Kinetics of 76Br-labeled anti-CEA antibodies in pigs; Aspects of dosimetry and PET imaging properties

1999 ◽  
Vol 26 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Anna Lövqvist ◽  
Hans Lundqvist ◽  
Mark Lubberink ◽  
Vladimir Tolmachev ◽  
Jörgen Carlsson ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Imaging Properties ◽  
Kinetics Of

Pharmacokinetics and PET imaging properties of two recombinant anti-PSMA antibody fragments in comparison to their parental antibody

The Prostate ◽  
2014 ◽  
Vol 74 (7) ◽  
pp. 743-755 ◽  
Author(s):  
Stefan Wiehr ◽  
Patrick Bühler ◽  
Dorothee Gierschner ◽  
Philipp Wolf ◽  
Anna-Maria Rolle ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Antibody Fragments ◽  
Parental Antibody ◽  
Imaging Properties

scholarly journals Increase in negative charge of 68Ga/chelator complex reduces unspecific hepatic uptake but does not improve imaging properties of HER3-targeting affibody molecules

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sara S. Rinne ◽  
Charles Dahlsson Leitao ◽  
Joshua Gentry ◽  
Bogdan Mitran ◽  
Ayman Abouzayed ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Negative Charge ◽  
Human Cancer ◽  
Hepatic Uptake ◽  
Common Mechanism ◽  
Affibody Molecules ◽  
Her3 Expression ◽  
Gallium 68 ◽  
Imaging Properties

AbstractUpregulation of the human epidermal growth factor receptor type 3 (HER3) is a common mechanism to bypass HER-targeted cancer therapy. Affibody-based molecular imaging has the potential for detecting and monitoring HER3 expression during treatment. In this study, we compared the imaging properties of newly generated 68Ga-labeled anti-HER3 affibody molecules (HE)3-ZHER3-DOTA and (HE)3-ZHER3-DOTAGA with previously reported [68Ga]Ga-(HE)3-ZHER3-NODAGA. We hypothesized that increasing the negative charge of the gallium-68/chelator complex would reduce hepatic uptake, which could lead to improved contrast of anti-HER3 affibody-based PET-imaging of HER3 expression. (HE)3-ZHER3-X (X = DOTA, DOTAGA) were produced and labeled with gallium-68. Binding of the new conjugates was specific in HER3 expressing BxPC-3 and DU145 human cancer cells. Biodistribution and in vivo specificity was studied in BxPC-3 xenograft bearing Balb/c nu/nu mice 3 h pi. DOTA- and DOTAGA-containing conjugates had significantly higher concentration in blood than [68Ga]Ga-(HE)3-ZHER3-NODAGA. Presence of the negatively charged 68Ga-DOTAGA complex reduced the unspecific hepatic uptake, but did not improve overall biodistribution of the conjugate. [68Ga]Ga-(HE)3-ZHER3-DOTAGA and [68Ga]Ga-(HE)3-ZHER3-NODAGA had similar tumor-to-liver ratios, but [68Ga]Ga-(HE)3-ZHER3-NODAGA had the highest tumor uptake and tumor-to-blood ratio among the tested conjugates. In conclusion, [68Ga]Ga-(HE)3-ZHER3-NODAGA remains the favorable variant for PET imaging of HER3 expression.

scholarly journals Modeling of the Renal Kinetics of the AT1 Receptor Specific PET Radioligand [11C]KR31173

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Nedim C. M. Gulaldi ◽  
Jinsong Xia ◽  
Tao Feng ◽  
Kelvin Hong ◽  
William B. Mathews ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Compartmental Model ◽  
Radioligand Binding ◽  
Left Kidney ◽  
Binding Parameters ◽  
Retention Parameter ◽  
Time Activity ◽  
Kinetics Of

Purpose. The radioligand [11C]KR31173 has been introduced for PET imaging of the angiotensin II subtype 1 receptor (AT1R). The purpose of the present project was to employ and validate a compartmental model for quantification of the kinetics of this radioligand in a porcine model of renal ischemia followed by reperfusion (IR).Procedures. Ten domestic pigs were included in the study: five controls and five experimental animals with IR of the left kidney. To achieve IR, acute ischemia was created with a balloon inserted into the left renal artery and inflated for 60 minutes. Reperfusion was achieved by deflation and removal of the balloon. Blood chemistries, urine specific gravity and PH values, and circulating hormones of the renin angiotensin system were measured and PET imaging was performed one week after IR. Cortical time-activity curves obtained from a 90 min [11C]KR31173 dynamic PET study were processed with a compartmental model that included two tissue compartments connected in parallel. Radioligand binding quantified by radioligand retention (80 min value to maximum value ratio) was compared to the binding parameters derived from the compartmental model. A binding ratio was calculated asDVR=DVS/DVNS, whereDVSandDVNSrepresented the distribution volumes of specific binding and nonspecific binding. Receptor binding was also determined by autoradiographyin vitro.Results. Correlations between rate constants and binding parameters derived by the convolution and deconvolution curve fittings were significant(r>0.9). Also significant was the correlation between the retention parameter derived from the tissue activity curve (Yret) and the retention parameter derived from the impulse response function (fret). Furthermore, significant correlations were found between these two retention parameters and DVR. Measurements with PET showed no significant changes in the radioligand binding parameters caused by IR, and thesein vivofindings were confirmed by autoradiography performedin vitro.Conclusions. Correlations between various binding parameters support the concept of the parallel connectivity compartmental model. If an arterial input function cannot be obtained, simple radioligand retention may be adequate for estimation ofin vivoradioligand binding.

scholarly journals 2-[3,5-Bis-(2-fluorobenzylidene)-4-piperidon-1-yl]-N-(4-fluorobenzyl)-acetamide and Its Evaluation as an Anticancer Agent

2013 ◽  
Vol 2013 ◽  
pp. 1-9
Author(s):  
Pallavi Lagisetty ◽  
Hrushikesh Agashe ◽  
Vibhudutta Awasthi
Keyword(s):  
Pet Imaging ◽  
Anticancer Agent ◽  
Tumor Regression ◽  
Radiochemical Yield ◽  
Emission Tomography ◽  
Whole Body ◽  
Positron Emission ◽  
Proliferation Assays ◽  
First Time ◽  
Kinetics Of

Synthesis of 2-[3,5-bis-(2-fluorobenzylidene)-4-piperidon-1-yl]-N-(4-fluorobenzyl)-acetamide, a derivative of 3,5-bis-(2-fluorobenzylidene)-4-piperidone (EF24), as an antiproliferative and imageable compound is described. The radioactive derivative was synthesized in 40–45% radiochemical yield using N-[4-fluoro(18F)benzyl]-2-bromoacetamide (NFLOBA) as a radiolabeled synthon for coupling with EF24. Cell proliferation assays showed that 2-[3,5-bis-(2-fluorobenzylidene)-4-piperidon-1-yl]-N-(4-fluorobenzyl)-acetamide (NFLOBA-EF24) had antiproliferative efficacy similar to that of EF24 in lung adenocarcinoma H441 cells.18F-NFLOBA-EF24 was investigated in normal rats for whole-body PET imaging and biodistribution. At necropsy after 1 h of injection, about 12% of injected compound was still circulating in blood; liver, kidney, and muscle were other tissues with moderate amounts of accumulation. In order to assess the tumor-suppressive activity, nonradioactive NFLOBA-EF24 was administered in nude rats carrying xenograft H441 tumor. After 15 days of treatment, the tumor size decreased by approximately 83% compared to the tumors in control rats. The tumor regression was also confirmed by molecular imaging of glucose metabolism with18F- fluorodeoxyglucose. The results suggest that EF24 could be efficiently modified with18F-labeled synthon NFLOBA for convenient PET imaging without altering the antitumor efficacy of the original compound. This study provides visual kinetics of synthetic curcuminoid EF24 by positron emission tomography for the first time.

Kinetics of Nanoparticle Radiolabeling of Metalloporphyrin with 64Cu for Positron Emission Tomography (PET) Imaging

2020 ◽  
Vol 59 (43) ◽  
pp. 19126-19132
Author(s):  
Leon Z. Wang ◽  
Tristan L. Lim ◽  
Prashanth K. Padakanti ◽  
Sean D. Carlin ◽  
Abass Alavi ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Pet Imaging ◽  
Emission Tomography ◽  
Positron Emission ◽  
Kinetics Of

scholarly journals TRACER KINETICS OF FLUORINE-18 LMI1195 COMPARED TO CARBON-11 HYDROXYEPHEDRINE FOR PET IMAGING OF SYMPATHETIC INNERVATION.

2018 ◽  
Vol 34 (10) ◽  
pp. S117-S118 ◽  
Author(s):  
J. Zelt ◽  
J. Renaud ◽  
L. Mielniczuk ◽  
L. Garrard ◽  
C. Orlandi ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Sympathetic Innervation ◽  
Tracer Kinetics ◽  
Kinetics Of

scholarly journals Impact of rifampicin-inhibitable transport on the liver distribution and tissue kinetics of erlotinib assessed with PET imaging in rats

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Dorra Amor ◽  
Sébastien Goutal ◽  
Solène Marie ◽  
Fabien Caillé ◽  
Martin Bauer ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Kinetics Of ◽  
Tissue Kinetics

scholarly journals Positron and γ-ray intensities in the decay of 45Ti

2015 ◽  
Vol 103 (6) ◽  
Author(s):  
Sebastian Kuhn ◽  
Ingo Spahn ◽  
Bernhard Scholten ◽  
Heinz H. Coenen
Keyword(s):  
Pet Imaging ◽  
Γ Ray ◽  
Positron Emitter ◽  
Imaging Properties

AbstractTo evaluate the PET-imaging properties of the promising positron emitter

Using immuno-PET imaging to monitor kinetics of T cell-mediated inflammation and treatment efficiency in a humanized mouse model for GvHD

2019 ◽  
Vol 47 (5) ◽  
pp. 1314-1325 ◽  
Author(s):  
Stefanie Pektor ◽  
Janine Schlöder ◽  
Benedikt Klasen ◽  
Nicole Bausbacher ◽  
Daniel-Christoph Wagner ◽  
...  
Keyword(s):  
T Cell ◽  
Mouse Model ◽  
Pet Imaging ◽  
Treatment Efficiency ◽  
Humanized Mouse ◽  
Humanized Mouse Model ◽  
Kinetics Of

scholarly journals Impact of P-Glycoprotein Function on the Brain Kinetics of the Weak Substrate 11C-Metoclopramide Assessed with PET Imaging in Humans

2019 ◽  
Vol 60 (7) ◽  
pp. 985-991 ◽  
Author(s):  
Nicolas Tournier ◽  
Martin Bauer ◽  
Verena Pichler ◽  
Lukas Nics ◽  
Eva-Maria Klebermass ◽  
...  
Keyword(s):  
Pet Imaging ◽  
P Glycoprotein ◽  
Kinetics Of ◽  
The Brain
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