High Affinity Glutamate Transporters: Regulation of Expression and Activity

1997 ◽  
Vol 52 (1) ◽  
pp. 6-15 ◽  
Author(s):  
Georgi Gegelashvili ◽  
Arne Schousboe
Keyword(s):  
Glutamate Transporters ◽  
Regulation Of Expression ◽  
High Affinity ◽  
Expression And Activity

Developmental expression and activity of high affinity glutamate transporters in rat cortical primary cultures

2002 ◽  
Vol 40 (7) ◽  
pp. 661-671 ◽  
Author(s):  
B Guillet ◽  
S Lortet ◽  
F Masmejean ◽  
D Samuel ◽  
A Nieoullon ◽  
...  
Keyword(s):  
Primary Cultures ◽  
Glutamate Transporters ◽  
Developmental Expression ◽  
High Affinity ◽  
Expression And Activity

Differentiation of Substrate and Nonsubstrate Inhibitors of the High-Affinity, Sodium-Dependent Glutamate Transporters

1999 ◽  
Vol 56 (6) ◽  
pp. 1095-1104 ◽  
Author(s):  
Hans P. Koch ◽  
Michael P. Kavanaugh ◽  
Christopher S. Esslinger ◽  
Noah Zerangue ◽  
John M. Humphrey ◽  
...  
Keyword(s):  
Glutamate Transporters ◽  
High Affinity ◽  
Sodium Dependent

scholarly journals In Vivo and In Vitro Evidence that TtgV Is the Specific Regulator of the TtgGHI Multidrug and Solvent Efflux Pump of Pseudomonas putida

2003 ◽  
Vol 185 (16) ◽  
pp. 4755-4763 ◽  
Author(s):  
Antonia Rojas ◽  
Ana Segura ◽  
María Eugenia Guazzaroni ◽  
Wilson Terán ◽  
Ana Hurtado ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Aromatic Hydrocarbons ◽  
Efflux Pump ◽  
Mobility Shift ◽  
Regulation Of Expression ◽  
High Affinity ◽  
Induced Tolerance ◽  
Background Expression

ABSTRACT The TtgGHI efflux pump of Pseudomonas putida DOT-T1E plays a key role in the innate and induced tolerance of this strain to aromatic hydrocarbons and antibiotics. The ttgGHI operon is expressed constitutively from two overlapping promoters in the absence of solvents and at a higher level in their presence, but not in response to antibiotics. Adjacent to the ttgGHI operon is the divergently transcribed ttgVW operon. In TtgV-deficient backgrounds, although not in a TtgW-deficient background, expression of the ttgGHI and ttgVW operons increased fourfold. This suggests that TtgV represses expression from the ttgG promoters and controls its own. TtgW plays no major role in the regulation of expression of these promoters. Primer extension revealed that the divergent ttgG and ttgV promoters overlap, and mobility shift assays indicated that TtgV binds to this region with high affinity. DNaseI footprint assays revealed that TtgV protected four DNA helical turns that include the −10 and −35 boxes of the ttgV and ttgG promoters.

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