Acoustic attenuation compensation in photoacoustic tomography: application to high-resolution 3D imaging of vascular networks in mice

2011 ◽  
Author(s):  
Bradley E. Treeby ◽  
Jan G. Laufer ◽  
Edward Z. Zhang ◽  
Francesca C. Norris ◽  
Mark F. Lythgoe ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Photoacoustic Tomography ◽  
Vascular Networks ◽  
Acoustic Attenuation ◽  
Attenuation Compensation

scholarly journals Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs

2020 ◽  
Author(s):  
Claire Walsh ◽  
Natalie Holroyd ◽  
Eoin Finnerty ◽  
Sean G. Ryan ◽  
Paul W. Sweeney ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Three Dimensional ◽  
Image Resolution ◽  
Biological Research ◽  
Wide Field ◽  
Vascular Networks ◽  
Optical Sectioning ◽  
Trade Offs ◽  
Xenograft Models

AbstractThree-dimensional microscopy of large biological samples (>0.5 cm3) is transforming biological research. Many existing techniques require trade-offs between image resolution and sample size, require clearing or use optical sectioning. These factors complicate the implementation of large volume 3D imaging. Here we present Multi-fluorescent High Resolution Episcopic Microscopy (MF-HREM) which allows 3D imaging of large samples without the need for clearing or optical sectioning.MF-HREM uses serial-sectioning and block-facing wide-field fluorescence, without the need for tissue clearing or optical sectioning. We detail developments in sample processing including stain penetration, resin embedding and imaging. In addition, we describe image post-processing methods needed to segment and further quantify these data. Finally, we demonstrate the wide applicability of MF-HREM by: 1) quantifying adult mouse glomeruli. 2) identifying injected cells and vascular networks in tumour xenograft models; 3) quantifying vascular networks and white matter track orientation in mouse brain.

High resolution 3D imaging of bump-bonds by means of synchrotron radiation computed laminography

2013 ◽  
Vol 8 (12) ◽  
pp. C12029-C12029 ◽  
Author(s):  
A Cecilia ◽  
E Hamann ◽  
T Koenig ◽  
F Xu ◽  
Y Cheng ◽  
...  
Keyword(s):  
High Resolution ◽  
Synchrotron Radiation ◽  
3D Imaging ◽  
Computed Laminography

High-resolution 3D imaging with a photonics-based broadband MIMO radar

2021 ◽  
Author(s):  
Bindong Gao ◽  
Fangzheng Zhang ◽  
Guanqun Sun ◽  
Shilong Pan
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Mimo Radar

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

2021 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Imaging ◽  
Imaging Technique ◽  
Ct Scanning ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Gastropod Species ◽  
Biological Studies ◽  
Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

Wide-angle, high-resolution three-dimensional (3D) imaging using non-mechanical beam steering

2021 ◽  
Author(s):  
Christopher L. Hoy ◽  
Jay Stockley ◽  
Kelly Kluttz ◽  
Doug McKnight ◽  
Lance Hosting ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Imaging ◽  
Beam Steering ◽  
Three Dimensional ◽  
Wide Angle

scholarly journals A Novel Orthogonal Waveform Separation Scheme for Airborne MIMO-SAR Systems

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3580 ◽  
Author(s):  
Jie Wang ◽  
Ke-Hong Zhu ◽  
Li-Na Wang ◽  
Xing-Dong Liang ◽  
Long-Yong Chen
Keyword(s):  
Remote Sensing ◽  
High Resolution ◽  
Synthetic Aperture Radar ◽  
3D Imaging ◽  
Synthetic Aperture ◽  
Separation Scheme ◽  
Sar Images ◽  
Wide Swath ◽  
Aperture Radar

In recent years, multi-input multi-output (MIMO) synthetic aperture radar (SAR) systems, which can promote the performance of 3D imaging, high-resolution wide-swath remote sensing, and multi-baseline interferometry, have received considerable attention. Several papers on MIMO-SAR have been published, but the research of such systems is seriously limited. This is mainly because the superposed echoes of the multiple transmitted orthogonal waveforms cannot be separated perfectly. The imperfect separation will introduce ambiguous energy and degrade SAR images dramatically. In this paper, a novel orthogonal waveform separation scheme based on echo-compression is proposed for airborne MIMO-SAR systems. Specifically, apart from the simultaneous transmissions, the transmitters are required to radiate several times alone in a synthetic aperture to sense their private inner-aperture channels. Since the channel responses at the neighboring azimuth positions are relevant, the energy of the solely radiated orthogonal waveforms in the superposed echoes will be concentrated. To this end, the echoes of the multiple transmitted orthogonal waveforms can be separated by cancelling the peaks. In addition, the cleaned echoes, along with original superposed one, can be used to reconstruct the unambiguous echoes. The proposed scheme is validated by simulations.

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