Development of microfluidic system and optical tweezers for electrophysiological investigations of an individual cell

2010 ◽  
Author(s):  
A. Alrifaiy ◽  
N. Bitaraf ◽  
O. Lindahl ◽  
K. Ramser
Keyword(s):  
Optical Tweezers ◽  
Individual Cell ◽  
Microfluidic System

An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

Lab on a Chip ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 378-381 ◽  
Author(s):  
R. Burger ◽  
D. Kurzbuch ◽  
R. Gorkin ◽  
G. Kijanka ◽  
M. Glynn ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic System ◽  
Fluorescent Detection ◽  
Microfluidic Platform ◽  
Highly Efficient

In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers.

Complex Three-Dimensional Fluidic Reservoirs to Control Beads/Living Cells Contacts

2006 ◽  
Author(s):  
Serge Monneret ◽  
Federico Belloni ◽  
Olivier Soppera
Keyword(s):  
Optical Tweezers ◽  
Target Cell ◽  
Three Dimensional ◽  
Microfluidic System ◽  
Optical Traps ◽  
Multiple Point ◽  
Point Interactions ◽  
Cover Glass ◽  
Holographic Optical Tweezers ◽  
Microscope Cover Glass

In this paper, we combine holographic multiple optical tweezers with a three-dimensional microfluidic system to create a versatile microlaboratory. In order to determine cells local and/or temporal response to stimuli, and therefore draw their map of sensitivity, one convenient way is to apply antigen-covered latex beads in order to bind to plasma membrane, by means of optical tweezers. Using multiple optical traps could improve the efficiency of the measurements, but also their versatility. Therefore, we have developed a complete system based on holographic optical tweezers to realise multiple-point interactions between beads and cells with control of the stimulation places, timing, and durations. As we plan to use our system to study biological events in the hour timescale, we have to keep beads and cells separated, in order to prevent unwanted beads to circulate freely in the sample and bind to the target cell during the experiment. We then introduced microstereolithography as a 3D micro-manufacturing approach to the rapid prototyping of three-dimensional fluidic microchambers of complex shapes inside the sample, comprising wells, channels and walls to inject beads locally and keep them separated from cells in our assays. We demonstrated the possibility for microSL to easily and rapidly (typically one hour) fabricate small and three-dimensional observation chambers with customized design of the flow channels, including fluidic reservoirs of typically 500–1500 μm diameter, 5–12 mm height, in order to facilitate manual filling. Several shapes of reservoirs designed to keep beads and cells separated in liquid samples have been realized and successfully tested. Some of them included up to 3 reservoirs, in order to allow co-distribution of different types of beads. Each reservoir typically contained 2–10 μl of solution holding the beads, with a horizontal outlet of 100–200 μm in diameter which allows beads to deposit locally on the microscope cover glass placed under the reservoir outlet. Limited extension of beads under the outlet on the glass has been confirmed, and the ability of the polymeric structures to confine beads in a restricted area has been demonstrated. In the following we present examples of manipulations by multiple holographic optical tweezers consisting at first in extracting several beads from such an area by going through an aperture designed in the structure, making them travel to the target cell, and finally depositing on its outer membrane.

scholarly journals Optical tweezers applied to a microfluidic system

Lab on a Chip ◽  
2004 ◽  
Vol 4 (3) ◽  
pp. 196-200 ◽  
Author(s):  
Jonas Enger ◽  
Mattias Goksör ◽  
Kerstin Ramser ◽  
Petter Hagberg ◽  
Dag Hanstorp
Keyword(s):  
Optical Tweezers ◽  
Microfluidic System

Microfluidic system for single cell sorting with optical tweezers

2010 ◽  
Author(s):  
Thomas Bruns ◽  
Laszlo Becsi ◽  
Marc Talkenberg ◽  
Michael Wagner ◽  
Petra Weber ◽  
...  
Keyword(s):  
Single Cell ◽  
Optical Tweezers ◽  
Cell Sorting ◽  
Microfluidic System

A microfluidic system for studies of stress response in single cells using optical tweezers

2006 ◽  
Author(s):  
Annette Granéli ◽  
Emma Eriksson ◽  
Jonas Enger ◽  
Kerstin Ramser ◽  
Mattias Goksör ◽  
...  
Keyword(s):  
Stress Response ◽  
Optical Tweezers ◽  
Single Cells ◽  
Microfluidic System

A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes

Lab on a Chip ◽  
2007 ◽  
Vol 7 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Emma Eriksson ◽  
Jonas Enger ◽  
Bodil Nordlander ◽  
Nika Erjavec ◽  
Kerstin Ramser ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Environmental Changes ◽  
Single Cells ◽  
Microfluidic System ◽  
Cytological Alterations

Ultrastructure of myoepithelial cell in parotid gland

1988 ◽  
Vol 46 ◽  
pp. 342-343
Author(s):  
C. N. Sun
Keyword(s):  
Parotid Gland ◽  
Osmium Tetroxide ◽  
Individual Cell ◽  
Branching Processes ◽  
Muscle Cells ◽  
Myoepithelial Cells ◽  
Uranyl Acetate ◽  
Thin Sections ◽  
Gland Carcinoma ◽  
Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

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