A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes

Lab on a Chip ◽  
2007 ◽  
Vol 7 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Emma Eriksson ◽  
Jonas Enger ◽  
Bodil Nordlander ◽  
Nika Erjavec ◽  
Kerstin Ramser ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Environmental Changes ◽  
Single Cells ◽  
Microfluidic System ◽  
Cytological Alterations

A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning

Lab on a Chip ◽  
2010 ◽  
Vol 10 (5) ◽  
pp. 617-625 ◽  
Author(s):  
Emma Eriksson ◽  
Kristin Sott ◽  
Fredrik Lundqvist ◽  
Martin Sveningsson ◽  
Jan Scrimgeour ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Environmental Changes ◽  
Single Cells ◽  
Cell Selection

A microfluidic system for studies of stress response in single cells using optical tweezers

2006 ◽  
Author(s):  
Annette Granéli ◽  
Emma Eriksson ◽  
Jonas Enger ◽  
Kerstin Ramser ◽  
Mattias Goksör ◽  
...  
Keyword(s):  
Stress Response ◽  
Optical Tweezers ◽  
Single Cells ◽  
Microfluidic System

scholarly journals Measurement of dynamic membrane mechanosensation using optical tweezers

2021 ◽  
Author(s):  
Xuanling Li ◽  
Xing Liu ◽  
Xiaoyu Song ◽  
Yinmei Li ◽  
Ming Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Environmental Changes ◽  
Quantitative Measure ◽  
Relaxation Curve ◽  
Membrane Dynamics ◽  
Molecular Organization ◽  
Cytoskeletal Remodeling ◽  
Relaxation Curves ◽  
Membrane Tether

Abstract Many cellular processes are orchestrated by dynamic changes in the plasma membrane to form membrane projections and endocytic vesicles in response to extracellular environmental changes. Our previous studies show that ARF6-ACAP4-ezrin signaling regulates membrane dynamics and curvature in response to EGF stimulation. However, there is no quantitative measurement to relate molecular organization of membrane cytoskeletal remodeling to stimulus-elicited mechanosensation on the plasma membrane. Optical tweezers is a powerful tool in the study of membrane tension. Comparing to pulling out an entire membrane tether at one time, the step-like method is more efficient because multiple relaxation curves can be obtained from one membrane tether. Fewer models describe relaxation curves to characterize mechanical properties of cell membrane. Here we establish a new method to measure the membrane relaxation curve of HeLa cells judged by the relationship between membrane tether diameter and tensions. We obtained effective viscosities and static tensions by fitting relaxation curves to our model. We noticed the delicate structure of relaxation curves contains information of cytoskeletal remodeling and lateral protein diffusion. Our study established a quantitative measure to characterize the mechanosensation of epithelial cells in response to stimulus-elicited membrane dynamics.

scholarly journals Metabolic cost of rapid adaptation of single yeast cells

2020 ◽  
Vol 117 (20) ◽  
pp. 10660-10666 ◽  
Author(s):  
Gabrielle Woronoff ◽  
Philippe Nghe ◽  
Jean Baudry ◽  
Laurent Boitard ◽  
Erez Braun ◽  
...  
Keyword(s):  
Cell Metabolism ◽  
Single Cells ◽  
A Priori ◽  
Metabolic Cost ◽  
Microfluidic System ◽  
Cellular Reprogramming ◽  
Yeast Cells ◽  
Rapid Adaptation ◽  
Metabolic Coupling ◽  
Induced Changes

Cells can rapidly adapt to changing environments through nongenetic processes; however, the metabolic cost of such adaptation has never been considered. Here we demonstrate metabolic coupling in a remarkable, rapid adaptation process (1 in 1,000 cells adapt per hour) by simultaneously measuring metabolism and division of thousands of individual Saccharomyces cerevisiae cells using a droplet microfluidic system: droplets containing single cells are immobilized in a two-dimensional (2D) array, with osmotically induced changes in droplet volume being used to measure cell metabolism, while simultaneously imaging the cells to measure division. Following a severe challenge, most cells, while not dividing, continue to metabolize, displaying a remarkably wide diversity of metabolic trajectories from which adaptation events can be anticipated. Adaptation requires a characteristic amount of energy, indicating that it is an active process. The demonstration that metabolic trajectories predict a priori adaptation events provides evidence of tight energetic coupling between metabolism and regulatory reorganization in adaptation. This process allows S. cerevisiae to adapt on a physiological timescale, but related phenomena may also be important in other processes, such as cellular differentiation, cellular reprogramming, and the emergence of drug resistance in cancer.

scholarly journals Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers

2010 ◽  
Vol 3 (3) ◽  
pp. 213-228 ◽  
Author(s):  
Nathalie Nève ◽  
Sean S. Kohles ◽  
Shelley R. Winn ◽  
Derek C. Tretheway
Keyword(s):  
Optical Tweezers ◽  
Single Cells

scholarly journals An acoustofluidic trap and transfer approach for organizing a high density single cell array

Lab on a Chip ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 2124-2133 ◽  
Author(s):  
Korine A. Ohiri ◽  
Sean T. Kelly ◽  
Jeffrey D. Motschman ◽  
Kevin H. Lin ◽  
Kris C. Wood ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Microfluidic System ◽  
High Density ◽  
Cell Array

We demonstrate a hybrid microfluidic system that combines fluidic trapping and acoustic switching to organize an array of single cells at high density.

scholarly journals Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1759 ◽  
Author(s):  
Takehiro Yamazaki ◽  
Toshifumi Kishimoto ◽  
Paweł Leszczyński ◽  
Koichiro Sadakane ◽  
Takahiro Kenmotsu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Optical Tweezers ◽  
Single Cells ◽  
Three Dimensional ◽  
Cell Types ◽  
Cellular Interactions ◽  
Research Fields ◽  
Wide Range

To better understand the regulation and function of cellular interactions, three-dimensional (3D) assemblies of single cells and subsequent functional analysis are gaining popularity in many research fields. While we have developed strategies to build stable cellular structures using optical tweezers in a minimally invasive state, methods for manipulating a wide range of cell types have yet to be established. To mimic organ-like structures, the construction of 3D cellular assemblies with variety of cell types is essential. Our recent studies have shown that the presence of nonspecific soluble polymers in aqueous solution is the key to creating stable 3D cellular assemblies efficiently. The present study further expands on the construction of 3D single cell assemblies using two different cell types. We have successfully generated 3D cellular assemblies, using GFP-labeled adipose tissue-derived stem cells and endothelial cells by using optical tweezers. Our findings will support the development of future applications to further characterize cellular interactions in tissue regeneration.

scholarly journals Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 308 ◽  
Author(s):  
Phalguni Tewari Kumar ◽  
Deborah Decrop ◽  
Saba Safdar ◽  
Ioannis Passaris ◽  
Tadej Kokalj ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Cell Sorting ◽  
Magnetic Beads ◽  
Single Cells ◽  
Digital Microfluidics ◽  
Cell Behavior ◽  
Bacterial Cells ◽  
Microfluidic Platforms ◽  
Dynamic Cell ◽  
Downstream Analysis

When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled methods have typically been used. However, the former, although allowing cell sorting, fails to track dynamic cell behavior, while the latter has been limited to complex channel-based microfluidic platforms. In this study, digital microfluidics (DMF) was integrated with OT for selective trapping, relocation, and further proliferation of single bacterial cells, while offering continuous imaging of cells to evaluate dynamic cell behavior. To enable this, magnetic beads coated with Salmonella Typhimurium-targeting antibodies were seeded in the microwell array of the DMF platform, and used to capture single cells of a fluorescent S. Typhimurium population. Next, OT were used to select a bead with a bacterium of interest, based on its fluorescent expression, and to relocate this bead to a different microwell on the same or different array. Using an agar patch affixed on top, the relocated bacterium was subsequently allowed to proliferate. Our OT-integrated DMF platform thus successfully enabled selective trapping, retrieval, relocation, and proliferation of bacteria of interest at single-cell level, thereby enabling their downstream analysis.

scholarly journals Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System

Sensors ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 2763-2773 ◽  
Author(s):  
Yang Zhao ◽  
Deyong Chen ◽  
Yana Luo ◽  
Feng Chen ◽  
Xiaoting Zhao ◽  
...  
Keyword(s):  
Young’S Modulus ◽  
Young's Modulus ◽  
Single Cells ◽  
Microfluidic System ◽  
Membrane Capacitance ◽  
Specific Membrane
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