scholarly journals A highly sensitive and selective nanosensor for near-infrared potassium imaging

2020 ◽  
Vol 6 (16) ◽  
pp. eaax9757 ◽  
Author(s):  
Jianan Liu ◽  
Limin Pan ◽  
Chunfeng Shang ◽  
Bin Lu ◽  
Rongjie Wu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Cultured Cells ◽  
Mesoporous Silica Nanoparticles ◽  
Living Cells ◽  
Biological Processes ◽  
Intact Mouse ◽  
Selective Filter ◽  
Filter Membrane ◽  
Highly Sensitive ◽  
Hollow Cavity

Potassium ion (K+) concentration fluctuates in various biological processes. A number of K+ probes have been developed to monitor such fluctuations through optical imaging. However, the currently available K+ probes are far from being sensitive enough in detecting physiological fluctuations in living animals. Furthermore, the monitoring of deep tissues is not applicable because of short-wavelength excitation prevailingly used so far. Here, we report a highly sensitive and selective nanosensor for near-infrared (NIR) K+ imaging in living cells and animals. The nanosensor is constructed by encapsulating upconversion nanoparticles (UCNPs) and a commercial K+ indicator in the hollow cavity of mesoporous silica nanoparticles, followed by coating a K+-selective filter membrane. The membrane adsorbs K+ from the medium and filters out interfering cations. The UCNPs convert NIR to ultraviolet light, which excites the K+ indicator, thus allowing the detection of the fluctuations of K+ concentration in cultured cells and intact mouse brains.

A near-infrared ratiometric fluorescent probe for rapid and highly sensitive imaging of endogenous hydrogen sulfide in living cells

2013 ◽  
Vol 4 (6) ◽  
pp. 2551 ◽  
Author(s):  
Xu Wang ◽  
Juan Sun ◽  
Weihong Zhang ◽  
Xiaoxu Ma ◽  
Jianzheng Lv ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Highly Sensitive ◽  
Ratiometric Fluorescent Probe

A near-infrared fluorescent aza-bodipy probe for dual-wavelength detection of hydrogen peroxide in living cells

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2380-2383 ◽  
Author(s):  
Jingjing Xu ◽  
Jingying Zhai ◽  
Yanmei Xu ◽  
Jingwei Zhu ◽  
Yu Qin ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Boronic Acid ◽  
Near Infrared ◽  
High Selectivity ◽  
Living Cells ◽  
Dual Wavelength ◽  
Biological Processes ◽  
Wavelength Detection ◽  
Intracellular Hydrogen Peroxide

A boronic acid functionalized aza-borondipyrromethene dye (azaBDPBA) was applied to the dual-wavelength detection of hydrogen peroxide with high selectivity, which was loaded into cells to indicate the alteration of intracellular hydrogen peroxide during biological processes.

A highly sensitive near-infrared fluorescent probe for cysteine and homocysteine in living cells

2013 ◽  
Vol 49 (80) ◽  
pp. 9176 ◽  
Author(s):  
Fanpeng Kong ◽  
Renpu Liu ◽  
Ranran Chu ◽  
Xu Wang ◽  
Kehua Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Highly Sensitive

A lysosome-targeting viscosity-sensitive fluorescent probe based on a novel functionalised near-infrared xanthene-indolium dye and its application in living cells

2020 ◽  
Vol 8 (38) ◽  
pp. 8838-8844
Author(s):  
Chang Liu ◽  
Tong Zhao ◽  
Song He ◽  
Liancheng Zhao ◽  
Xianshun Zeng
Keyword(s):  
Fluorescent Probe ◽  
Significant Role ◽  
Near Infrared ◽  
Living Cells ◽  
Biological Processes ◽  
The Status ◽  
And Function

The viscosity of lysosomes plays a significant role in modulating biological processes and reflects the status and function of this kind of organelle, e.g., locations, morphologies, and components.

A highly sensitive near-infrared fluorescent probe for the detection of hydrogen sulfide and its application in living cells and mice

2018 ◽  
Vol 42 (24) ◽  
pp. 19795-19800 ◽  
Author(s):  
Xin Zhang ◽  
Runing Sun ◽  
Guofeng Duan ◽  
Ziyan Zhou ◽  
Yanjun Luo ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Living Cells ◽  
Signalling Molecule ◽  
Highly Sensitive ◽  
Biochemical Research

Hydrogen sulfide (H2S), an endogenous gaseous signalling molecule, has attracted attention in biochemical research.

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

A mitochondria-targeted near-infrared fluorescent probe for imaging viscosity in living cells and a diabetic mice model

2021 ◽  
Author(s):  
Bochao Chen ◽  
Shumei Mao ◽  
Yanyan Sun ◽  
Liyuan Sun ◽  
Ning Ding ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Diabetic Mouse ◽  
Living Cells ◽  
Pancreatic Tissue ◽  
Diabetic Mice ◽  
Mice Model ◽  
Viscosity Changes

A mitochondria-targeted near-infrared fluorescent probe NIR-V with 700 nm emission was designed to monitor cell viscosity changes, which was applied to detect the intracellular viscosity and imagine pancreatic tissue in diabetic mouse model.

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