scholarly journals Subwavelength-resolution photoacoustic microscopy for label-free detection of optical absorption in vivo

2011 ◽  
Author(s):  
Chi Zhang ◽  
Konstantin Maslov ◽  
Lihong V. Wang
Keyword(s):  
Optical Absorption ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Label Free Detection ◽  
Subwavelength Resolution

scholarly journals Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

2010 ◽  
Vol 35 (19) ◽  
pp. 3195 ◽  
Author(s):  
Chi Zhang ◽  
Konstantin Maslov ◽  
Lihong V. Wang
Keyword(s):  
Optical Absorption ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Subwavelength Resolution

scholarly journals Recovery of photoacoustic images based on accurate ultrasound positioning

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yinhao Pan ◽  
Ningbo Chen ◽  
Liangjian Liu ◽  
Chengbo Liu ◽  
Zhiqiang Xu ◽  
...  
Keyword(s):  
Biological Tissue ◽  
Large Field ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Imaging Data ◽  
Motor Speed ◽  
Image Correction ◽  
Microscopy Imaging ◽  
Scanning Method

AbstractPhotoacoustic microscopy is an in vivo imaging technology based on the photoacoustic effect. It is widely used in various biomedical studies because it can provide high-resolution images while being label-free, safe, and harmless to biological tissue. Polygon-scanning is an effective scanning method in photoacoustic microscopy that can realize fast imaging of biological tissue with a large field of view. However, in polygon-scanning, fluctuations of the rotating motor speed and the geometric error of the rotating mirror cause image distortions, which seriously affect the photoacoustic-microscopy imaging quality. To improve the image quality of photoacoustic microscopy using polygon-scanning, an image correction method is proposed based on accurate ultrasound positioning. In this method, the photoacoustic and ultrasound imaging data of the sample are simultaneously obtained, and the angle information of each mirror used in the polygon-scanning is extracted from the ultrasonic data to correct the photoacoustic images. Experimental results show that the proposed method can significantly reduce image distortions in photoacoustic microscopy, with the image dislocation offset decreasing from 24.774 to 10.365 μm.

scholarly journals Dual-Polarized Fiber Laser Sensor for Photoacoustic Microscopy

Sensors ◽  
2019 ◽  
Vol 19 (21) ◽  
pp. 4632 ◽  
Author(s):  
Lin ◽  
Liang ◽  
Jin ◽  
Wang
Keyword(s):  
Fiber Laser ◽  
Optical Resolution ◽  
High Sensitivity ◽  
Low Noise ◽  
Laser Sensor ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Laser Sensors ◽  
Dual Polarized

Optical resolution photoacoustic microscopy (OR-PAM) provides high-resolution, label-free and non-invasive functional imaging for broad biomedical applications. Dual-polarized fiber laser sensors have high sensitivity, low noise, a miniature size, and excellent stability; thus, they have been used in acoustic detection in OR-PAM. Here, we review recent progress in fiber-laser-based ultrasound sensors for photoacoustic microscopy, especially the dual-polarized fiber laser sensor with high sensitivity. The principle, characterization and sensitivity optimization of this type of sensor are presented. In vivo experiments demonstrate its excellent performance in the detection of photoacoustic (PA) signals in OR-PAM. This review summarizes representative applications of fiber laser sensors in OR-PAM and discusses their further improvements.

scholarly journals Label-free photoacoustic microscopy for in-vivo tendon imaging using a fiber-based pulse laser

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hwi Don Lee ◽  
Jun Geun Shin ◽  
Hoon Hyun ◽  
Bong-Ahn Yu ◽  
Tae Joong Eom
Keyword(s):  
Pulse Laser ◽  
Photoacoustic Microscopy ◽  
Label Free

Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2015 ◽  
Author(s):  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
Hao He ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

Ultra-High Frequency Photoacoustic Microscopy: From Organelles to Organisms

2021 ◽  
Author(s):  
Michael J. Moore
Keyword(s):  
Optical Absorption ◽  
Imaging Techniques ◽  
Single Cells ◽  
Power Spectra ◽  
Photoacoustic Microscopy ◽  
Biological Cells ◽  
Zebrafish Larvae ◽  
Optical Attenuation ◽  
Absorption Potential

This dissertation describes novel signal analysis and imaging techniques for ultrahigh frequency (UHF, over 100 MHz) Photoacoustic Microscopy (PAM). New approaches for extracting information pertaining to object structure and scale are described, and novel sensing techniques and contrast mechanisms for imaging biological samples ranging from single cells to small organisms are presented. In the first section, I describe a methodology for assessing the structure of biological cells using UHF-PAM. The power spectra of ultrasound (US) pulses backscattered from MCF-7 cells, and photoacoustic (PA) waves emitted from their dyed nuclei were fit to analytical solutions to determine cell and nucleus diameter, respectively. The measured cell diameters (15.5±1.8 μm) and nucleus diameters (12±1.3 μm) were used to calculate the mean cell nucleus-to-cytoplasm ratio (1.9±1.0). Good agreement was observed between UHF-PAM measured values and literature. In the second section, I present a novel technique for PA image reconstruction that utilizes unique features in the PA power spectra as a source of contrast. The technique, termed F-Mode, provides a means for differentiating between objects of different scale that surpasses the capabilities of conventional reconstruction approaches. The ability of F-Mode to selectively accentuate absorbers of different size was demonstrated using experimental phantoms containing microspheres and cylindrical vessels, as well as in individual biological cells and live zebrafish larvae. Finally, I developed a new sensing technique, termed Photoacoustic Radiometry (PAR). Unlike PAM, which depicts optical absorption, PAR images depict the optical attenuation properties of the imaged object. It was demonstrated that PAR can be used to image transparent samples which generate no PA signals, and that simultaneous triplex PAR/PA/US imaging could be realized using our approach. Simultaneous PAR/PA imaging of biological cells, as well as zebrafish larvae in vivo, was also demonstrated. UHF-PAM provided excellent visualization of vascular organization in the larval trunk and head. The simultaneously acquired PAR images depicted anatomical structure (e.g. the notochord, muscle segments) not visible in PAM due to insufficient optical absorption. Potential areas of application for the new UHF-PAM techniques described in this dissertation include detection of cancer cells in blood samples, and investigation of tumour growth and metastasis. (6 zipped .mp4 files) https://digital.library.ryerson.ca/islandora/object/RULA:8618/datastream/Movies_Moore_mp4/view

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