been developed and evaluated. Functionally effective scaffolds require excellent cell affinity, chemical properties, mechanical properties, and safety. Carbon nanotubes (CNTs) are fibrous nanoparticles with a nano-size diameter and have excellent strength and chemical stability. In the industrial field, they are used as fillers to improve the performance of materials. Because of their excellent physicochemical properties, CNTs are studied for their promising clinical applications as biomaterials. In this review article, we focused on the results of our research on CNT scaffolds for bone regeneration, introduced the promising properties of scaffolds for bone regeneration, and described the potential of CNT scaffolds.

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