Label free detection of pre-cancerous cells in vivo with a/LCI (Conference Presentation)

2019 ◽  
Author(s):  
Adam Wax
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
Cancerous Cells

Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2015 ◽  
Author(s):  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
Hao He ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals In vivo capture and label-free detection of early metastatic cells

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Samira M. Azarin ◽  
Ji Yi ◽  
Robert M. Gower ◽  
Brian A. Aguado ◽  
Megan E. Sullivan ◽  
...  
Keyword(s):  
Label Free ◽  
Metastatic Cells ◽  
Label Free Detection

scholarly journals Distinguishing dopamine and calcium responses using XNA-nanotube sensors for improved neurochemical sensing

2021 ◽  
Author(s):  
Alice J. Gillen ◽  
Alessandra Antonucci ◽  
Melania Reggente ◽  
Daniel Morales ◽  
Ardemis A. Boghossian
Keyword(s):  
Neurological Diseases ◽  
Locked Nucleic Acid ◽  
Optical Biosensors ◽  
Label Free ◽  
Sensing Applications ◽  
Label Free Detection ◽  
Improved Stability ◽  
New Applications

AbstractTo date, the engineering of single-stranded DNA-SWCNT (DNA-SWCNT) optical biosensors have largely focused on creating sensors for new applications with little focus on optimising existing sensors for in vitro and in vivo conditions. Recent studies have shown that nanotube fluorescence can be severely impacted by changes in local cation concentrations. This is particularly problematic for neurotransmitter sensing applications as spatial and temporal fluctuations in the concentration of cations, such as Na+, K+, or Ca2+, play a central role in neuromodulation. This can lead to inaccuracies in the determination of neurotransmitter concentrations using DNA-SWCNT sensors, which limits their use for detecting and treating neurological diseases.Herein, we present new approaches using locked nucleic acid (LNA) to engineer SWCNT sensors with improved stability towards cation-induced fluorescence changes. By incorporating LNA bases into the (GT)15-DNA sequence, we create sensors that are not only more resistant towards undesirable fluorescence modulation in the presence of Ca2+ but that also retain their capabilities for the label-free detection of dopamine. The synthetic biology approach presented in this work therefore serves as a complementary means for enhancing nanotube optoelectronic behavior, unlocking previously unexplored possibilities for developing nano-bioengineered sensors with augmented capabilities.

scholarly journals Towards in-vivo label-free detection of brain tumor margins with epi-illumination tomographic quantitative phase imaging

2021 ◽  
Vol 12 (3) ◽  
pp. 1621
Author(s):  
Paloma Casteleiro Costa ◽  
Zhe Guang ◽  
Patrick Ledwig ◽  
Zhaobin Zhang ◽  
Stewart Neill ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Phase Imaging ◽  
Label Free ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Tumor Margins ◽  
Label Free Detection

Label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

2016 ◽  
Author(s):  
Xiaoling Wang ◽  
Ping Yang ◽  
Rongrong Liu ◽  
Zhenyu Niu ◽  
Yuanzhen Suo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Melanoma Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals Recent advances in fluorescence-basedin vivoflow cytometry

2019 ◽  
Vol 12 (06) ◽  
pp. 1930008
Author(s):  
Kai Pang ◽  
Bobo Gu ◽  
Feng Liu ◽  
Mingli Dong ◽  
Lianqing Zhu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Detection Methods ◽  
Biological Applications ◽  
Label Free ◽  
Circulating Cells ◽  
Recent Advances ◽  
Label Free Detection ◽  
In Vivo Flow Cytometry ◽  
Biological Environment

The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.

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